Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2715 and PP_2716 are separated by 13 nucleotides PP_2716 and PP_2717 are separated by 28 nucleotides PP_2717 and PP_2718 are separated by 21 nucleotides PP_2718 and PP_2719 are separated by 185 nucleotides PP_2719 and PP_2720 are separated by 73 nucleotides PP_2720 and PP_2721 overlap by 22 nucleotides
PP_2715: PP_2715 - ArsH protein, at 3,104,850 to 3,105,551
_2715
PP_2716: PP_2716 - arsenate reductase, at 3,105,565 to 3,106,035
_2716
PP_2717: PP_2717 - arsenite/antimonite transporter, at 3,106,064 to 3,107,347
_2717
PP_2718: PP_2718 - arsenic resistance transcriptional regulator, at 3,107,369 to 3,107,716
_2718
PP_2719: PP_2719 - conserved protein of unknown function, at 3,107,902 to 3,108,006
_2719
PP_2720: PP_2720 - conserved protein of unknown function, at 3,108,080 to 3,108,301
_2720
PP_2721: PP_2721 - conserved membrane protein of unknown function, at 3,108,280 to 3,108,630
_2721
Position (kb)
3106
3107
3108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3105.325 kb on - strand, within PP_2715 at 3105.414 kb on + strand, within PP_2715 at 3105.414 kb on + strand, within PP_2715 at 3105.454 kb on + strand, within PP_2715 at 3105.697 kb on - strand, within PP_2716 at 3105.697 kb on - strand, within PP_2716 at 3105.873 kb on + strand, within PP_2716 at 3105.874 kb on - strand, within PP_2716 at 3106.143 kb on + strand at 3106.143 kb on + strand at 3106.143 kb on + strand at 3106.144 kb on - strand at 3106.144 kb on - strand at 3106.147 kb on - strand at 3106.261 kb on - strand, within PP_2717 at 3106.277 kb on + strand, within PP_2717 at 3106.467 kb on + strand, within PP_2717 at 3106.479 kb on + strand, within PP_2717 at 3106.480 kb on - strand, within PP_2717 at 3106.480 kb on - strand, within PP_2717 at 3106.480 kb on - strand, within PP_2717 at 3106.731 kb on + strand at 3106.766 kb on + strand, within PP_2717 at 3107.034 kb on - strand, within PP_2717 at 3107.051 kb on + strand, within PP_2717 at 3107.202 kb on - strand, within PP_2717 at 3107.229 kb on - strand at 3107.229 kb on - strand at 3107.309 kb on + strand at 3107.309 kb on + strand at 3107.514 kb on + strand, within PP_2718 at 3107.515 kb on - strand, within PP_2718 at 3107.515 kb on - strand, within PP_2718 at 3107.768 kb on - strand at 3107.875 kb on + strand at 3107.875 kb on + strand at 3107.875 kb on + strand at 3107.876 kb on - strand at 3107.877 kb on + strand at 3107.878 kb on - strand at 3107.878 kb on - strand at 3107.941 kb on + strand, within PP_2719 at 3108.253 kb on - strand, within PP_2720 at 3108.337 kb on - strand, within PP_2721
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,105,325 - PP_2715 0.68 -1.8 3,105,414 + PP_2715 0.80 +0.3 3,105,414 + PP_2715 0.80 -0.4 3,105,454 + PP_2715 0.86 -0.6 3,105,697 - PP_2716 0.28 +1.2 3,105,697 - PP_2716 0.28 +0.1 3,105,873 + PP_2716 0.65 +0.2 3,105,874 - PP_2716 0.66 -2.2 3,106,143 + -0.8 3,106,143 + +0.9 3,106,143 + +0.1 3,106,144 - +1.0 3,106,144 - -1.9 3,106,147 - -1.7 3,106,261 - PP_2717 0.15 +0.0 3,106,277 + PP_2717 0.17 -1.8 3,106,467 + PP_2717 0.31 +0.9 3,106,479 + PP_2717 0.32 +1.2 3,106,480 - PP_2717 0.32 -2.5 3,106,480 - PP_2717 0.32 +0.2 3,106,480 - PP_2717 0.32 +1.6 3,106,731 + +2.1 3,106,766 + PP_2717 0.55 +1.1 3,107,034 - PP_2717 0.76 -2.7 3,107,051 + PP_2717 0.77 +1.3 3,107,202 - PP_2717 0.89 +1.5 3,107,229 - -0.0 3,107,229 - -0.8 3,107,309 + +1.4 3,107,309 + +0.7 3,107,514 + PP_2718 0.42 -0.0 3,107,515 - PP_2718 0.42 +0.1 3,107,515 - PP_2718 0.42 -2.8 3,107,768 - +0.2 3,107,875 + -0.7 3,107,875 + +1.4 3,107,875 + +0.4 3,107,876 - -0.3 3,107,877 + +0.8 3,107,878 - +0.5 3,107,878 - -1.7 3,107,941 + PP_2719 0.37 +1.5 3,108,253 - PP_2720 0.78 -1.0 3,108,337 - PP_2721 0.16 -0.5
Or see this region's nucleotide sequence