Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5496 and PP_2023 are separated by 132 nucleotides PP_2023 and PP_2024 overlap by 10 nucleotides PP_2024 and PP_2025 overlap by 4 nucleotides
PP_5496: PP_5496 - conserved exported protein of unknown function, at 2,295,578 to 2,295,754
_5496
PP_2023: PP_2023 - Glutathione S-transferase family protein, at 2,295,887 to 2,296,486
_2023
PP_2024: PP_2024 - Exonuclease SbcC, at 2,296,477 to 2,300,121
_2024
PP_2025: PP_2025 - Exonuclease SbcD, at 2,300,118 to 2,301,356
_2025
Position (kb)
2296
2297
2298
2299
2300
2301 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2295.478 kb on + strand at 2295.570 kb on - strand at 2295.602 kb on - strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.687 kb on - strand, within PP_5496 at 2295.699 kb on - strand, within PP_5496 at 2296.057 kb on - strand, within PP_2023 at 2296.158 kb on + strand at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.242 kb on - strand, within PP_2023 at 2296.562 kb on - strand at 2296.864 kb on + strand, within PP_2024 at 2296.864 kb on + strand, within PP_2024 at 2296.864 kb on + strand, within PP_2024 at 2296.865 kb on - strand, within PP_2024 at 2296.865 kb on - strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.905 kb on - strand, within PP_2024 at 2297.599 kb on + strand, within PP_2024 at 2297.791 kb on + strand, within PP_2024 at 2297.792 kb on - strand, within PP_2024 at 2297.795 kb on - strand, within PP_2024 at 2298.077 kb on - strand, within PP_2024 at 2298.662 kb on + strand, within PP_2024 at 2298.788 kb on + strand, within PP_2024 at 2298.988 kb on - strand, within PP_2024 at 2299.447 kb on + strand, within PP_2024 at 2299.447 kb on + strand, within PP_2024 at 2299.683 kb on - strand, within PP_2024 at 2299.727 kb on + strand, within PP_2024 at 2299.727 kb on + strand, within PP_2024 at 2299.728 kb on - strand, within PP_2024 at 2299.728 kb on - strand, within PP_2024 at 2300.630 kb on - strand, within PP_2025 at 2300.689 kb on + strand, within PP_2025 at 2300.689 kb on + strand, within PP_2025 at 2300.696 kb on + strand, within PP_2025 at 2300.885 kb on - strand, within PP_2025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,295,478 + +0.6 2,295,570 - +0.5 2,295,602 - PP_5496 0.14 +0.9 2,295,686 + PP_5496 0.61 -1.2 2,295,686 + PP_5496 0.61 +0.1 2,295,687 - PP_5496 0.62 -0.1 2,295,699 - PP_5496 0.68 -0.6 2,296,057 - PP_2023 0.28 +1.0 2,296,158 + -1.0 2,296,158 + PP_2023 0.45 +0.2 2,296,158 + PP_2023 0.45 +0.2 2,296,158 + PP_2023 0.45 +0.2 2,296,242 - PP_2023 0.59 +0.6 2,296,562 - -1.8 2,296,864 + PP_2024 0.11 -0.0 2,296,864 + PP_2024 0.11 -1.7 2,296,864 + PP_2024 0.11 -1.0 2,296,865 - PP_2024 0.11 +1.0 2,296,865 - PP_2024 0.11 -0.8 2,296,904 + PP_2024 0.12 -1.1 2,296,904 + PP_2024 0.12 -0.6 2,296,904 + PP_2024 0.12 -0.2 2,296,905 - PP_2024 0.12 -0.6 2,297,599 + PP_2024 0.31 +0.6 2,297,791 + PP_2024 0.36 +1.0 2,297,792 - PP_2024 0.36 +0.0 2,297,795 - PP_2024 0.36 +0.2 2,298,077 - PP_2024 0.44 -2.3 2,298,662 + PP_2024 0.60 +0.6 2,298,788 + PP_2024 0.63 -3.7 2,298,988 - PP_2024 0.69 -1.2 2,299,447 + PP_2024 0.81 -0.1 2,299,447 + PP_2024 0.81 -3.2 2,299,683 - PP_2024 0.88 -0.9 2,299,727 + PP_2024 0.89 -4.2 2,299,727 + PP_2024 0.89 -0.1 2,299,728 - PP_2024 0.89 -1.1 2,299,728 - PP_2024 0.89 +0.6 2,300,630 - PP_2025 0.41 +2.3 2,300,689 + PP_2025 0.46 +1.0 2,300,689 + PP_2025 0.46 -0.9 2,300,696 + PP_2025 0.47 +0.4 2,300,885 - PP_2025 0.62 -1.8
Or see this region's nucleotide sequence