Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0672 and PP_0673 are separated by 221 nucleotides PP_0673 and PP_0674 are separated by 304 nucleotides PP_0674 and PP_0675 are separated by 212 nucleotides
PP_0672: PP_0672 - Sensory box protein, at 782,095 to 785,925
_0672
PP_0673: PP_0673 - conserved protein of unknown function, at 786,147 to 786,578
_0673
PP_0674: PP_0674 - ADP/ATP ratio sensor and inhibitor of translation, at 786,883 to 788,550
_0674
PP_0675: PP_0675 - glutamate dehydrogenase, NADP-dependent, at 788,763 to 790,112
_0675
Position (kb) 786
787
788
789 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 785.913 kb on + strand at 785.913 kb on + strand at 785.913 kb on + strand at 785.913 kb on + strand at 786.015 kb on + strand at 786.015 kb on + strand at 786.015 kb on + strand at 786.015 kb on + strand at 786.016 kb on - strand at 786.016 kb on - strand at 786.016 kb on - strand at 786.017 kb on + strand at 786.017 kb on + strand at 786.017 kb on + strand at 786.017 kb on + strand at 786.018 kb on - strand at 786.018 kb on - strand at 786.018 kb on - strand at 786.018 kb on - strand at 786.018 kb on - strand at 786.042 kb on - strand at 786.042 kb on - strand at 786.056 kb on + strand at 786.262 kb on + strand, within PP_0673 at 786.263 kb on - strand, within PP_0673 at 786.263 kb on - strand, within PP_0673 at 786.754 kb on + strand at 786.754 kb on + strand at 786.754 kb on + strand at 786.754 kb on + strand at 786.755 kb on - strand at 786.755 kb on - strand at 786.755 kb on - strand at 786.755 kb on - strand at 786.809 kb on - strand at 786.857 kb on + strand at 786.857 kb on + strand at 786.858 kb on - strand at 786.858 kb on - strand at 786.858 kb on - strand at 786.861 kb on + strand at 786.862 kb on - strand at 786.869 kb on + strand at 786.892 kb on + strand at 786.893 kb on - strand at 786.893 kb on - strand at 786.893 kb on - strand at 786.893 kb on - strand at 786.893 kb on - strand at 786.898 kb on + strand at 786.898 kb on + strand at 786.898 kb on + strand at 786.898 kb on + strand at 786.899 kb on - strand at 786.899 kb on - strand at 786.993 kb on - strand at 787.099 kb on + strand, within PP_0674 at 787.100 kb on - strand, within PP_0674 at 787.169 kb on + strand, within PP_0674 at 787.204 kb on + strand, within PP_0674 at 787.204 kb on + strand, within PP_0674 at 787.205 kb on - strand, within PP_0674 at 787.214 kb on - strand, within PP_0674 at 787.540 kb on + strand, within PP_0674 at 787.540 kb on + strand, within PP_0674 at 787.541 kb on - strand, within PP_0674 at 787.612 kb on + strand at 787.816 kb on + strand, within PP_0674 at 787.816 kb on + strand, within PP_0674 at 787.816 kb on + strand, within PP_0674 at 787.816 kb on + strand, within PP_0674 at 787.879 kb on + strand, within PP_0674 at 787.965 kb on + strand, within PP_0674 at 787.966 kb on - strand, within PP_0674 at 788.039 kb on + strand, within PP_0674 at 788.039 kb on + strand, within PP_0674 at 788.140 kb on + strand, within PP_0674 at 788.141 kb on - strand, within PP_0674 at 788.141 kb on - strand, within PP_0674 at 788.141 kb on - strand, within PP_0674 at 788.157 kb on + strand, within PP_0674 at 788.157 kb on + strand, within PP_0674 at 788.161 kb on + strand, within PP_0674 at 788.161 kb on + strand, within PP_0674 at 788.161 kb on + strand, within PP_0674 at 788.161 kb on + strand, within PP_0674 at 788.161 kb on + strand, within PP_0674 at 788.161 kb on + strand, within PP_0674 at 788.161 kb on + strand, within PP_0674 at 788.162 kb on - strand, within PP_0674 at 788.162 kb on - strand, within PP_0674 at 788.162 kb on - strand, within PP_0674 at 788.162 kb on - strand, within PP_0674 at 788.162 kb on - strand, within PP_0674 at 788.457 kb on + strand at 788.458 kb on - strand at 788.461 kb on + strand at 788.461 kb on + strand at 788.461 kb on + strand at 788.461 kb on + strand at 788.462 kb on - strand at 788.462 kb on - strand at 788.462 kb on - strand at 788.462 kb on - strand