Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0434 and PP_0435 are separated by 3 nucleotides PP_0435 and PP_0436 are separated by 207 nucleotides
PP_0434: PP_0434 - Anhydro-N-acetylmuramic acid kinase, at 520,204 to 521,295
_0434
PP_0435: PP_0435 - Peptidase, M23/M37 family, at 521,299 to 522,726
_0435
PP_0436: PP_0436 - Tyrosine--tRNA ligase, at 522,934 to 524,133
_0436
Position (kb) 521
522
523 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 520.337 kb on - strand, within PP_0434 at 520.343 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.376 kb on - strand, within PP_0434 at 520.692 kb on - strand, within PP_0434 at 520.692 kb on - strand, within PP_0434 at 520.692 kb on - strand, within PP_0434 at 520.807 kb on + strand, within PP_0434 at 520.807 kb on + strand, within PP_0434 at 520.808 kb on - strand, within PP_0434 at 520.813 kb on - strand, within PP_0434 at 520.978 kb on - strand, within PP_0434 at 521.076 kb on + strand, within PP_0434 at 521.077 kb on - strand, within PP_0434 at 521.195 kb on + strand at 521.196 kb on - strand at 521.285 kb on + strand at 521.285 kb on + strand at 521.285 kb on + strand at 521.286 kb on - strand at 521.301 kb on - strand at 521.466 kb on - strand, within PP_0435 at 521.507 kb on + strand, within PP_0435 at 521.507 kb on + strand, within PP_0435 at 521.508 kb on - strand, within PP_0435 at 521.580 kb on - strand, within PP_0435 at 521.591 kb on + strand, within PP_0435 at 521.592 kb on - strand, within PP_0435 at 521.837 kb on + strand, within PP_0435 at 521.837 kb on + strand, within PP_0435 at 521.837 kb on + strand, within PP_0435 at 521.838 kb on - strand, within PP_0435 at 521.838 kb on - strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.882 kb on + strand, within PP_0435 at 521.883 kb on - strand, within PP_0435 at 521.883 kb on - strand, within PP_0435 at 521.963 kb on + strand, within PP_0435 at 521.964 kb on - strand, within PP_0435 at 522.017 kb on + strand, within PP_0435 at 522.113 kb on + strand, within PP_0435 at 522.113 kb on + strand, within PP_0435 at 522.125 kb on - strand, within PP_0435 at 522.159 kb on - strand, within PP_0435 at 522.367 kb on - strand, within PP_0435 at 522.622 kb on + strand at 522.623 kb on - strand at 522.686 kb on + strand at 522.687 kb on - strand at 522.789 kb on + strand at 522.873 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 520,337 - PP_0434 0.12 -0.9 520,343 + PP_0434 0.13 -0.3 520,375 + PP_0434 0.16 -1.1 520,375 + PP_0434 0.16 +0.8 520,376 - PP_0434 0.16 +1.0 520,692 - PP_0434 0.45 -0.7 520,692 - PP_0434 0.45 -1.3 520,692 - PP_0434 0.45 -0.9 520,807 + PP_0434 0.55 +0.1 520,807 + PP_0434 0.55 +0.1 520,808 - PP_0434 0.55 -3.2 520,813 - PP_0434 0.56 +1.1 520,978 - PP_0434 0.71 -1.8 521,076 + PP_0434 0.80 +0.6 521,077 - PP_0434 0.80 +0.5 521,195 + -0.9 521,196 - -1.5 521,285 + +1.5 521,285 + -1.9 521,285 + +1.5 521,286 - +0.9 521,301 - -2.0 521,466 - PP_0435 0.12 -0.7 521,507 + PP_0435 0.15 -2.8 521,507 + PP_0435 0.15 +4.5 521,508 - PP_0435 0.15 +1.4 521,580 - PP_0435 0.20 +1.1 521,591 + PP_0435 0.20 +1.4 521,592 - PP_0435 0.21 -0.0 521,837 + PP_0435 0.38 -2.1 521,837 + PP_0435 0.38 +1.0 521,837 + PP_0435 0.38 -2.3 521,838 - PP_0435 0.38 -0.6 521,838 - PP_0435 0.38 +0.2 521,840 + PP_0435 0.38 -0.3 521,840 + PP_0435 0.38 -1.1 521,840 + PP_0435 0.38 +1.7 521,840 + PP_0435 0.38 +0.9 521,840 + PP_0435 0.38 +3.9 521,841 - PP_0435 0.38 -0.8 521,841 - PP_0435 0.38 -0.2 521,841 - +1.3 521,841 - PP_0435 0.38 -2.0 521,841 - PP_0435 0.38 +1.2 521,841 - PP_0435 0.38 +0.7 521,841 - +2.9 521,844 + PP_0435 0.38 -1.1 521,844 + PP_0435 0.38 +0.9 521,844 + PP_0435 0.38 -2.7 521,844 + PP_0435 0.38 +0.6 521,844 + PP_0435 0.38 -1.7 521,844 + PP_0435 0.38 +0.3 521,845 - PP_0435 0.38 +0.5 521,845 - PP_0435 0.38 +0.7 521,845 - PP_0435 0.38 +0.5 521,845 - PP_0435 0.38 +1.6 521,845 - PP_0435 0.38 +1.7 521,882 + PP_0435 0.41 -0.0 521,883 - PP_0435 0.41 +0.5 521,883 - PP_0435 0.41 +0.4 521,963 + PP_0435 0.46 +0.8 521,964 - PP_0435 0.47 +2.2 522,017 + PP_0435 0.50 +2.8 522,113 + PP_0435 0.57 +1.5 522,113 + PP_0435 0.57 -1.3 522,125 - PP_0435 0.58 -0.6 522,159 - PP_0435 0.60 +0.4 522,367 - PP_0435 0.75 +1.0 522,622 + +2.0 522,623 - -3.5 522,686 + +1.5 522,687 - +0.4 522,789 + -0.2 522,873 + -0.8
Or see this region's nucleotide sequence