Strain Fitness in Pseudomonas putida KT2440 around PP_0191

Experiment: R2A with Fusaric 240 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0189 and PP_0190 are separated by 222 nucleotidesPP_0190 and PP_0191 are separated by 231 nucleotidesPP_0191 and PP_0192 are separated by 8 nucleotidesPP_0192 and PP_0194 are separated by 154 nucleotides PP_0189: PP_0189 - putative heme biosynthesis protein, at 240,830 to 242,068 _0189 PP_0190: PP_0190 - putative disulfide bond formation protein B, at 242,291 to 242,797 _0190 PP_0191: PP_0191 - Transcriptional regulatory protein AlgQ, at 243,029 to 243,499 _0191 PP_0192: PP_0192 - putative FKBP-type 25 kDa peptidyl-prolyl cis-trans isomerase, at 243,508 to 244,170 _0192 PP_0194: PP_0194 - Transcriptional regulatory protein AlgP, at 244,325 to 245,314 _0194 Position (kb) 243 244Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 242.112 kb on + strandat 242.117 kb on + strandat 242.166 kb on + strandat 242.173 kb on + strandat 242.174 kb on - strandat 242.349 kb on + strand, within PP_0190at 242.349 kb on + strand, within PP_0190at 242.350 kb on - strand, within PP_0190at 242.350 kb on - strand, within PP_0190at 242.496 kb on - strandat 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.768 kb on + strandat 242.840 kb on - strandat 242.840 kb on - strandat 242.840 kb on - strandat 242.847 kb on + strandat 242.850 kb on + strandat 242.860 kb on + strandat 242.880 kb on + strandat 242.880 kb on + strandat 242.907 kb on + strandat 242.908 kb on - strandat 242.924 kb on + strandat 243.006 kb on + strandat 243.037 kb on + strandat 243.038 kb on - strandat 243.203 kb on + strand, within PP_0191at 243.203 kb on + strand, within PP_0191at 243.319 kb on + strand, within PP_0191at 243.319 kb on + strand, within PP_0191at 243.554 kb on + strandat 243.554 kb on + strandat 243.554 kb on + strandat 243.555 kb on - strandat 243.590 kb on + strand, within PP_0192at 243.590 kb on + strand, within PP_0192at 243.610 kb on + strand, within PP_0192at 243.611 kb on - strand, within PP_0192at 243.743 kb on + strand, within PP_0192at 243.744 kb on - strand, within PP_0192at 243.744 kb on - strand, within PP_0192at 243.744 kb on - strand, within PP_0192at 243.807 kb on - strand, within PP_0192at 243.824 kb on - strand, within PP_0192at 243.824 kb on - strand, within PP_0192at 243.839 kb on + strand, within PP_0192at 243.839 kb on + strand, within PP_0192at 243.856 kb on - strand, within PP_0192at 243.978 kb on - strand, within PP_0192at 244.067 kb on + strand, within PP_0192at 244.068 kb on - strand, within PP_0192at 244.136 kb on + strandat 244.137 kb on - strandat 244.162 kb on + strandat 244.187 kb on + strandat 244.187 kb on + strandat 244.188 kb on - strandat 244.188 kb on - strandat 244.188 kb on - strandat 244.210 kb on + strandat 244.242 kb on - strandat 244.249 kb on - strandat 244.287 kb on + strandat 244.287 kb on + strandat 244.287 kb on + strandat 244.287 kb on + strandat 244.288 kb on - strandat 244.288 kb on - strandat 244.288 kb on - strandat 244.288 kb on - strandat 244.288 kb on - strandat 244.290 kb on + strandat 244.290 kb on + strandat 244.290 kb on + strandat 244.290 kb on + strandat 244.291 kb on - strandat 244.291 kb on - strandat 244.319 kb on + strandat 244.347 kb on + strandat 244.351 kb on + strandat 244.352 kb on - strandat 244.359 kb on + strandat 244.360 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL
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242,112 + +0.8
242,117 + -0.3
242,166 + +0.3
242,173 + +0.7
242,174 - -0.6
242,349 + PP_0190 0.11 -0.5
242,349 + PP_0190 0.11 +0.1
242,350 - PP_0190 0.12 -0.3
242,350 - PP_0190 0.12 +1.1
242,496 - -0.3
242,587 - PP_0190 0.58 -0.0
242,587 - PP_0190 0.58 +0.2
242,587 - PP_0190 0.58 -4.0
242,587 - PP_0190 0.58 +0.1
242,587 - PP_0190 0.58 -0.1
242,768 + +0.9
242,840 - +0.5
242,840 - +1.0
242,840 - -0.3
242,847 + -0.4
242,850 + +0.2
242,860 + -1.0
242,880 + -1.6
242,880 + +0.5
242,907 + -0.2
242,908 - +1.2
242,924 + +0.1
243,006 + +0.8
243,037 + +0.1
243,038 - +0.8
243,203 + PP_0191 0.37 +0.3
243,203 + PP_0191 0.37 +0.5
243,319 + PP_0191 0.62 +1.7
243,319 + PP_0191 0.62 +0.5
243,554 + +0.7
243,554 + +0.2
243,554 + -0.5
243,555 - +1.2
243,590 + PP_0192 0.12 +0.7
243,590 + PP_0192 0.12 +0.5
243,610 + PP_0192 0.15 -0.5
243,611 - PP_0192 0.16 +0.4
243,743 + PP_0192 0.35 -0.1
243,744 - PP_0192 0.36 +0.8
243,744 - PP_0192 0.36 +0.6
243,744 - PP_0192 0.36 +0.7
243,807 - PP_0192 0.45 -0.3
243,824 - PP_0192 0.48 -0.2
243,824 - PP_0192 0.48 -1.2
243,839 + PP_0192 0.50 -0.9
243,839 + PP_0192 0.50 +0.6
243,856 - PP_0192 0.52 -0.9
243,978 - PP_0192 0.71 +0.4
244,067 + PP_0192 0.84 -1.4
244,068 - PP_0192 0.84 +0.3
244,136 + +0.5
244,137 - +1.0
244,162 + -3.9
244,187 + -0.5
244,187 + +0.1
244,188 - -1.8
244,188 - +1.3
244,188 - +2.1
244,210 + +0.8
244,242 - -1.8
244,249 - +1.4
244,287 + +1.6
244,287 + -0.7
244,287 + -0.6
244,287 + -0.1
244,288 - -3.8
244,288 - +2.0
244,288 - +0.6
244,288 - -0.6
244,288 - -2.2
244,290 + -0.5
244,290 + -2.3
244,290 + -0.4
244,290 + +1.7
244,291 - -4.0
244,291 - -0.8
244,319 + -0.3
244,347 + -0.0
244,351 + -0.3
244,352 - -1.4
244,359 + +1.0
244,360 - +0.8

Or see this region's nucleotide sequence