Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0736 and PP_0737 are separated by 104 nucleotides PP_0737 and PP_0738 are separated by 115 nucleotides PP_0738 and PP_0739 overlap by 4 nucleotides
PP_0736: PP_0736 - Glutamate racemase, at 855,150 to 855,947
_0736
PP_0737: PP_0737 - lipid A 3-O-deacylase, at 856,052 to 856,570
_0737
PP_0738: PP_0738 - conserved protein of unknown function, at 856,686 to 857,384
_0738
PP_0739: PP_0739 - deoxyribodipyrimidine photolyase, at 857,381 to 858,823
_0739
Position (kb) 856
857
858 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.972 kb on + strand at 855.973 kb on - strand at 855.994 kb on - strand at 855.994 kb on - strand at 856.023 kb on + strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.052 kb on - strand at 856.253 kb on + strand, within PP_0737 at 856.253 kb on + strand, within PP_0737 at 856.254 kb on - strand, within PP_0737 at 856.259 kb on + strand, within PP_0737 at 856.259 kb on + strand, within PP_0737 at 856.259 kb on + strand, within PP_0737 at 856.341 kb on + strand, within PP_0737 at 856.350 kb on - strand, within PP_0737 at 856.350 kb on - strand, within PP_0737 at 856.533 kb on + strand at 856.533 kb on + strand at 856.534 kb on - strand at 856.534 kb on - strand at 856.553 kb on + strand at 856.554 kb on - strand at 856.611 kb on + strand at 856.612 kb on - strand at 856.622 kb on + strand at 856.623 kb on - strand at 856.623 kb on - strand at 856.634 kb on + strand at 856.635 kb on - strand at 856.668 kb on + strand at 856.668 kb on + strand at 856.668 kb on + strand at 856.668 kb on + strand at 856.669 kb on - strand at 856.669 kb on - strand at 856.709 kb on + strand at 856.709 kb on + strand at 856.709 kb on + strand at 856.710 kb on - strand at 856.710 kb on - strand at 856.710 kb on - strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.713 kb on - strand at 856.852 kb on - strand, within PP_0738 at 856.889 kb on + strand, within PP_0738 at 857.113 kb on + strand, within PP_0738 at 857.113 kb on + strand, within PP_0738 at 857.114 kb on - strand, within PP_0738 at 857.358 kb on - strand at 857.369 kb on + strand at 857.378 kb on + strand at 857.390 kb on + strand at 857.462 kb on + strand at 857.462 kb on + strand at 857.462 kb on + strand at 857.462 kb on + strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.474 kb on + strand at 857.474 kb on + strand at 857.474 kb on + strand at 857.475 kb on - strand at 857.478 kb on + strand at 857.479 kb on - strand at 857.485 kb on + strand at 857.486 kb on - strand at 857.489 kb on - strand at 857.509 kb on + strand at 857.509 kb on + strand at 857.546 kb on + strand, within PP_0739 at 857.628 kb on + strand, within PP_0739 at 857.628 kb on + strand, within PP_0739 at 857.628 kb on + strand, within PP_0739 at 857.971 kb on - strand, within PP_0739 at 857.988 kb on - strand, within PP_0739 at 858.118 kb on + strand, within PP_0739 at 858.119 kb on - strand, within PP_0739 at 858.260 kb on + strand, within PP_0739
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 855,915 - -0.2 855,915 - -3.6 855,915 - +0.7 855,915 - +0.0 855,972 + +0.6 855,973 - -2.8 855,994 - +1.0 855,994 - +0.3 856,023 + -0.4 856,024 - -0.2 856,024 - -1.4 856,024 - +1.1 856,052 - +0.7 856,253 + PP_0737 0.39 +0.4 856,253 + PP_0737 0.39 +0.6 856,254 - PP_0737 0.39 +0.8 856,259 + PP_0737 0.40 +0.3 856,259 + PP_0737 0.40 +0.9 856,259 + PP_0737 0.40 -0.9 856,341 + PP_0737 0.56 +1.1 856,350 - PP_0737 0.57 -0.2 856,350 - PP_0737 0.57 +0.1 856,533 + +1.0 856,533 + -2.9 856,534 - +0.3 856,534 - -2.3 856,553 + +1.8 856,554 - +1.0 856,611 + +0.5 856,612 - -0.1 856,622 + +1.3 856,623 - +0.0 856,623 - -1.0 856,634 + -0.4 856,635 - +0.5 856,668 + +0.8 856,668 + -0.2 856,668 + -0.9 856,668 + +0.4 856,669 - +1.2 856,669 - -0.0 856,709 + -2.0 856,709 + -3.6 856,709 + -0.9 856,710 - +1.2 856,710 - +1.4 856,710 - -3.3 856,712 + +0.4 856,712 + -0.1 856,712 + -0.4 856,712 + -0.2 856,712 + +0.6 856,712 + -2.7 856,713 - -2.2 856,852 - PP_0738 0.24 -0.3 856,889 + PP_0738 0.29 -1.5 857,113 + PP_0738 0.61 -0.3 857,113 + PP_0738 0.61 -3.2 857,114 - PP_0738 0.61 -3.2 857,358 - -2.1 857,369 + +0.0 857,378 + +1.1 857,390 + -4.2 857,462 + +0.4 857,462 + +0.5 857,462 + -0.4 857,462 + -3.8 857,463 - +0.6 857,463 - -0.4 857,463 - +0.1 857,463 - +1.2 857,463 - -0.2 857,474 + +0.8 857,474 + +1.0 857,474 + -1.9 857,475 - -0.2 857,478 + +0.3 857,479 - +0.4 857,485 + +0.8 857,486 - +0.2 857,489 - +1.4 857,509 + +0.3 857,509 + -1.4 857,546 + PP_0739 0.11 -0.9 857,628 + PP_0739 0.17 -1.8 857,628 + PP_0739 0.17 +0.0 857,628 + PP_0739 0.17 +0.5 857,971 - PP_0739 0.41 -2.1 857,988 - PP_0739 0.42 -0.5 858,118 + PP_0739 0.51 +0.8 858,119 - PP_0739 0.51 -0.2 858,260 + PP_0739 0.61 +0.8
Or see this region's nucleotide sequence