Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_t28 and PP_t29 are separated by 61 nucleotides PP_t29 and PP_t30 are separated by 42 nucleotides PP_t30 and PP_1978 are separated by 170 nucleotides PP_1978 and PP_1979 are separated by 15 nucleotides PP_1979 and PP_1980 are separated by 104 nucleotides PP_1980 and PP_1981 are separated by 51 nucleotides
PP_t28: PP_t28 - tRNA-Glu, at 2,241,927 to 2,242,002
_t28
PP_t29: PP_t29 - tRNA-Ala, at 2,242,064 to 2,242,139
_t29
PP_t30: PP_t30 - tRNA-Glu, at 2,242,182 to 2,242,257
_t30
PP_1978: PP_1978 - Transcriptional regulator, TetR family, at 2,242,428 to 2,242,967
_1978
PP_1979: PP_1979 - putative Hydrolase, at 2,242,983 to 2,243,864
_1979
PP_1980: PP_1980 - conserved protein of unknown function, at 2,243,969 to 2,244,403
_1980
PP_1981: PP_1981 - tRNA-dihydrouridine synthase C, at 2,244,455 to 2,245,426
_1981
Position (kb)
2242
2243
2244 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2242.026 kb on + strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand, within PP_t29 at 2242.129 kb on + strand, within PP_t29 at 2242.180 kb on - strand at 2242.284 kb on - strand at 2242.379 kb on - strand at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.561 kb on - strand, within PP_1978 at 2242.561 kb on - strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.695 kb on + strand, within PP_1978 at 2242.695 kb on + strand, within PP_1978 at 2242.696 kb on - strand, within PP_1978 at 2242.726 kb on + strand, within PP_1978 at 2242.726 kb on + strand, within PP_1978 at 2242.727 kb on - strand, within PP_1978 at 2242.988 kb on + strand at 2242.989 kb on - strand at 2242.989 kb on - strand at 2243.139 kb on - strand, within PP_1979 at 2243.139 kb on - strand, within PP_1979 at 2243.264 kb on + strand, within PP_1979 at 2243.265 kb on - strand, within PP_1979 at 2243.265 kb on - strand, within PP_1979 at 2243.798 kb on + strand at 2243.832 kb on - strand at 2243.886 kb on - strand at 2243.887 kb on + strand at 2243.887 kb on + strand at 2243.888 kb on - strand at 2244.510 kb on + strand at 2244.510 kb on + strand at 2244.510 kb on + strand at 2244.510 kb on + strand at 2244.511 kb on - strand at 2244.511 kb on - strand at 2244.511 kb on - strand at 2244.678 kb on + strand, within PP_1981 at 2244.678 kb on + strand, within PP_1981 at 2244.679 kb on - strand, within PP_1981
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,242,026 + +0.7 2,242,072 - PP_t29 0.11 -1.8 2,242,072 - -1.0 2,242,072 - PP_t29 0.11 +0.3 2,242,072 - PP_t29 0.11 +0.6 2,242,129 + PP_t29 0.86 -0.0 2,242,180 - -1.9 2,242,284 - -0.6 2,242,379 - +0.6 2,242,560 + PP_1978 0.24 +0.1 2,242,560 + PP_1978 0.24 +1.1 2,242,560 + PP_1978 0.24 -2.6 2,242,560 + PP_1978 0.24 -1.5 2,242,561 - PP_1978 0.25 -2.0 2,242,561 - PP_1978 0.25 +0.6 2,242,589 + PP_1978 0.30 +0.7 2,242,589 + PP_1978 0.30 +0.9 2,242,589 + PP_1978 0.30 -0.5 2,242,589 + PP_1978 0.30 -0.2 2,242,590 - PP_1978 0.30 +0.4 2,242,590 - PP_1978 0.30 -1.7 2,242,590 - PP_1978 0.30 -1.5 2,242,590 - PP_1978 0.30 +1.1 2,242,695 + PP_1978 0.49 -1.2 2,242,695 + PP_1978 0.49 -3.4 2,242,696 - PP_1978 0.50 -3.1 2,242,726 + PP_1978 0.55 -3.3 2,242,726 + PP_1978 0.55 +0.6 2,242,727 - PP_1978 0.55 +1.3 2,242,988 + -1.0 2,242,989 - -0.3 2,242,989 - -0.6 2,243,139 - PP_1979 0.18 +0.8 2,243,139 - PP_1979 0.18 -0.0 2,243,264 + PP_1979 0.32 +1.7 2,243,265 - PP_1979 0.32 +1.7 2,243,265 - PP_1979 0.32 +0.7 2,243,798 + +0.9 2,243,832 - +0.6 2,243,886 - -1.9 2,243,887 + -0.0 2,243,887 + +0.7 2,243,888 - +0.8 2,244,510 + +0.6 2,244,510 + -0.6 2,244,510 + -1.7 2,244,510 + +0.9 2,244,511 - -2.3 2,244,511 - -2.8 2,244,511 - +0.4 2,244,678 + PP_1981 0.23 +1.2 2,244,678 + PP_1981 0.23 +0.5 2,244,679 - PP_1981 0.23 -0.0
Or see this region's nucleotide sequence