Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0022 and PP_0023 are separated by 445 nucleotides PP_0023 and PP_0024 are separated by 122 nucleotides PP_0024 and PP_0025 are separated by 442 nucleotides PP_0025 and PP_0026 are separated by 288 nucleotides
PP_0022: PP_0022 - hypothetical protein, at 26,989 to 27,153
_0022
PP_0023: PP_0023 - conserved hypothetical protein, at 27,599 to 27,727
_0023
PP_0024: PP_0024 - putative membrane-associated metal-dependent hydrolase, at 27,850 to 29,547
_0024
PP_0025: PP_0025 - hypothetical protein, at 29,990 to 30,142
_0025
PP_0026: PP_0026 - putative cobalt/cadmium/zinc exporter, at 30,431 to 31,336
_0026
Position (kb) 27
28
29
30 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 26.851 kb on + strand at 26.851 kb on + strand at 26.851 kb on + strand at 26.852 kb on - strand at 26.871 kb on + strand at 26.878 kb on + strand at 26.879 kb on - strand at 26.879 kb on - strand at 26.882 kb on + strand at 26.882 kb on + strand at 26.883 kb on - strand at 26.955 kb on + strand at 26.955 kb on + strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.991 kb on - strand at 26.992 kb on + strand at 26.993 kb on - strand at 26.993 kb on - strand at 26.993 kb on - strand at 27.091 kb on - strand, within PP_0022 at 27.140 kb on + strand at 27.140 kb on + strand at 27.141 kb on - strand at 27.173 kb on - strand at 27.211 kb on - strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.321 kb on + strand at 27.322 kb on - strand at 27.322 kb on - strand at 27.322 kb on - strand at 27.432 kb on + strand at 27.433 kb on - strand at 27.462 kb on - strand at 27.476 kb on + strand at 27.476 kb on + strand at 27.600 kb on + strand at 27.601 kb on - strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.767 kb on - strand at 27.767 kb on - strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.771 kb on - strand at 27.771 kb on - strand at 27.774 kb on + strand at 27.775 kb on - strand at 27.775 kb on - strand at 27.863 kb on + strand at 27.863 kb on + strand at 27.864 kb on - strand at 27.864 kb on - strand at 27.864 kb on - strand at 27.885 kb on + strand at 27.885 kb on + strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.917 kb on + strand at 28.054 kb on + strand, within PP_0024 at 28.055 kb on - strand, within PP_0024 at 28.055 kb on - strand, within PP_0024 at 28.094 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.112 kb on + strand, within PP_0024 at 28.113 kb on - strand, within PP_0024 at 28.113 kb on - strand, within PP_0024 at 28.154 kb on + strand, within PP_0024 at 28.155 kb on - strand, within PP_0024 at 28.155 kb on - strand, within PP_0024 at 28.196 kb on + strand, within PP_0024 at 28.217 kb on + strand, within PP_0024 at 28.217 kb on + strand, within PP_0024 at 28.217 kb on + strand, within PP_0024 at 28.217 kb on + strand, within