Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4320 and PP_5748 overlap by 8 nucleotides PP_5748 and PP_4321 overlap by 4 nucleotides PP_4321 and PP_4322 overlap by 4 nucleotides
PP_4320: PP_4320 - cytochrome c-type biogenesis protein, at 4,910,515 to 4,911,711
_4320
PP_5748: PP_5748 - cytochrome c heme lyase subunit, at 4,911,704 to 4,912,180
_5748
PP_4321: PP_4321 - holocytochrome c synthetase subunit, at 4,912,177 to 4,912,713
_4321
PP_4322: PP_4322 - holocytochrome c synthetase, at 4,912,710 to 4,914,698
_4322
Position (kb)
4911
4912
4913 Strain fitness (log2 ratio)
-2
-1
0
1 at 4910.781 kb on + strand, within PP_4320 at 4910.782 kb on - strand, within PP_4320 at 4910.919 kb on + strand, within PP_4320 at 4910.984 kb on + strand, within PP_4320 at 4911.003 kb on - strand, within PP_4320 at 4911.198 kb on + strand, within PP_4320 at 4911.233 kb on + strand, within PP_4320 at 4911.234 kb on - strand, within PP_4320 at 4911.251 kb on + strand, within PP_4320 at 4911.251 kb on + strand, within PP_4320 at 4911.252 kb on - strand, within PP_4320 at 4911.252 kb on - strand, within PP_4320 at 4911.303 kb on + strand, within PP_4320 at 4911.384 kb on - strand at 4912.108 kb on - strand, within PP_5748 at 4912.108 kb on - strand, within PP_5748 at 4912.314 kb on - strand at 4912.854 kb on - strand at 4912.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 4,910,781 + PP_4320 0.22 -0.0 4,910,782 - PP_4320 0.22 -0.4 4,910,919 + PP_4320 0.34 -0.4 4,910,984 + PP_4320 0.39 +1.0 4,911,003 - PP_4320 0.41 +0.1 4,911,198 + PP_4320 0.57 -0.0 4,911,233 + PP_4320 0.60 +0.6 4,911,234 - PP_4320 0.60 -2.4 4,911,251 + PP_4320 0.61 +0.3 4,911,251 + PP_4320 0.61 +0.2 4,911,252 - PP_4320 0.62 +1.0 4,911,252 - PP_4320 0.62 +1.3 4,911,303 + PP_4320 0.66 -1.7 4,911,384 - +1.3 4,912,108 - PP_5748 0.85 -1.0 4,912,108 - PP_5748 0.85 -0.5 4,912,314 - -0.3 4,912,854 - -0.6 4,912,858 - -1.5
Or see this region's nucleotide sequence