Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2377 and PP_2378 are separated by 58 nucleotides PP_2378 and PP_2379 are separated by 196 nucleotides PP_2379 and PP_2380 are separated by 0 nucleotides PP_2380 and PP_2381 are separated by 49 nucleotides
PP_2377: PP_2377 - putative acetyltransferase Act, at 2,717,781 to 2,718,815
_2377
PP_2378: PP_2378 - Fe/S biogenesis protein NfuA, at 2,718,874 to 2,719,458
_2378
PP_2379: PP_2379 - putative cytochrome oxidase biogenesis protein, at 2,719,655 to 2,720,263
_2379
PP_2380: PP_2380 - conserved exported protein of unknown function, at 2,720,264 to 2,720,746
_2380
PP_2381: PP_2381 - conserved protein of unknown function, at 2,720,796 to 2,721,038
_2381
Position (kb)
2719
2720
2721 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2718.697 kb on + strand, within PP_2377 at 2718.697 kb on + strand, within PP_2377 at 2718.698 kb on - strand, within PP_2377 at 2718.698 kb on - strand, within PP_2377 at 2718.714 kb on + strand at 2718.739 kb on - strand at 2718.842 kb on + strand at 2718.843 kb on - strand at 2718.872 kb on - strand at 2718.882 kb on + strand at 2718.882 kb on + strand at 2718.882 kb on + strand at 2718.883 kb on - strand at 2718.883 kb on - strand at 2718.884 kb on + strand at 2718.884 kb on + strand at 2718.885 kb on - strand at 2718.885 kb on - strand at 2719.010 kb on - strand, within PP_2378 at 2719.092 kb on - strand at 2719.121 kb on - strand, within PP_2378 at 2719.212 kb on + strand, within PP_2378 at 2719.212 kb on + strand, within PP_2378 at 2719.213 kb on - strand, within PP_2378 at 2719.219 kb on + strand at 2719.219 kb on + strand at 2719.219 kb on + strand at 2719.220 kb on - strand, within PP_2378 at 2719.220 kb on - strand at 2719.220 kb on - strand, within PP_2378 at 2719.220 kb on - strand, within PP_2378 at 2719.226 kb on + strand, within PP_2378 at 2719.227 kb on - strand at 2719.393 kb on - strand, within PP_2378 at 2719.450 kb on + strand at 2719.450 kb on + strand at 2719.453 kb on + strand at 2719.641 kb on + strand at 2719.641 kb on + strand at 2719.757 kb on + strand, within PP_2379 at 2719.845 kb on - strand, within PP_2379 at 2720.022 kb on - strand, within PP_2379 at 2720.109 kb on - strand, within PP_2379 at 2720.135 kb on + strand, within PP_2379 at 2720.159 kb on - strand, within PP_2379 at 2720.159 kb on - strand, within PP_2379 at 2720.162 kb on + strand, within PP_2379 at 2720.162 kb on + strand, within PP_2379 at 2720.162 kb on + strand, within PP_2379 at 2720.163 kb on - strand, within PP_2379 at 2720.163 kb on - strand, within PP_2379 at 2720.163 kb on - strand, within PP_2379 at 2720.163 kb on - strand, within PP_2379 at 2720.163 kb on - strand, within PP_2379 at 2720.169 kb on - strand, within PP_2379 at 2720.169 kb on - strand, within PP_2379 at 2720.304 kb on + strand at 2720.305 kb on - strand at 2720.305 kb on - strand at 2721.055 kb on - strand at 2721.145 kb on - strand at 2721.149 kb on - strand at 2721.149 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,718,697 + PP_2377 0.89 +0.4 2,718,697 + PP_2377 0.89 -0.6 2,718,698 - PP_2377 0.89 +1.0 2,718,698 - PP_2377 0.89 +0.6 2,718,714 + -1.6 2,718,739 - -1.8 2,718,842 + -0.4 2,718,843 - +0.7 2,718,872 - -0.4 2,718,882 + -1.5 2,718,882 + -0.7 2,718,882 + -1.1 2,718,883 - -1.0 2,718,883 - -1.6 2,718,884 + -2.5 2,718,884 + -1.3 2,718,885 - -1.5 2,718,885 - -0.5 2,719,010 - PP_2378 0.23 -0.8 2,719,092 - +0.3 2,719,121 - PP_2378 0.42 -1.0 2,719,212 + PP_2378 0.58 -1.5 2,719,212 + PP_2378 0.58 -1.2 2,719,213 - PP_2378 0.58 -1.1 2,719,219 + +2.2 2,719,219 + -1.1 2,719,219 + +1.1 2,719,220 - PP_2378 0.59 -0.9 2,719,220 - -1.7 2,719,220 - PP_2378 0.59 -1.3 2,719,220 - PP_2378 0.59 -0.3 2,719,226 + PP_2378 0.60 +0.9 2,719,227 - +3.1 2,719,393 - PP_2378 0.89 -1.1 2,719,450 + +1.3 2,719,450 + -3.3 2,719,453 + +2.2 2,719,641 + -0.7 2,719,641 + -0.3 2,719,757 + PP_2379 0.17 -2.5 2,719,845 - PP_2379 0.31 -2.3 2,720,022 - PP_2379 0.60 -2.2 2,720,109 - PP_2379 0.75 -1.7 2,720,135 + PP_2379 0.79 -2.2 2,720,159 - PP_2379 0.83 -2.8 2,720,159 - PP_2379 0.83 -1.0 2,720,162 + PP_2379 0.83 -2.1 2,720,162 + PP_2379 0.83 -3.0 2,720,162 + PP_2379 0.83 -1.8 2,720,163 - PP_2379 0.83 -1.9 2,720,163 - PP_2379 0.83 +2.2 2,720,163 - PP_2379 0.83 -3.2 2,720,163 - PP_2379 0.83 -1.8 2,720,163 - PP_2379 0.83 -2.5 2,720,169 - PP_2379 0.84 -2.9 2,720,169 - PP_2379 0.84 -0.6 2,720,304 + -3.0 2,720,305 - +0.6 2,720,305 - -3.5 2,721,055 - -0.6 2,721,145 - +1.0 2,721,149 - -2.1 2,721,149 - -1.1
Or see this region's nucleotide sequence