Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2131 and PP_2132 are separated by 107 nucleotides PP_2132 and PP_2133 are separated by 83 nucleotides PP_2133 and PP_2134 are separated by 303 nucleotides
PP_2131: PP_2131 - DNA-binding ATPase involved in replication, at 2,432,129 to 2,434,057
_2131
PP_2132: PP_2132 - Universal stress protein, at 2,434,165 to 2,434,605
_2132
PP_2133: PP_2133 - conserved protein of unknown function, at 2,434,689 to 2,435,153
_2133
PP_2134: PP_2134 - transposase, at 2,435,457 to 2,436,422
_2134
Position (kb)
2434
2435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2433.279 kb on - strand, within PP_2131 at 2433.321 kb on + strand, within PP_2131 at 2433.579 kb on - strand, within PP_2131 at 2433.677 kb on + strand, within PP_2131 at 2433.678 kb on - strand, within PP_2131 at 2433.678 kb on - strand, within PP_2131 at 2433.755 kb on - strand, within PP_2131 at 2433.854 kb on + strand, within PP_2131 at 2433.855 kb on - strand, within PP_2131 at 2433.913 kb on + strand at 2433.914 kb on - strand at 2433.953 kb on + strand at 2433.953 kb on + strand at 2433.953 kb on + strand at 2434.023 kb on + strand at 2434.023 kb on + strand at 2434.023 kb on + strand at 2434.024 kb on - strand at 2434.024 kb on - strand at 2434.024 kb on - strand at 2434.453 kb on + strand, within PP_2132 at 2434.453 kb on + strand, within PP_2132 at 2434.453 kb on + strand, within PP_2132 at 2434.454 kb on - strand, within PP_2132 at 2434.504 kb on + strand, within PP_2132 at 2434.598 kb on + strand at 2434.631 kb on - strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.640 kb on - strand at 2434.709 kb on + strand at 2434.709 kb on + strand at 2434.715 kb on + strand at 2435.257 kb on - strand at 2435.257 kb on - strand at 2435.257 kb on - strand at 2435.257 kb on - strand at 2435.257 kb on - strand at 2435.262 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,433,279 - PP_2131 0.60 +1.3 2,433,321 + PP_2131 0.62 -2.4 2,433,579 - PP_2131 0.75 +1.8 2,433,677 + PP_2131 0.80 -0.2 2,433,678 - PP_2131 0.80 -0.4 2,433,678 - PP_2131 0.80 -2.5 2,433,755 - PP_2131 0.84 +1.1 2,433,854 + PP_2131 0.89 -0.5 2,433,855 - PP_2131 0.89 -0.1 2,433,913 + -1.6 2,433,914 - +0.6 2,433,953 + -0.1 2,433,953 + -1.7 2,433,953 + +1.0 2,434,023 + +2.1 2,434,023 + +0.7 2,434,023 + -0.0 2,434,024 - -0.6 2,434,024 - +0.7 2,434,024 - -0.7 2,434,453 + PP_2132 0.65 -2.3 2,434,453 + PP_2132 0.65 +0.6 2,434,453 + PP_2132 0.65 -2.4 2,434,454 - PP_2132 0.66 -1.9 2,434,504 + PP_2132 0.77 -2.0 2,434,598 + -0.0 2,434,631 - +0.5 2,434,639 + -0.1 2,434,639 + -3.1 2,434,639 + -3.2 2,434,639 + +0.3 2,434,639 + -2.1 2,434,640 - +0.9 2,434,709 + +0.1 2,434,709 + +1.8 2,434,715 + -0.5 2,435,257 - -3.3 2,435,257 - -0.3 2,435,257 - +2.4 2,435,257 - +1.8 2,435,257 - -1.3 2,435,262 - +0.3
Or see this region's nucleotide sequence