Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1407 and PP_t64 are separated by 210 nucleotides PP_t64 and PP_1408 are separated by 111 nucleotides
PP_1407: PP_1407 - Sulfate transporter, at 1,604,544 to 1,606,250
_1407
PP_t64: PP_t64 - tRNA-Gly, at 1,606,461 to 1,606,534
_t64
PP_1408: PP_1408 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase, at 1,606,646 to 1,607,533
_1408
Position (kb)
1606
1607 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1605.552 kb on + strand, within PP_1407 at 1605.624 kb on + strand, within PP_1407 at 1606.047 kb on - strand, within PP_1407 at 1606.060 kb on + strand, within PP_1407 at 1606.075 kb on - strand, within PP_1407 at 1606.087 kb on + strand at 1606.087 kb on + strand at 1606.088 kb on - strand at 1606.097 kb on - strand at 1606.097 kb on - strand at 1606.097 kb on - strand at 1606.126 kb on + strand at 1606.267 kb on + strand at 1606.291 kb on - strand at 1606.291 kb on - strand at 1606.356 kb on + strand at 1606.357 kb on - strand at 1606.492 kb on + strand, within PP_t64 at 1606.493 kb on - strand, within PP_t64 at 1606.526 kb on + strand, within PP_t64 at 1606.526 kb on + strand at 1606.526 kb on + strand, within PP_t64 at 1606.527 kb on - strand at 1606.527 kb on - strand at 1606.528 kb on + strand at 1606.529 kb on - strand at 1606.585 kb on + strand at 1606.588 kb on - strand at 1606.628 kb on + strand at 1606.629 kb on - strand at 1606.687 kb on - strand at 1606.861 kb on - strand, within PP_1408 at 1606.903 kb on + strand, within PP_1408 at 1606.977 kb on + strand, within PP_1408 at 1607.010 kb on + strand, within PP_1408 at 1607.011 kb on - strand at 1607.011 kb on - strand, within PP_1408 at 1607.011 kb on - strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.122 kb on - strand, within PP_1408 at 1607.181 kb on + strand, within PP_1408 at 1607.282 kb on - strand, within PP_1408 at 1607.282 kb on - strand, within PP_1408 at 1607.346 kb on + strand, within PP_1408 at 1607.346 kb on + strand, within PP_1408 at 1607.346 kb on + strand at 1607.347 kb on - strand, within PP_1408 at 1607.389 kb on + strand, within PP_1408 at 1607.393 kb on + strand, within PP_1408 at 1607.393 kb on + strand, within PP_1408 at 1607.394 kb on - strand, within PP_1408 at 1607.472 kb on + strand at 1607.485 kb on - strand at 1607.514 kb on - strand at 1607.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 1,605,552 + PP_1407 0.59 -0.1 1,605,624 + PP_1407 0.63 -0.1 1,606,047 - PP_1407 0.88 -2.8 1,606,060 + PP_1407 0.89 -0.4 1,606,075 - PP_1407 0.90 -0.6 1,606,087 + -0.5 1,606,087 + +0.8 1,606,088 - +0.2 1,606,097 - +0.1 1,606,097 - -0.1 1,606,097 - +0.4 1,606,126 + -0.2 1,606,267 + -0.2 1,606,291 - -0.7 1,606,291 - -0.1 1,606,356 + -2.3 1,606,357 - +0.3 1,606,492 + PP_t64 0.42 -0.2 1,606,493 - PP_t64 0.43 -1.2 1,606,526 + PP_t64 0.88 -0.3 1,606,526 + +0.3 1,606,526 + PP_t64 0.88 -1.8 1,606,527 - +0.6 1,606,527 - -0.4 1,606,528 + +0.0 1,606,529 - -1.4 1,606,585 + +0.6 1,606,588 - -0.6 1,606,628 + +0.6 1,606,629 - +0.3 1,606,687 - +0.5 1,606,861 - PP_1408 0.24 +1.2 1,606,903 + PP_1408 0.29 -0.4 1,606,977 + PP_1408 0.37 +0.4 1,607,010 + PP_1408 0.41 -0.3 1,607,011 - +0.2 1,607,011 - PP_1408 0.41 +0.1 1,607,011 - PP_1408 0.41 +0.3 1,607,100 + PP_1408 0.51 +0.3 1,607,100 + PP_1408 0.51 -0.3 1,607,100 + PP_1408 0.51 +0.1 1,607,100 + PP_1408 0.51 +0.0 1,607,122 - PP_1408 0.54 +1.5 1,607,181 + PP_1408 0.60 +0.3 1,607,282 - PP_1408 0.72 +0.1 1,607,282 - PP_1408 0.72 -0.3 1,607,346 + PP_1408 0.79 +1.0 1,607,346 + PP_1408 0.79 -0.1 1,607,346 + -0.6 1,607,347 - PP_1408 0.79 +0.1 1,607,389 + PP_1408 0.84 +0.6 1,607,393 + PP_1408 0.84 -0.9 1,607,393 + PP_1408 0.84 -0.1 1,607,394 - PP_1408 0.84 -0.5 1,607,472 + +1.1 1,607,485 - -0.3 1,607,514 - +0.4 1,607,514 - -0.2
Or see this region's nucleotide sequence