PP_0024 at 28.217 kb on + strand, within PP_0024 at 28.218 kb on - strand, within PP_0024 at 28.218 kb on - strand, within PP_0024 at 28.226 kb on + strand, within PP_0024 at 28.244 kb on + strand, within PP_0024 at 28.244 kb on + strand, within PP_0024 at 28.269 kb on + strand, within PP_0024 at 28.269 kb on + strand, within PP_0024 at 28.269 kb on + strand, within PP_0024 at 28.269 kb on + strand, within PP_0024 at 28.270 kb on - strand, within PP_0024 at 28.337 kb on + strand, within PP_0024 at 28.337 kb on + strand, within PP_0024 at 28.337 kb on + strand, within PP_0024 at 28.337 kb on + strand, within PP_0024 at 28.337 kb on + strand at 28.337 kb on + strand, within PP_0024 at 28.338 kb on - strand, within PP_0024 at 28.338 kb on - strand, within PP_0024 at 28.338 kb on - strand, within PP_0024 at 28.338 kb on - strand, within PP_0024 at 28.341 kb on - strand, within PP_0024 at 28.341 kb on - strand, within PP_0024 at 28.341 kb on - strand, within PP_0024 at 28.341 kb on - strand, within PP_0024 at 28.341 kb on - strand, within PP_0024 at 28.364 kb on + strand, within PP_0024 at 28.364 kb on + strand, within PP_0024 at 28.364 kb on + strand, within PP_0024 at 28.365 kb on - strand, within PP_0024 at 28.365 kb on - strand, within PP_0024 at 28.440 kb on + strand, within PP_0024 at 28.440 kb on + strand, within PP_0024 at 28.440 kb on + strand, within PP_0024 at 28.440 kb on + strand, within PP_0024 at 28.441 kb on - strand, within PP_0024 at 28.441 kb on - strand, within PP_0024 at 28.441 kb on - strand, within PP_0024 at 28.441 kb on - strand, within PP_0024 at 28.441 kb on - strand, within PP_0024 at 28.613 kb on + strand, within PP_0024 at 28.614 kb on - strand, within PP_0024 at 28.695 kb on + strand, within PP_0024 at 28.695 kb on + strand, within PP_0024 at 28.695 kb on + strand at 28.695 kb on + strand, within PP_0024 at 28.696 kb on - strand, within PP_0024 at 28.696 kb on - strand, within PP_0024 at 28.696 kb on - strand, within PP_0024 at 28.714 kb on + strand, within PP_0024 at 28.715 kb on - strand, within PP_0024 at 28.790 kb on - strand, within PP_0024 at 28.807 kb on - strand, within PP_0024 at 28.831 kb on + strand, within PP_0024 at 28.832 kb on - strand, within PP_0024 at 28.832 kb on - strand, within PP_0024 at 28.832 kb on - strand, within PP_0024 at 28.832 kb on - strand, within PP_0024 at 28.842 kb on + strand, within PP_0024 at 28.842 kb on + strand, within PP_0024 at 28.842 kb on + strand, within PP_0024 at 28.863 kb on + strand, within PP_0024 at 28.863 kb on + strand, within PP_0024 at 28.864 kb on - strand, within PP_0024 at 28.864 kb on - strand, within PP_0024 at 28.889 kb on + strand, within PP_0024 at 28.917 kb on + strand, within PP_0024 at 28.917 kb on + strand, within PP_0024 at 28.918 kb on - strand, within PP_0024 at 28.918 kb on - strand, within PP_0024 