Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2957 and PP_2958 are separated by 91 nucleotides PP_2958 and PP_2959 are separated by 13 nucleotides PP_2959 and PP_2960 are separated by 129 nucleotides
PP_2957: PP_2957 - Acetyltransferase, GNAT family, at 3,361,361 to 3,362,455
_2957
PP_2958: PP_2958 - Glutaredoxin, at 3,362,547 to 3,362,801
_2958
PP_2959: PP_2959 - Alkylhydroperoxidase AhpD domain protein, at 3,362,815 to 3,363,333
_2959
PP_2960: PP_2960 - transcriptional regulator, AraC family, at 3,363,463 to 3,364,284
_2960
Position (kb)
3362
3363
3364 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3361.841 kb on + strand, within PP_2957 at 3361.841 kb on + strand, within PP_2957 at 3361.844 kb on + strand, within PP_2957 at 3361.845 kb on - strand, within PP_2957 at 3362.113 kb on - strand, within PP_2957 at 3362.237 kb on - strand, within PP_2957 at 3362.309 kb on + strand, within PP_2957 at 3362.310 kb on - strand, within PP_2957 at 3362.377 kb on - strand at 3362.474 kb on + strand at 3362.475 kb on - strand at 3362.475 kb on - strand at 3362.478 kb on + strand at 3362.479 kb on - strand at 3362.479 kb on - strand at 3362.506 kb on + strand at 3362.506 kb on + strand at 3362.506 kb on + strand at 3362.507 kb on - strand at 3362.507 kb on - strand at 3362.507 kb on - strand at 3362.634 kb on + strand, within PP_2958 at 3362.634 kb on + strand, within PP_2958 at 3362.634 kb on + strand, within PP_2958 at 3362.635 kb on - strand, within PP_2958 at 3362.761 kb on + strand, within PP_2958 at 3362.780 kb on + strand at 3362.780 kb on + strand at 3362.781 kb on - strand at 3362.781 kb on - strand at 3362.781 kb on - strand at 3362.782 kb on + strand at 3362.782 kb on + strand at 3362.782 kb on + strand at 3362.782 kb on + strand at 3362.782 kb on + strand at 3362.782 kb on + strand at 3362.783 kb on - strand at 3362.783 kb on - strand at 3362.783 kb on - strand at 3362.783 kb on - strand at 3362.783 kb on - strand at 3362.783 kb on - strand at 3362.786 kb on + strand at 3362.786 kb on + strand at 3362.786 kb on + strand at 3362.787 kb on - strand at 3362.787 kb on - strand at 3362.808 kb on + strand at 3362.817 kb on - strand at 3362.867 kb on + strand, within PP_2959 at 3362.868 kb on - strand, within PP_2959 at 3362.920 kb on - strand, within PP_2959 at 3362.925 kb on + strand, within PP_2959 at 3362.926 kb on - strand, within PP_2959 at 3363.168 kb on + strand, within PP_2959 at 3363.168 kb on + strand, within PP_2959 at 3363.169 kb on - strand, within PP_2959 at 3363.322 kb on - strand at 3363.324 kb on + strand at 3363.325 kb on - strand at 3363.348 kb on - strand at 3363.369 kb on + strand at 3363.387 kb on + strand at 3363.387 kb on + strand at 3363.387 kb on + strand at 3363.387 kb on + strand at 3363.387 kb on + strand at 3363.387 kb on + strand at 3363.388 kb on - strand at 3363.388 kb on - strand at 3363.388 kb on - strand at 3363.390 kb on + strand at 3363.390 kb on + strand at 3363.391 kb on - strand at 3363.391 kb on - strand at 3363.431 kb on + strand at 3363.431 kb on + strand at 3363.432 kb on - strand at 3363.432 kb on - strand at 3363.432 kb on - strand at 3363.482 kb on + strand at 3363.482 kb on + strand at 3363.482 kb on + strand at 3363.483 kb on - strand at 3363.486 kb on + strand at 3363.487 kb on - strand at 3363.487 kb on - strand at 3363.507 kb on + strand at 3363.710 kb on + strand, within PP_2960 at 3363.711 kb on - strand, within PP_2960 at 3364.101 kb on + strand, within PP_2960 at 3364.101 kb on + strand, within PP_2960 at 3364.101 kb on + strand, within PP_2960 at 3364.102 kb on - strand, within PP_2960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,361,841 + PP_2957 0.44 +1.1 3,361,841 + PP_2957 0.44 -3.2 3,361,844 + PP_2957 0.44 +1.6 3,361,845 - PP_2957 0.44 -0.3 3,362,113 - PP_2957 0.69 -0.7 3,362,237 - PP_2957 0.80 -0.1 3,362,309 + PP_2957 0.87 +1.0 3,362,310 - PP_2957 0.87 +1.0 3,362,377 - +0.4 3,362,474 + -0.2 3,362,475 - +0.8 3,362,475 - -1.0 3,362,478 + +0.2 3,362,479 - +1.2 3,362,479 - +0.5 3,362,506 + -1.3 3,362,506 + +2.0 3,362,506 + -0.5 3,362,507 - -3.7 3,362,507 - +0.7 3,362,507 - -1.4 3,362,634 + PP_2958 0.34 -2.3 3,362,634 + PP_2958 0.34 +0.6 3,362,634 + PP_2958 0.34 +0.6 3,362,635 - PP_2958 0.35 -1.0 3,362,761 + PP_2958 0.84 +0.2 3,362,780 + -1.5 3,362,780 + +1.4 3,362,781 - +1.4 3,362,781 - +0.0 3,362,781 - -0.1 3,362,782 + +0.9 3,362,782 + +0.7 3,362,782 + +0.3 3,362,782 + -0.6 3,362,782 + -0.5 3,362,782 + -1.0 3,362,783 - -0.3 3,362,783 - +1.1 3,362,783 - -3.7 3,362,783 - -0.5 3,362,783 - -0.6 3,362,783 - -0.2 3,362,786 + -0.9 3,362,786 + -0.8 3,362,786 + +0.3 3,362,787 - -1.4 3,362,787 - -0.5 3,362,808 + +1.2 3,362,817 - -0.9 3,362,867 + PP_2959 0.10 +1.2 3,362,868 - PP_2959 0.10 -0.2 3,362,920 - PP_2959 0.20 +0.6 3,362,925 + PP_2959 0.21 -0.1 3,362,926 - PP_2959 0.21 -0.5 3,363,168 + PP_2959 0.68 +0.7 3,363,168 + PP_2959 0.68 +2.3 3,363,169 - PP_2959 0.68 +0.7 3,363,322 - +1.1 3,363,324 + +0.2 3,363,325 - +2.5 3,363,348 - -0.1 3,363,369 + -1.6 3,363,387 + +0.3 3,363,387 + -0.1 3,363,387 + +1.6 3,363,387 + +0.5 3,363,387 + +0.1 3,363,387 + +1.0 3,363,388 - +1.4 3,363,388 - +0.6 3,363,388 - -2.4 3,363,390 + +0.7 3,363,390 + +0.9 3,363,391 - -0.3 3,363,391 - -1.2 3,363,431 + +0.7 3,363,431 + -0.2 3,363,432 - -4.3 3,363,432 - +1.8 3,363,432 - -1.3 3,363,482 + -0.3 3,363,482 + -1.2 3,363,482 + +2.3 3,363,483 - +0.7 3,363,486 + +0.1 3,363,487 - -2.0 3,363,487 - +0.9 3,363,507 + +3.1 3,363,710 + PP_2960 0.30 -2.2 3,363,711 - PP_2960 0.30 -0.1 3,364,101 + PP_2960 0.78 +0.3 3,364,101 + PP_2960 0.78 -0.9 3,364,101 + PP_2960 0.78 -1.3 3,364,102 - PP_2960 0.78 +0.9
Or see this region's nucleotide sequence