Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2460 and PP_2461 overlap by 29 nucleotides PP_2461 and PP_2462 are separated by 220 nucleotides PP_2462 and PP_2463 are separated by 200 nucleotides PP_2463 and PP_5525 are separated by 162 nucleotides
PP_2460: PP_2460 - ribonucleoside hydrolase, at 2,806,627 to 2,807,595
_2460
PP_2461: PP_2461 - conserved protein of unknown function, at 2,807,567 to 2,807,779
_2461
PP_2462: PP_2462 - conserved protein of unknown function, at 2,808,000 to 2,808,374
_2462
PP_2463: PP_2463 - nucleic acid cold-shock chaperone, at 2,808,575 to 2,808,787
_2463
PP_5525: PP_5525 - conserved exported protein of unknown function, at 2,808,950 to 2,809,255
_5525
Position (kb)
2807
2808
2809 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2807.164 kb on + strand, within PP_2460 at 2807.218 kb on + strand, within PP_2460 at 2807.329 kb on + strand, within PP_2460 at 2807.329 kb on + strand, within PP_2460 at 2807.329 kb on + strand, within PP_2460 at 2807.329 kb on + strand, within PP_2460 at 2807.330 kb on - strand, within PP_2460 at 2807.330 kb on - strand, within PP_2460 at 2807.353 kb on + strand, within PP_2460 at 2807.353 kb on + strand, within PP_2460 at 2807.354 kb on - strand, within PP_2460 at 2807.354 kb on - strand, within PP_2460 at 2807.360 kb on + strand, within PP_2460 at 2807.360 kb on + strand, within PP_2460 at 2807.361 kb on - strand, within PP_2460 at 2807.399 kb on - strand, within PP_2460 at 2807.405 kb on + strand, within PP_2460 at 2807.405 kb on + strand, within PP_2460 at 2807.610 kb on + strand, within PP_2461 at 2807.901 kb on + strand at 2807.908 kb on - strand at 2807.921 kb on + strand at 2807.921 kb on + strand at 2807.922 kb on - strand at 2807.922 kb on - strand at 2807.922 kb on - strand at 2807.922 kb on - strand at 2808.073 kb on - strand, within PP_2462 at 2808.073 kb on - strand, within PP_2462 at 2808.431 kb on + strand at 2808.431 kb on + strand at 2808.431 kb on + strand at 2808.431 kb on + strand at 2808.432 kb on - strand at 2808.480 kb on + strand at 2808.567 kb on + strand at 2808.567 kb on + strand at 2808.568 kb on - strand at 2808.693 kb on - strand, within PP_2463 at 2808.808 kb on + strand at 2808.809 kb on - strand at 2808.866 kb on + strand at 2808.880 kb on + strand at 2808.880 kb on + strand at 2808.890 kb on + strand at 2808.891 kb on - strand at 2808.944 kb on - strand at 2809.155 kb on + strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.263 kb on + strand at 2809.264 kb on - strand at 2809.292 kb on + strand at 2809.292 kb on + strand at 2809.293 kb on - strand at 2809.332 kb on - strand at 2809.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,807,164 + PP_2460 0.55 -0.8 2,807,218 + PP_2460 0.61 +1.2 2,807,329 + PP_2460 0.72 -1.6 2,807,329 + PP_2460 0.72 -0.3 2,807,329 + PP_2460 0.72 +0.2 2,807,329 + PP_2460 0.72 +1.2 2,807,330 - PP_2460 0.73 +0.3 2,807,330 - PP_2460 0.73 +0.7 2,807,353 + PP_2460 0.75 -1.8 2,807,353 + PP_2460 0.75 +0.7 2,807,354 - PP_2460 0.75 +0.1 2,807,354 - PP_2460 0.75 -0.4 2,807,360 + PP_2460 0.76 -1.3 2,807,360 + PP_2460 0.76 -0.5 2,807,361 - PP_2460 0.76 -1.4 2,807,399 - PP_2460 0.80 -3.3 2,807,405 + PP_2460 0.80 +0.9 2,807,405 + PP_2460 0.80 -0.7 2,807,610 + PP_2461 0.20 -0.0 2,807,901 + -2.7 2,807,908 - +0.2 2,807,921 + -1.0 2,807,921 + -3.4 2,807,922 - -0.6 2,807,922 - +1.3 2,807,922 - -1.2 2,807,922 - -0.5 2,808,073 - PP_2462 0.19 +0.2 2,808,073 - PP_2462 0.19 +1.3 2,808,431 + -3.9 2,808,431 + -0.2 2,808,431 + -1.1 2,808,431 + +1.0 2,808,432 - +0.6 2,808,480 + +0.4 2,808,567 + +1.3 2,808,567 + -0.4 2,808,568 - -1.0 2,808,693 - PP_2463 0.55 -1.6 2,808,808 + -0.6 2,808,809 - +0.5 2,808,866 + +1.0 2,808,880 + +2.5 2,808,880 + +0.7 2,808,890 + -1.8 2,808,891 - +0.7 2,808,944 - +0.7 2,809,155 + PP_5525 0.67 +0.3 2,809,156 - PP_5525 0.67 -1.2 2,809,156 - PP_5525 0.67 -0.9 2,809,156 - PP_5525 0.67 -2.1 2,809,156 - PP_5525 0.67 -0.6 2,809,156 - PP_5525 0.67 -0.0 2,809,263 + +0.5 2,809,264 - -1.0 2,809,292 + -2.9 2,809,292 + +1.1 2,809,293 - -0.0 2,809,332 - +0.0 2,809,332 - +0.6
Or see this region's nucleotide sequence