Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0751 and PP_5456 are separated by 601 nucleotides PP_5456 and PP_0753 are separated by 145 nucleotides PP_0753 and PP_0754 are separated by 74 nucleotides PP_0754 and PP_0755 are separated by 67 nucleotides PP_0755 and PP_0756 are separated by 35 nucleotides
PP_0751: PP_0751 - malate:quinone oxidoreductase, at 868,079 to 869,587
_0751
PP_5456: PP_5456 - conserved protein of unknown function, at 870,189 to 870,422
_5456
PP_0753: PP_0753 - putative Lipoprotein, at 870,568 to 871,152
_0753
PP_0754: PP_0754 - conserved protein of unknown function, at 871,227 to 871,538
_0754
PP_0755: PP_0755 - conserved protein of unknown function, at 871,606 to 871,854
_0755
PP_0756: PP_0756 - conserved protein of unknown function, at 871,890 to 873,161
_0756
Position (kb) 870
871
872 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 869.608 kb on - strand at 869.608 kb on - strand at 869.618 kb on - strand at 869.618 kb on - strand at 869.618 kb on - strand at 869.618 kb on - strand at 869.618 kb on - strand at 869.618 kb on - strand at 869.630 kb on - strand at 869.630 kb on - strand at 869.630 kb on - strand at 869.630 kb on - strand at 869.630 kb on - strand at 869.630 kb on - strand at 869.630 kb on - strand at 869.758 kb on - strand at 869.805 kb on - strand at 870.117 kb on - strand at 870.117 kb on - strand at 870.119 kb on - strand at 870.134 kb on - strand at 870.134 kb on - strand at 870.136 kb on - strand at 870.136 kb on - strand at 870.136 kb on - strand at 870.136 kb on - strand at 870.176 kb on - strand at 870.374 kb on - strand, within PP_5456 at 870.374 kb on - strand, within PP_5456 at 870.461 kb on - strand at 870.524 kb on + strand at 870.524 kb on + strand at 870.528 kb on + strand at 870.528 kb on + strand at 870.528 kb on + strand at 870.528 kb on + strand at 870.528 kb on + strand at 870.528 kb on + strand at 870.528 kb on + strand at 870.528 kb on + strand at 870.529 kb on - strand at 870.529 kb on - strand at 870.529 kb on - strand at 870.529 kb on - strand at 870.529 kb on - strand at 870.529 kb on - strand at 870.529 kb on - strand at 870.529 kb on - strand at 870.530 kb on + strand at 870.530 kb on + strand at 870.530 kb on + strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.531 kb on - strand at 870.763 kb on + strand, within PP_0753 at 870.764 kb on - strand, within PP_0753 at 870.764 kb on - strand, within PP_0753 at 870.764 kb on - strand, within PP_0753 at 870.916 kb on + strand, within PP_0753 at 870.916 kb on + strand at 870.917 kb on - strand, within PP_0753 at 870.917 kb on - strand, within PP_0753 at 870.917 kb on - strand, within PP_0753 at 870.940 kb on + strand, within PP_0753 at 870.940 kb on + strand, within PP_0753 at 870.941 kb on - strand, within PP_0753 at 870.941 kb on - strand, within PP_0753 at 870.941 kb on - strand, within PP_0753 at 870.941 kb on - strand, within PP_0753 at 870.955 kb on - strand, within PP_0753 at 871.003 kb on + strand, within PP_0753 at 871.150 kb on + strand at 871.151 kb on - strand at 871.151 kb on - strand at 871.151 kb on - strand at 871.228 kb on + strand at 871.228 kb on + strand at 871.228 kb on + strand at 871.239 kb on + strand at 871.239 kb on + strand at 871.239 kb on + strand at 871.239 kb on + strand at 871.240 kb on - strand at 871.240 kb on - strand at 871.240 kb on - strand at 871.321 kb on + strand, within PP_0754 at 871.322 