Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0409 and PP_0410 are separated by 10 nucleotides PP_0410 and PP_0411 are separated by 295 nucleotides PP_0411 and PP_0412 are separated by 40 nucleotides
PP_0409: PP_0409 - Sensory box histidine kinase, at 496,129 to 498,516
_0409
PP_0410: PP_0410 - DNA-binding response regulator UhpA, at 498,527 to 499,156
_0410
PP_0411: PP_0411 - Polyamine ABC transporter, ATP-binding protein, at 499,452 to 500,576
_0411
PP_0412: PP_0412 - Polyamine ABC transporter, periplasmic polyamine-binding protein, at 500,617 to 501,651
_0412
Position (kb) 499
500
501 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 498.515 kb on - strand at 498.515 kb on - strand at 498.538 kb on - strand at 498.538 kb on - strand at 498.678 kb on + strand, within PP_0410 at 498.678 kb on + strand, within PP_0410 at 498.896 kb on + strand, within PP_0410 at 498.897 kb on - strand, within PP_0410 at 498.898 kb on + strand, within PP_0410 at 498.898 kb on + strand, within PP_0410 at 498.898 kb on + strand, within PP_0410 at 498.899 kb on - strand, within PP_0410 at 498.899 kb on - strand, within PP_0410 at 498.936 kb on + strand, within PP_0410 at 498.937 kb on - strand, within PP_0410 at 498.937 kb on - strand, within PP_0410 at 499.130 kb on + strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.165 kb on + strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.192 kb on + strand at 499.193 kb on - strand at 499.193 kb on - strand at 499.239 kb on + strand at 499.240 kb on - strand at 499.240 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.378 kb on + strand at 499.410 kb on + strand at 499.411 kb on - strand at 499.422 kb on + strand at 499.521 kb on + strand at 499.522 kb on - strand at 499.522 kb on - strand at 499.522 kb on - strand at 499.716 kb on - strand, within PP_0411 at 499.737 kb on + strand, within PP_0411 at 499.737 kb on + strand, within PP_0411 at 499.737 kb on + strand, within PP_0411 at 499.738 kb on - strand, within PP_0411 at 499.951 kb on + strand, within PP_0411 at 499.952 kb on - strand, within PP_0411 at 500.053 kb on - strand, within PP_0411 at 500.053 kb on - strand, within PP_0411 at 500.053 kb on - strand, within PP_0411 at 500.327 kb on - strand, within PP_0411 at 500.406 kb on + strand, within PP_0411 at 500.407 kb on - strand, within PP_0411 at 500.512 kb on + strand at 500.512 kb on + strand at 500.512 kb on + strand at 500.512 kb on + strand at 500.513 kb on - strand at 500.513 kb on - strand at 500.515 kb on + strand at 500.515 kb on + strand at 500.516 kb on - strand at 500.598 kb on + strand at 500.598 kb on + strand at 500.598 kb on + strand at 500.599 kb on - strand at 500.612 kb on + strand at 500.613 kb on - strand at 500.613 kb on - strand at 500.616 kb on - strand at 500.743 kb on + strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.791 kb on + strand, within PP_0412 at 500.792 kb on - strand, within PP_0412 at 500.792 kb on - strand, within PP_0412 at 500.856 kb on - strand, within PP_0412 at 500.944 kb on + strand, within PP_0412 at 500.945 kb on - strand, within PP_0412 at 501.007 kb on + strand, within PP_0412 at 501.008 kb on - strand, within PP_0412 at 501.008 kb on - strand, within PP_0412 at 501.106 kb on + strand, within PP_0412 at 501.107 kb on - strand, within PP_0412 at 501.158 kb on - strand, within PP_0412 at 501.158 kb on - strand, within PP_0412 at 501.298 kb on + strand, within PP_0412 at 501.310 kb on - strand, within PP_0412 at 501.364 kb on + strand, within PP_0412 at 501.365 kb on - strand, within PP_0412 at 501.422 kb on - strand, within PP_0412 at 501.433 kb on + strand, within PP_0412 at 501.463 kb on + strand, within PP_0412 at 501.463 kb on + strand, within PP_0412 at 501.482 kb on - strand, within PP_0412
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 498,515 - +0.8 498,515 - -0.1 498,538 - -2.8 498,538 - +1.5 498,678 + PP_0410 0.24 +0.3 498,678 + PP_0410 0.24 -4.0 498,896 + PP_0410 0.59 -0.4 498,897 - PP_0410 0.59 +1.2 498,898 + PP_0410 0.59 -3.5 498,898 + PP_0410 0.59 +0.7 498,898 + PP_0410 0.59 -0.4 498,899 - PP_0410 0.59 -1.9 498,899 - PP_0410 0.59 +0.8 498,936 + PP_0410 0.65 +1.2 498,937 - PP_0410 0.65 -0.6 498,937 - PP_0410 0.65 +0.1 499,130 + -0.4 499,131 - -1.6 499,131 - +0.7 499,131 - +0.9 499,131 - +0.5 499,131 - +0.6 499,131 - -0.3 499,165 + +1.4 499,166 - -0.4 499,166 - -1.0 499,166 - +1.3 499,166 - +0.7 499,166 - +0.0 499,192 + -2.0 499,193 - -0.6 499,193 - +0.4 499,239 + +1.8 499,240 - -0.3 499,240 - -0.1 499,340 - +1.8 499,340 - +0.5 499,340 - +0.8 499,378 + +1.3 499,410 + -0.2 499,411 - -0.4 499,422 + +0.6 499,521 + -0.3 499,522 - +0.4 499,522 - -0.7 499,522 - +1.1 499,716 - PP_0411 0.23 +1.1 499,737 + PP_0411 0.25 +0.8 499,737 + PP_0411 0.25 +0.0 499,737 + PP_0411 0.25 -1.7 499,738 - PP_0411 0.25 -1.2 499,951 + PP_0411 0.44 +1.1 499,952 - PP_0411 0.44 +0.6 500,053 - PP_0411 0.53 +0.9 500,053 - PP_0411 0.53 +1.0 500,053 - PP_0411 0.53 +1.2 500,327 - PP_0411 0.78 +0.6 500,406 + PP_0411 0.85 +0.8 500,407 - PP_0411 0.85 +0.0 500,512 + -0.7 500,512 + +0.7 500,512 + +0.4 500,512 + +0.0 500,513 - +1.4 500,513 - -0.9 500,515 + -1.5 500,515 + +1.2 500,516 - +0.7 500,598 + -0.0 500,598 + +0.7 500,598 + +0.7 500,599 - -1.8 500,612 + -0.9 500,613 - +0.5 500,613 - +0.0 500,616 - +1.5 500,743 + PP_0412 0.12 -0.3 500,744 - PP_0412 0.12 +0.1 500,744 - PP_0412 0.12 -0.2 500,744 - PP_0412 0.12 -0.8 500,744 - PP_0412 0.12 +0.8 500,791 + PP_0412 0.17 -1.7 500,792 - PP_0412 0.17 +0.0 500,792 - PP_0412 0.17 -2.3 500,856 - PP_0412 0.23 -0.3 500,944 + PP_0412 0.32 -2.9 500,945 - PP_0412 0.32 -0.1 501,007 + PP_0412 0.38 -2.8 501,008 - PP_0412 0.38 +0.3 501,008 - PP_0412 0.38 +1.1 501,106 + PP_0412 0.47 +0.3 501,107 - PP_0412 0.47 +2.4 501,158 - PP_0412 0.52 +0.8 501,158 - PP_0412 0.52 +0.9 501,298 + PP_0412 0.66 -1.0 501,310 - PP_0412 0.67 -0.7 501,364 + PP_0412 0.72 -1.5 501,365 - PP_0412 0.72 +3.7 501,422 - PP_0412 0.78 -1.4 501,433 + PP_0412 0.79 +0.7 501,463 + PP_0412 0.82 -1.5 501,463 + PP_0412 0.82 +0.8 501,482 - PP_0412 0.84 -2.6
Or see this region's nucleotide sequence