Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2028 and PP_2029 overlap by 4 nucleotides PP_2029 and PP_2030 overlap by 8 nucleotides PP_2030 and PP_2031 overlap by 4 nucleotides
PP_2028: PP_2028 - conserved protein of unknown function containing tetratricopeptide (TPR) domain repeat, at 2,304,838 to 2,306,556
_2028
PP_2029: PP_2029 - von Willebrand factor type A domain protein, at 2,306,553 to 2,307,629
_2029
PP_2030: PP_2030 - conserved protein of unknown function, at 2,307,622 to 2,308,116
_2030
PP_2031: PP_2031 - conserved protein of unknown function, at 2,308,113 to 2,309,069
_2031
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2305.832 kb on + strand at 2305.833 kb on - strand, within PP_2028 at 2305.961 kb on - strand, within PP_2028 at 2306.141 kb on + strand, within PP_2028 at 2306.141 kb on + strand, within PP_2028 at 2306.141 kb on + strand, within PP_2028 at 2306.142 kb on - strand, within PP_2028 at 2306.413 kb on + strand at 2306.413 kb on + strand at 2306.413 kb on + strand at 2306.414 kb on - strand at 2306.414 kb on - strand at 2306.483 kb on + strand at 2306.484 kb on - strand at 2306.719 kb on + strand, within PP_2029 at 2306.828 kb on - strand, within PP_2029 at 2306.951 kb on - strand, within PP_2029 at 2306.951 kb on - strand, within PP_2029 at 2307.193 kb on + strand at 2307.193 kb on + strand, within PP_2029 at 2307.704 kb on + strand, within PP_2030 at 2307.705 kb on - strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.716 kb on - strand, within PP_2030 at 2307.716 kb on - strand at 2307.722 kb on - strand, within PP_2030 at 2307.722 kb on - strand, within PP_2030 at 2307.887 kb on + strand, within PP_2030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,305,832 + +2.0 2,305,833 - PP_2028 0.58 -4.0 2,305,961 - PP_2028 0.65 -0.3 2,306,141 + PP_2028 0.76 +1.9 2,306,141 + PP_2028 0.76 +0.5 2,306,141 + PP_2028 0.76 +0.3 2,306,142 - PP_2028 0.76 +1.7 2,306,413 + -0.3 2,306,413 + +1.4 2,306,413 + +0.4 2,306,414 - -4.1 2,306,414 - -0.5 2,306,483 + +1.4 2,306,484 - +1.8 2,306,719 + PP_2029 0.15 +1.4 2,306,828 - PP_2029 0.26 +1.4 2,306,951 - PP_2029 0.37 +1.1 2,306,951 - PP_2029 0.37 -0.7 2,307,193 + -0.3 2,307,193 + PP_2029 0.59 -3.0 2,307,704 + PP_2030 0.17 +0.4 2,307,705 - PP_2030 0.17 -1.5 2,307,715 + PP_2030 0.19 -0.7 2,307,715 + PP_2030 0.19 +1.8 2,307,715 + PP_2030 0.19 -1.0 2,307,715 + PP_2030 0.19 -0.4 2,307,715 + PP_2030 0.19 +0.7 2,307,716 - PP_2030 0.19 +0.1 2,307,716 - -1.1 2,307,722 - PP_2030 0.20 +0.5 2,307,722 - PP_2030 0.20 -3.0 2,307,887 + PP_2030 0.54 +0.9
Or see this region's nucleotide sequence