at 788.525 kb on + strand at 788.618 kb on - strand at 788.653 kb on - strand at 788.721 kb on + strand at 788.721 kb on + strand at 788.722 kb on - strand at 788.722 kb on - strand at 788.742 kb on - strand at 788.898 kb on + strand, within PP_0675 at 788.898 kb on + strand, within PP_0675 at 789.036 kb on + strand, within PP_0675 at 789.036 kb on + strand, within PP_0675 at 789.228 kb on + strand, within PP_0675 at 789.256 kb on + strand, within PP_0675 at 789.257 kb on - strand, within PP_0675 at 789.313 kb on - strand, within PP_0675 at 789.313 kb on - strand, within PP_0675 at 789.313 kb on - strand, within PP_0675 at 789.367 kb on - strand, within PP_0675 at 789.414 kb on + strand, within PP_0675 at 789.415 kb on - strand, within PP_0675 at 789.452 kb on + strand, within PP_0675 at 789.501 kb on + strand, within PP_0675 at 789.502 kb on - strand, within PP_0675 at 789.502 kb on - strand, within PP_0675 at 789.502 kb on - strand, within PP_0675 at 789.512 kb on + strand, within PP_0675 at 789.512 kb on + strand, within PP_0675 at 789.513 kb on - strand, within PP_0675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 785,913 + -2.2 785,913 + -2.9 785,913 + -2.1 785,913 + -1.5 786,015 + -0.2 786,015 + +0.8 786,015 + +0.4 786,015 + -0.2 786,016 - +0.7 786,016 - +0.9 786,016 - -3.1 786,017 + +0.7 786,017 + -2.9 786,017 + -1.2 786,017 + -1.2 786,018 - -1.7 786,018 - +0.8 786,018 - +1.6 786,018 - +1.3 786,018 - +0.2 786,042 - +0.6 786,042 - -0.6 786,056 + -0.6 786,262 + PP_0673 0.27 -0.5 786,263 - PP_0673 0.27 -1.4 786,263 - PP_0673 0.27 +0.7 786,754 + -2.0 786,754 + -0.8 786,754 + +1.1 786,754 + +0.6 786,755 - +3.5 786,755 - -0.6 786,755 - +1.3 786,755 - +1.0 786,809 - +1.5 786,857 + -2.5 786,857 + +2.1 786,858 - -0.8 786,858 - +1.6 786,858 - -2.1 786,861 + +0.3 786,862 - +1.1 786,869 + +0.5 786,892 + +0.7 786,893 - -1.5 786,893 - -1.0 786,893 - +1.0 786,893 - +1.2 786,893 - +0.8 786,898 + +2.3 786,898 + -0.4 786,898 + +0.5 786,898 + +0.3 786,899 - -0.1 786,899 - -0.5 786,993 - -3.6 787,099 + PP_0674 0.13 +0.1 787,100 - PP_0674 0.13 +1.1 787,169 + PP_0674 0.17 +1.5 787,204 + PP_0674 0.19 +0.4 787,204 + PP_0674 0.19 +0.2 787,205 - PP_0674 0.19 +1.5 787,214 - PP_0674 0.20 -0.3 787,540 + PP_0674 0.39 +1.1 787,540 + PP_0674 0.39 -0.0 787,541 - PP_0674 0.39 -2.2 787,612 + +0.3 787,816 + PP_0674 0.56 +0.2 787,816 + PP_0674 0.56 -2.1 787,816 + PP_0674 0.56 +0.3 787,816 + PP_0674 0.56 -1.6 787,879 + PP_0674 0.60 +1.5 787,965 + PP_0674 0.65 +0.9 787,966 - PP_0674 0.65 +0.4 788,039 + PP_0674 0.69 +0.6 788,039 + PP_0674 0.69 +1.1 788,140 + PP_0674 0.75 +0.4 788,141 - PP_0674 0.75 +1.0 788,141 - PP_0674 0.75 -0.1 788,141 - PP_0674 0.75 +0.8 788,157 + PP_0674 0.76 +0.3 788,157 + PP_0674 0.76 -0.7 788,161 + PP_0674 0.77 +2.5 788,161 + PP_0674 0.77 +0.1 788,161 + PP_0674 0.77 +0.9 788,161 + PP_0674 0.77 +1.2 788,161 + PP_0674 0.77 +2.5 788,161 + PP_0674 0.77 +1.3 788,161 + PP_0674 0.77 -1.7 788,162 - PP_0674 0.77 -1.6 788,162 - PP_0674 0.77 +0.4 788,162 - PP_0674 0.77 +0.4 788,162 - PP_0674 0.77 -0.3 788,162 - PP_0674 0.77 +1.1 788,457 + +0.3 788,458 - -0.8 788,461 + -0.0 788,461 + -0.9 788,461 + +1.0 788,461 + -2.9 788,462 - +0.5 788,462 - +0.5 788,462 - -1.1 788,462 - +0.7 788,525 + +1.0 788,618 - -2.9 788,653 - -0.2 788,721 + +1.2 788,721 + +1.1 788,722 - -1.4 788,722 - -0.8 788,742 - +0.7 788,898 + PP_0675 0.10 +1.2 788,898 + PP_0675 0.10 -2.9 789,036 + PP_0675 0.20 -2.3 789,036 + PP_0675 0.20 +1.6 789,228 + PP_0675 0.34 +0.5 789,256 + PP_0675 0.37 -0.1 789,257 - PP_0675 0.37 -2.5 789,313 - PP_0675 0.41 +0.7 789,313 - PP_0675 0.41 +0.3 789,313 - PP_0675 0.41 +0.9 789,367 - PP_0675 0.45 -1.9 789,414 + PP_0675 0.48 -1.3 789,415 - PP_0675 0.48 -1.0 789,452 + PP_0675 0.51 -0.8 789,501 + PP_0675 0.55 +0.2 789,502 - PP_0675 0.55 +0.1 789,502 - PP_0675 0.55 -1.1 789,502 - PP_0675 0.55 -1.3 789,512 + PP_0675 0.55 -3.0 789,512 + PP_0675 0.55 -4.4 789,513 - PP_0675 0.56 +1.3
Or see this region's nucleotide sequence