at 28.967 kb on + strand, within PP_0024 at 28.967 kb on + strand, within PP_0024 at 28.967 kb on + strand, within PP_0024 at 28.968 kb on - strand, within PP_0024 at 28.968 kb on - strand, within PP_0024 at 28.968 kb on - strand, within PP_0024 at 28.968 kb on - strand, within PP_0024 at 28.968 kb on - strand, within PP_0024 at 28.968 kb on - strand, within PP_0024 at 28.976 kb on + strand, within PP_0024 at 28.976 kb on + strand, within PP_0024 at 28.999 kb on + strand, within PP_0024 at 28.999 kb on + strand, within PP_0024 at 29.000 kb on - strand, within PP_0024 at 29.000 kb on - strand, within PP_0024 at 29.090 kb on + strand, within PP_0024 at 29.090 kb on + strand, within PP_0024 at 29.091 kb on - strand, within PP_0024 at 29.195 kb on + strand, within PP_0024 at 29.196 kb on - strand, within PP_0024 at 29.229 kb on - strand, within PP_0024 at 29.229 kb on - strand, within PP_0024 at 29.255 kb on - strand, within PP_0024 at 29.266 kb on + strand, within PP_0024 at 29.267 kb on - strand, within PP_0024 at 29.311 kb on - strand, within PP_0024 at 29.327 kb on - strand, within PP_0024 at 29.327 kb on - strand, within PP_0024 at 29.327 kb on - strand, within PP_0024 at 29.327 kb on - strand, within PP_0024 at 29.329 kb on + strand, within PP_0024 at 29.329 kb on + strand, within PP_0024 at 29.330 kb on - strand, within PP_0024 at 29.381 kb on - strand at 29.453 kb on - strand at 29.569 kb on + strand at 29.582 kb on - strand at 29.586 kb on - strand at 29.586 kb on - strand at 29.586 kb on - strand at 29.593 kb on + strand at 29.603 kb on + strand at 29.604 kb on - strand at 29.689 kb on + strand at 29.689 kb on + strand at 29.692 kb on + strand at 29.692 kb on + strand at 29.693 kb on - strand at 29.711 kb on + strand at 29.854 kb on + strand at 29.855 kb on - strand at 29.857 kb on + strand at 29.891 kb on - strand at 30.009 kb on - strand, within PP_0025 at 30.131 kb on + strand at 30.132 kb on - strand at 30.170 kb on - strand at 30.179 kb on + strand at 30.179 kb on + strand at 30.179 kb on + strand at 30.179 kb on + strand at 30.179 kb on + strand at 30.180 kb on - strand at 30.180 kb on - strand at 30.374 kb on + strand at 30.374 kb on + strand at 30.374 kb on + strand at 30.375 kb on - strand at 30.375 kb on - strand at 30.375 kb on - strand at 30.381 kb on - strand at 30.381 kb on - strand at 30.381 kb on - strand at 30.381 kb on - strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.385 kb on - strand at 30.385 kb on - strand at 30.428 kb on + strand at 30.428 kb on + strand at 30.428 kb on + strand at 30.428 kb on + strand at 30.428 kb on + strand at 30.428 kb on + strand at 30.429 kb on - strand at 30.429 kb on - strand at 30.429 kb on - strand at 30.429 kb on - strand at 30.429 kb on - strand at 30.465 kb on + strand at 30.490 kb on - strand at 30.