kb on - strand, within PP_0754 at 871.322 kb on - strand, within PP_0754 at 871.322 kb on - strand, within PP_0754 at 871.322 kb on - strand, within PP_0754 at 871.402 kb on + strand, within PP_0754 at 871.402 kb on + strand, within PP_0754 at 871.403 kb on - strand, within PP_0754 at 871.475 kb on + strand, within PP_0754 at 871.475 kb on + strand, within PP_0754 at 871.552 kb on + strand at 871.552 kb on + strand at 871.553 kb on - strand at 871.553 kb on - strand at 871.729 kb on + strand, within PP_0755 at 871.730 kb on - strand, within PP_0755 at 871.730 kb on - strand, within PP_0755 at 871.740 kb on - strand, within PP_0755 at 871.740 kb on - strand, within PP_0755 at 871.783 kb on + strand, within PP_0755 at 871.783 kb on + strand, within PP_0755 at 871.783 kb on + strand, within PP_0755 at 871.784 kb on - strand, within PP_0755 at 871.789 kb on + strand, within PP_0755 at 871.789 kb on + strand, within PP_0755 at 871.790 kb on - strand, within PP_0755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 869,608 - +1.4 869,608 - +1.1 869,618 - +0.9 869,618 - +0.1 869,618 - +1.6 869,618 - +0.2 869,618 - -0.2 869,618 - -0.2 869,630 - -2.6 869,630 - -0.6 869,630 - -2.0 869,630 - +1.0 869,630 - -0.0 869,630 - -0.4 869,630 - +1.1 869,758 - -0.3 869,805 - +0.3 870,117 - +0.0 870,117 - +0.8 870,119 - +1.4 870,134 - -0.1 870,134 - +0.7 870,136 - -0.2 870,136 - -3.6 870,136 - +0.9 870,136 - -0.9 870,176 - -2.4 870,374 - PP_5456 0.79 +1.2 870,374 - PP_5456 0.79 +0.8 870,461 - +0.3 870,524 + +1.1 870,524 + +0.1 870,528 + -3.4 870,528 + +0.6 870,528 + -1.1 870,528 + -2.2 870,528 + +1.8 870,528 + -3.7 870,528 + +1.2 870,528 + +0.1 870,529 - +0.7 870,529 - +1.0 870,529 - +0.1 870,529 - +1.6 870,529 - -1.4 870,529 - +1.4 870,529 - -0.7 870,529 - -1.0 870,530 + +0.7 870,530 + +1.3 870,530 + +0.0 870,531 - +1.0 870,531 - -0.4 870,531 - -0.7 870,531 - -1.2 870,531 - -0.4 870,531 - +1.7 870,531 - +1.6 870,531 - +0.8 870,531 - +0.9 870,531 - -2.5 870,531 - -1.2 870,531 - +0.9 870,531 - +3.3 870,531 - -1.9 870,763 + PP_0753 0.33 +1.5 870,764 - PP_0753 0.34 +0.0 870,764 - PP_0753 0.34 +1.5 870,764 - PP_0753 0.34 +0.5 870,916 + PP_0753 0.59 +1.7 870,916 + -0.6 870,917 - PP_0753 0.60 +2.1 870,917 - PP_0753 0.60 +0.5 870,917 - PP_0753 0.60 -0.2 870,940 + PP_0753 0.64 +0.2 870,940 + PP_0753 0.64 -0.1 870,941 - PP_0753 0.64 +0.4 870,941 - PP_0753 0.64 +0.7 870,941 - PP_0753 0.64 -0.5 870,941 - PP_0753 0.64 -0.0 870,955 - PP_0753 0.66 +1.0 871,003 + PP_0753 0.74 +0.9 871,150 + +1.6 871,151 - +1.0 871,151 - +0.8 871,151 - +0.4 871,228 + +0.2 871,228 + -1.4 871,228 + -2.7 871,239 + -0.9 871,239 + -0.1 871,239 + +1.4 871,239 + +0.9 871,240 - +0.6 871,240 - -0.8 871,240 - +0.7 871,321 + PP_0754 0.30 +0.1 871,322 - PP_0754 0.30 +0.7 871,322 - PP_0754 0.30 -1.8 871,322 - PP_0754 0.30 -0.2 871,322 - PP_0754 0.30 +0.2 871,402 + PP_0754 0.56 +2.3 871,402 + PP_0754 0.56 -1.0 871,403 - PP_0754 0.56 -0.9 871,475 + PP_0754 0.79 -3.8 871,475 + PP_0754 0.79 -1.8 871,552 + -2.8 871,552 + +0.4 871,553 - -0.8 871,553 - -0.9 871,729 + PP_0755 0.49 +0.1 871,730 - PP_0755 0.50 -0.5 871,730 - PP_0755 0.50 +0.2 871,740 - PP_0755 0.54 -0.4 871,740 - PP_0755 0.54 -3.6 871,783 + PP_0755 0.71 -0.2 871,783 + PP_0755 0.71 +1.5 871,783 + PP_0755 0.71 -0.7 871,784 - PP_0755 0.71 +1.3 871,789 + PP_0755 0.73 +0.1 871,789 + PP_0755 0.73 +0.2 871,790 - PP_0755 0.74 +0.7
Or see this region's nucleotide sequence