Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1109 and PP_1110 are separated by 181 nucleotides PP_1110 and PP_1111 overlap by 4 nucleotides PP_1111 and PP_1112 overlap by 4 nucleotides
PP_1109: PP_1109 - Transcriptional regulator, GntR family, at 1,268,091 to 1,269,431
_1109
PP_1110: PP_1110 - serine acetyltransferase, at 1,269,613 to 1,270,317
_1110
PP_1111: PP_1111 - putative Synthetase, at 1,270,314 to 1,271,102
_1111
PP_1112: PP_1112 - conserved membrane protein of unknown function, at 1,271,099 to 1,272,310
_1112
Position (kb)
1269
1270
1271 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1268.615 kb on - strand, within PP_1109 at 1269.019 kb on + strand, within PP_1109 at 1269.020 kb on - strand, within PP_1109 at 1269.244 kb on + strand, within PP_1109 at 1269.245 kb on - strand, within PP_1109 at 1269.759 kb on - strand, within PP_1110 at 1269.862 kb on + strand at 1269.902 kb on - strand, within PP_1110 at 1269.910 kb on - strand, within PP_1110 at 1270.084 kb on + strand, within PP_1110 at 1270.085 kb on - strand, within PP_1110 at 1270.249 kb on - strand at 1270.329 kb on + strand at 1270.363 kb on - strand at 1270.437 kb on - strand, within PP_1111 at 1270.544 kb on + strand, within PP_1111 at 1270.590 kb on - strand, within PP_1111 at 1270.590 kb on - strand, within PP_1111 at 1270.661 kb on + strand, within PP_1111 at 1270.719 kb on + strand, within PP_1111 at 1270.719 kb on + strand, within PP_1111 at 1270.775 kb on + strand, within PP_1111 at 1270.897 kb on - strand, within PP_1111 at 1270.897 kb on - strand, within PP_1111 at 1270.935 kb on + strand, within PP_1111 at 1270.935 kb on + strand, within PP_1111 at 1270.936 kb on - strand, within PP_1111 at 1270.936 kb on - strand, within PP_1111 at 1270.936 kb on - strand, within PP_1111 at 1270.936 kb on - strand, within PP_1111 at 1270.983 kb on - strand, within PP_1111 at 1270.983 kb on - strand, within PP_1111 at 1271.024 kb on + strand at 1271.083 kb on - strand at 1271.083 kb on - strand at 1271.099 kb on - strand at 1271.099 kb on - strand at 1271.099 kb on - strand at 1271.099 kb on - strand at 1271.126 kb on + strand at 1271.127 kb on - strand at 1271.132 kb on + strand at 1271.132 kb on + strand at 1271.133 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 1,268,615 - PP_1109 0.39 +0.6 1,269,019 + PP_1109 0.69 +0.4 1,269,020 - PP_1109 0.69 +0.4 1,269,244 + PP_1109 0.86 +1.4 1,269,245 - PP_1109 0.86 +1.0 1,269,759 - PP_1110 0.21 +0.8 1,269,862 + -1.0 1,269,902 - PP_1110 0.41 +1.4 1,269,910 - PP_1110 0.42 +1.0 1,270,084 + PP_1110 0.67 +1.3 1,270,085 - PP_1110 0.67 +0.6 1,270,249 - +0.1 1,270,329 + +1.4 1,270,363 - +0.2 1,270,437 - PP_1111 0.16 +0.7 1,270,544 + PP_1111 0.29 +0.4 1,270,590 - PP_1111 0.35 +1.1 1,270,590 - PP_1111 0.35 -0.3 1,270,661 + PP_1111 0.44 +0.5 1,270,719 + PP_1111 0.51 -3.1 1,270,719 + PP_1111 0.51 +0.3 1,270,775 + PP_1111 0.58 -2.5 1,270,897 - PP_1111 0.74 +1.0 1,270,897 - PP_1111 0.74 +1.3 1,270,935 + PP_1111 0.79 +1.0 1,270,935 + PP_1111 0.79 -2.8 1,270,936 - PP_1111 0.79 +1.5 1,270,936 - PP_1111 0.79 +0.0 1,270,936 - PP_1111 0.79 -0.1 1,270,936 - PP_1111 0.79 -0.5 1,270,983 - PP_1111 0.85 +1.0 1,270,983 - PP_1111 0.85 +1.6 1,271,024 + +2.5 1,271,083 - +0.9 1,271,083 - +1.1 1,271,099 - +0.6 1,271,099 - +1.2 1,271,099 - -1.8 1,271,099 - -0.1 1,271,126 + -0.5 1,271,127 - +0.0 1,271,132 + -0.0 1,271,132 + -1.9 1,271,133 - +0.7
Or see this region's nucleotide sequence