Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2201 and PP_2202 are separated by 75 nucleotides PP_2202 and PP_2203 overlap by 1 nucleotides PP_2203 and PP_2204 are separated by 14 nucleotides PP_2204 and PP_2205 overlap by 8 nucleotides
PP_2201: PP_2201 - Hydrolase, alpha/beta fold family, at 2,507,175 to 2,508,173
_2201
PP_2202: PP_2202 - conserved membrane protein of unknown function, at 2,508,249 to 2,508,686
_2202
PP_2203: PP_2203 - conserved protein of unknown function, at 2,508,686 to 2,509,114
_2203
PP_2204: PP_2204 - copper resistance protein B, at 2,509,129 to 2,510,013
_2204
PP_2205: PP_2205 - copper resistance protein A, at 2,510,006 to 2,511,730
_2205
Position (kb)
2508
2509
2510 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2507.713 kb on + strand, within PP_2201 at 2507.714 kb on - strand, within PP_2201 at 2507.833 kb on + strand, within PP_2201 at 2508.013 kb on + strand, within PP_2201 at 2508.100 kb on + strand at 2508.100 kb on + strand at 2508.100 kb on + strand at 2508.276 kb on - strand at 2508.276 kb on - strand at 2508.559 kb on - strand, within PP_2202 at 2508.676 kb on - strand at 2508.690 kb on + strand at 2508.690 kb on + strand at 2508.691 kb on - strand at 2508.691 kb on - strand at 2508.691 kb on - strand at 2508.741 kb on + strand, within PP_2203 at 2508.741 kb on + strand, within PP_2203 at 2508.741 kb on + strand, within PP_2203 at 2509.131 kb on - strand at 2509.241 kb on + strand, within PP_2204 at 2509.242 kb on - strand, within PP_2204 at 2509.269 kb on - strand, within PP_2204 at 2509.340 kb on + strand, within PP_2204 at 2509.369 kb on + strand, within PP_2204 at 2509.392 kb on + strand, within PP_2204 at 2509.393 kb on - strand, within PP_2204 at 2509.397 kb on + strand, within PP_2204 at 2509.442 kb on + strand, within PP_2204 at 2509.657 kb on - strand, within PP_2204
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,507,713 + PP_2201 0.54 +0.0 2,507,714 - PP_2201 0.54 +0.3 2,507,833 + PP_2201 0.66 -1.7 2,508,013 + PP_2201 0.84 -0.8 2,508,100 + +0.6 2,508,100 + +0.4 2,508,100 + +0.3 2,508,276 - -1.2 2,508,276 - -0.3 2,508,559 - PP_2202 0.71 +1.0 2,508,676 - -0.6 2,508,690 + +1.1 2,508,690 + -0.7 2,508,691 - -0.3 2,508,691 - -1.2 2,508,691 - +0.0 2,508,741 + PP_2203 0.13 +2.0 2,508,741 + PP_2203 0.13 +1.5 2,508,741 + PP_2203 0.13 +0.5 2,509,131 - +0.9 2,509,241 + PP_2204 0.13 +0.3 2,509,242 - PP_2204 0.13 -0.3 2,509,269 - PP_2204 0.16 -2.7 2,509,340 + PP_2204 0.24 +1.0 2,509,369 + PP_2204 0.27 -0.0 2,509,392 + PP_2204 0.30 +0.9 2,509,393 - PP_2204 0.30 +0.9 2,509,397 + PP_2204 0.30 -0.9 2,509,442 + PP_2204 0.35 -0.5 2,509,657 - PP_2204 0.60 +0.3
Or see this region's nucleotide sequence