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 26,851 + -0.3 26,851 + +0.3 26,851 + +1.2 26,852 - +0.1 26,871 + -2.0 26,878 + -0.6 26,879 - +0.1 26,879 - +0.8 26,882 + +0.3 26,882 + -0.5 26,883 - -1.9 26,955 + +1.0 26,955 + +0.8 26,956 - +0.9 26,956 - +1.1 26,956 - -0.8 26,956 - +1.6 26,956 - -2.6 26,956 - -0.7 26,956 - -1.3 26,990 + +0.2 26,990 + -1.4 26,990 + -1.2 26,990 + +0.3 26,991 - +1.1 26,992 + +1.0 26,993 - -0.1 26,993 - -2.9 26,993 - -1.6 27,091 - PP_0022 0.62 +0.5 27,140 + -1.4 27,140 + +0.1 27,141 - -0.7 27,173 - +0.7 27,211 - -1.8 27,291 + +0.3 27,291 + +0.7 27,291 + +0.4 27,291 + +0.1 27,291 + +1.0 27,291 + -0.3 27,291 + -0.3 27,291 + +0.2 27,291 + +1.3 27,292 - -4.0 27,292 - +0.2 27,292 - -2.5 27,292 - +0.6 27,292 - +1.3 27,292 - +0.3 27,292 - +1.2 27,292 - +0.3 27,292 - +1.0 27,292 - -0.3 27,292 - -0.6 27,292 - +0.2 27,292 - +1.7 27,292 - +1.2 27,292 - +0.6 27,321 + +0.3 27,322 - -0.1 27,322 - +0.0 27,322 - -1.2 27,432 + +0.7 27,433 - -3.7 27,462 - -1.1 27,476 + +0.3 27,476 + +0.9 27,600 + -0.4 27,601 - +0.5 27,766 + +0.0 27,766 + -1.6 27,766 + +0.4 27,766 + -1.7 27,767 - +0.3 27,767 - -0.7 27,770 + +1.4 27,770 + +0.7 27,770 + -0.2 27,770 + +1.2 27,770 + +0.3 27,771 - +0.9 27,771 - +0.6 27,774 + +0.4 27,775 - -1.0 27,775 - -0.5 27,863 + -3.3 27,863 + -1.0 27,864 - +0.8 27,864 - +1.2 27,864 - -0.6 27,885 + +0.0 27,885 + -0.1 27,886 - +2.2 27,886 - -1.3 27,886 - +0.7 27,886 - -0.6 27,886 - +1.3 27,917 + +0.2 28,054 + PP_0024 0.12 -0.3 28,055 - PP_0024 0.12 +1.2 28,055 - PP_0024 0.12 +1.6 28,094 + PP_0024 0.14 -0.3 28,108 + PP_0024 0.15 +1.9 28,108 + PP_0024 0.15 +2.6 28,108 + PP_0024 0.15 +0.2 28,108 + PP_0024 0.15 +1.0 28,109 - PP_0024 0.15 -0.5 28,109 - PP_0024 0.15 -1.2 28,109 - PP_0024 0.15 +0.7 28,112 + PP_0024 0.15 +0.2 28,113 - PP_0024 0.15 +0.4 28,113 - PP_0024 0.15 +1.1 28,154 + PP_0024 0.18 +0.9 28,155 - PP_0024 0.18 +0.6 28,155 - PP_0024 0.18 +0.3 28,196 + PP_0024 0.20 +0.1 28,217 + PP_0024 0.22 -0.8 28,217 + PP_0024 0.22 -0.8 28,217 + PP_0024 0.22 +0.5 28,217 + PP_0024 0.22 -1.0 28,217 + PP_0024 0.22 -0.6 28,218 - PP_0024 0.22 -0.5 28,218 - PP_0024 0.22 -0.7 28,226 + PP_0024 0.22 +0.1 28,244 + PP_0024 0.23 +0.2 28,244 + PP_0024 0.23 +1.4 28,269 + PP_0024 0.25 +0.1 28,269 + PP_0024 0.25 -0.8 28,269 + PP_0024 0.25 -0.9 28,269 + PP_0024 0.25 -2.0 28,270 - PP_0024 0.25 +1.0 28,337 + PP_0024 0.29 -0.3 28,337 + PP_0024 0.29 -1.4 28,337 + PP_0024 0.29 -0.3 28,337 + PP_0024 0.29 +0.6 28,337 + -0.4 28,337 + PP_0024 0.29 +1.3 28,338 - PP_0024 0.29 +0.4 28,338 - PP_0024 0.29 -0.3 28,338 - PP_0024 0.29 +0.1 28,338 - PP_0024 0.29 -0.0 28,341 - PP_0024 0.29 +0.1 28,341 - PP_0024 0.29 +0.9 28,341 - PP_0024 0.29 +0.7 28,341 - PP_0024 0.29 +1.8 28,341 - PP_0024 0.29 +0.7 28,364 + PP_0024 0.30 +0.4 28,364 + PP_0024 0.30 +0.4 28,364 + PP_0024 0.30 +0.3 28,365 - PP_0024 0.30 -0.8 28,365 - PP_0024 0.30 +1.2 28,440 + PP_0024 0.35 -0.8 28,440 + PP_0024 0.35 -0.4 28,440 + PP_0024 0.35 +0.2 28,440 + PP_0024 0.35 +0.6 28,441 - PP_0024 0.35 +0.8 28,441 - PP_0024 0.35 +0.5 28,441 - PP_0024 0.35 +0.2 28,441 - PP_0024 0.35 +0.9 28,441 - PP_0024 0.35 +0.3 28,613 + PP_0024 0.45 -0.1 28,614 - PP_0024 0.45 -0.2 28,695 + PP_0024 0.50 -1.4 28,695 + PP_0024 0.50 -0.9 28,695 + +1.2 28,695 + PP_0024 0.50 +0.7 28,696 - PP_0024 0.50 -3.3 28,696 - PP_0024 0.50 +0.0 28,696 - PP_0024 0.50 -0.3 28,714 + PP_0024 0.51 +0.6 28,715 - PP_0024 0.51 +1.0 28,790 - PP_0024 0.55 +1.2 28,807 - PP_0024 0.56 -0.1 28,831 + PP_0024 0.58 -0.7 28,832 - PP_0024 0.58 +1.2 28,832 - PP_0024 0.58 -0.1 28,832 - PP_0024 0.58 +1.1 28,832 - PP_0024 0.58 +1.9 28,842 + PP_0024 0.58 +2.2 28,842 + PP_0024 0.58 +2.5 28,842 + PP_0024 0.58 +0.8 28,863 + PP_0024 0.60 +1.7 28,863 + PP_0024 0.60 +0.2 28,864 - PP_0024 0.60 -1.2 28,864 - PP_0024 0.60 +1.0 28,889 + PP_0024 0.61 -1.5 28,917 + PP_0024 0.63 -1.1 28,917 + PP_0024 0.63 -0.3 28,918 - PP_0024 0.63 -4.1 28,918 - PP_0024 0.63 +0.9 28,967 + PP_0024 0.66 +0.3 28,967 + PP_0024 0.66 +0.7 28,967 + PP_0024 0.66 -0.8 28,968 - PP_0024 0.66 +1.0 28,968 - PP_0024 0.66 -1.7 28,968 - PP_0024 0.66 +0.4 28,968 - PP_0024 0.66 +1.0 28,968 - PP_0024 0.66 +0.1 28,968 - PP_0024 0.66 +1.4 28,976 + PP_0024 0.66 +1.3 28,976 + PP_0024 0.66 +0.6 28,999 + PP_0024 0.68 +0.6 28,999 + PP_0024 0.68 +0.9 29,000 - PP_0024 0.68 -4.4 29,000 - PP_0024 0.68 +1.1 29,090 + PP_0024 0.73 +3.2 29,090 + PP_0024 0.73 -2.9 29,091 - PP_0024 0.73 -0.9 29,195 + PP_0024 0.79 +0.3 29,196 - PP_0024 0.79 +1.4 29,229 - PP_0024 0.81 +0.1 29,229 - PP_0024 0.81 +1.8 29,255 - PP_0024 0.83 +1.4 29,266 + PP_0024 0.83 +1.8 29,267 - PP_0024 0.83 -3.1 29,311 - PP_0024 0.86 -0.2 29,327 - PP_0024 0.87 +1.2 29,327 - PP_0024 0.87 -2.2 29,327 - PP_0024 0.87 +0.2 29,327 - PP_0024 0.87 +1.6 29,329 + PP_0024 0.87 -1.1 29,329 + PP_0024 0.87 +0.1 29,330 - PP_0024 0.87 -0.4 29,381 - +0.2 29,453 - -2.7 29,569 + -3.0 29,582 - +0.9 29,586 - +0.7 29,586 - +0.5 29,586 - -0.9 29,593 + -0.8 29,603 + -2.3 29,604 - +0.8 29,689 + +0.7 29,689 + +1.7 29,692 + +0.5 29,692 + +0.3 29,693 - -2.1 29,711 + +0.7 29,854 + -1.5 29,855 - +1.1 29,857 + +1.2 29,891 - +0.1 30,009 - PP_0025 0.12 +0.9 30,131 + +0.9 30,132 - -0.2 30,170 - +0.1 30,179 + -1.8 30,179 + +1.0 30,179 + +0.3 30,179 + -0.0 30,179 + -0.1 30,180 - -2.1 30,180 - -0.0 30,374 + +1.1 30,374 + -2.3 30,374 + +1.2 30,375 - -0.4 30,375 - -1.9 30,375 - +1.7 30,381 - -1.9 30,381 - -0.1 30,381 - -3.0 30,381 - -2.4 30,384 + +0.9 30,384 + -0.0 30,384 + +0.2 30,384 + +0.6 30,385 - +0.2 30,385 - +1.0 30,428 + -0.6 30,428 + -1.0 30,428 + -0.2 30,428 + +0.4 30,428 + +0.2 30,428 + -0.0 30,429 - +0.9 30,429 - -3.1 30,429 - +0.2 30,429 - +0.8 30,429 - -2.4 30,465 + -0.3 30,490 - -1.0 30,490 - -0.3
Or see this region's nucleotide sequence