Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0303 and PP_0304 are separated by 123 nucleotides PP_0304 and PP_0305 are separated by 132 nucleotides
PP_0303: PP_0303 - dehydrocarnitine cleavage enzyme, at 363,649 to 364,533
_0303
PP_0304: PP_0304 - Carnitine uptake ABC transporter, periplasmic component, at 364,657 to 365,601
_0304
PP_0305: PP_0305 - HTH-type transcriptional regulator CdhR, at 365,734 to 366,693
_0305
Position (kb) 364
365
366 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.426 kb on + strand, within PP_0303 at 364.668 kb on + strand at 364.881 kb on - strand, within PP_0304 at 364.881 kb on - strand, within PP_0304 at 365.159 kb on + strand, within PP_0304 at 365.160 kb on - strand, within PP_0304 at 365.160 kb on - strand, within PP_0304 at 365.225 kb on + strand, within PP_0304 at 365.226 kb on - strand, within PP_0304 at 365.231 kb on + strand, within PP_0304 at 365.317 kb on - strand, within PP_0304 at 365.317 kb on - strand, within PP_0304 at 365.447 kb on - strand, within PP_0304 at 365.447 kb on - strand, within PP_0304 at 365.625 kb on + strand at 365.625 kb on + strand at 365.626 kb on - strand at 365.626 kb on - strand at 365.626 kb on - strand at 365.626 kb on - strand at 365.626 kb on - strand at 365.626 kb on - strand at 365.631 kb on + strand at 365.632 kb on - strand at 365.662 kb on + strand at 365.662 kb on + strand at 365.663 kb on - strand at 365.663 kb on - strand at 365.711 kb on + strand at 365.711 kb on + strand at 365.851 kb on + strand, within PP_0305 at 365.851 kb on + strand, within PP_0305 at 365.868 kb on + strand, within PP_0305 at 365.869 kb on - strand, within PP_0305 at 366.151 kb on + strand, within PP_0305 at 366.521 kb on - strand, within PP_0305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 363,780 - PP_0303 0.15 -0.7 363,780 - PP_0303 0.15 +0.9 363,780 - PP_0303 0.15 -0.8 364,109 + PP_0303 0.52 -0.2 364,109 + PP_0303 0.52 -0.3 364,110 - PP_0303 0.52 -0.1 364,110 - PP_0303 0.52 -1.0 364,426 + PP_0303 0.88 -3.3 364,668 + +0.1 364,881 - PP_0304 0.24 +1.0 364,881 - PP_0304 0.24 -2.0 365,159 + PP_0304 0.53 -3.2 365,160 - PP_0304 0.53 -2.8 365,160 - PP_0304 0.53 +0.1 365,225 + PP_0304 0.60 +1.3 365,226 - PP_0304 0.60 +2.2 365,231 + PP_0304 0.61 +1.3 365,317 - PP_0304 0.70 +0.6 365,317 - PP_0304 0.70 -2.8 365,447 - PP_0304 0.84 +0.2 365,447 - PP_0304 0.84 +0.4 365,625 + -0.2 365,625 + +0.3 365,626 - +0.6 365,626 - -0.7 365,626 - -0.2 365,626 - +0.5 365,626 - -0.8 365,626 - -1.3 365,631 + -0.1 365,632 - -2.8 365,662 + +0.8 365,662 + -1.5 365,663 - -1.0 365,663 - +0.7 365,711 + -4.6 365,711 + -0.4 365,851 + PP_0305 0.12 -2.7 365,851 + PP_0305 0.12 +0.5 365,868 + PP_0305 0.14 -3.5 365,869 - PP_0305 0.14 +0.0 366,151 + PP_0305 0.43 +0.9 366,521 - PP_0305 0.82 +0.1
Or see this region's nucleotide sequence