Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5052 and PP_5053 are separated by 155 nucleotides PP_5053 and PP_5054 are separated by 39 nucleotides PP_5054 and PP_5055 are separated by 1 nucleotides PP_5055 and PP_5056 are separated by 207 nucleotides
PP_5052: PP_5052 - tRNA(Leu) mC34,mU34 2'-O-ribose methyltransferase, AdoMet-dependent, at 5,758,918 to 5,759,391
_5052
PP_5053: PP_5053 - Protein-export protein SecB, at 5,759,547 to 5,760,032
_5053
PP_5054: PP_5054 - glutaredoxin 3, at 5,760,072 to 5,760,326
_5054
PP_5055: PP_5055 - Sulfurtransferase, at 5,760,328 to 5,760,741
_5055
PP_5056: PP_5056 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 5,760,949 to 5,762,484
_5056
Position (kb) 5760
5761 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5759.335 kb on - strand, within PP_5052 at 5759.353 kb on + strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.433 kb on + strand at 5759.477 kb on + strand at 5759.545 kb on - strand at 5759.549 kb on - strand at 5759.549 kb on - strand at 5759.558 kb on + strand at 5759.559 kb on - strand at 5759.559 kb on - strand at 5759.582 kb on + strand at 5759.602 kb on + strand, within PP_5053 at 5759.603 kb on - strand, within PP_5053 at 5759.766 kb on - strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5760.043 kb on + strand at 5760.048 kb on + strand at 5760.048 kb on + strand at 5760.048 kb on + strand at 5760.118 kb on + strand, within PP_5054 at 5760.119 kb on - strand, within PP_5054 at 5760.119 kb on - strand, within PP_5054 at 5760.119 kb on - strand, within PP_5054 at 5760.269 kb on - strand, within PP_5054 at 5760.296 kb on - strand, within PP_5054 at 5760.307 kb on + strand at 5760.326 kb on + strand at 5760.326 kb on + strand at 5760.326 kb on + strand at 5760.327 kb on - strand at 5760.327 kb on - strand at 5760.327 kb on - strand at 5760.327 kb on - strand at 5760.395 kb on + strand, within PP_5055 at 5760.395 kb on + strand, within PP_5055 at 5760.474 kb on + strand, within PP_5055 at 5760.540 kb on + strand, within PP_5055 at 5760.541 kb on - strand, within PP_5055 at 5760.541 kb on - strand, within PP_5055 at 5760.554 kb on + strand, within PP_5055 at 5760.555 kb on - strand, within PP_5055 at 5760.704 kb on + strand at 5760.704 kb on + strand at 5760.704 kb on + strand at 5760.705 kb on - strand at 5760.705 kb on - strand at 5760.705 kb on - strand at 5760.705 kb on - strand at 5760.779 kb on + strand at 5760.779 kb on + strand at 5760.779 kb on + strand at 5760.779 kb on + strand at 5760.779 kb on + strand at 5760.780 kb on - strand at 5760.780 kb on - strand at 5760.780 kb on - strand at 5760.780 kb on - strand at 5760.780 kb on - strand at 5760.876 kb on + strand at 5760.877 kb on - strand at 5760.877 kb on - strand at 5760.894 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 5,759,335 - PP_5052 0.88 +0.2 5,759,353 + -0.1 5,759,354 - -0.1 5,759,354 - +0.0 5,759,354 - +0.5 5,759,354 - +0.2 5,759,354 - -0.0 5,759,433 + -1.2 5,759,477 + +0.3 5,759,545 - -1.2 5,759,549 - -1.3 5,759,549 - +1.0 5,759,558 + +0.3 5,759,559 - +1.9 5,759,559 - +0.3 5,759,582 + +0.9 5,759,602 + PP_5053 0.11 -1.6 5,759,603 - PP_5053 0.12 -0.4 5,759,766 - PP_5053 0.45 +0.5 5,759,962 + PP_5053 0.85 +0.8 5,759,962 + PP_5053 0.85 +0.3 5,759,962 + PP_5053 0.85 +0.5 5,759,962 + +2.5 5,759,962 + PP_5053 0.85 -1.1 5,759,962 + PP_5053 0.85 +0.3 5,759,962 + PP_5053 0.85 -0.5 5,759,962 + PP_5053 0.85 +0.7 5,759,962 + PP_5053 0.85 +0.7 5,759,962 + PP_5053 0.85 +0.9 5,759,963 - PP_5053 0.86 +0.7 5,759,963 - PP_5053 0.86 +0.1 5,759,963 - PP_5053 0.86 +0.9 5,759,963 - PP_5053 0.86 +0.7 5,759,963 - PP_5053 0.86 +1.3 5,759,963 - PP_5053 0.86 +0.9 5,759,963 - PP_5053 0.86 -0.3 5,760,043 + +0.6 5,760,048 + -0.0 5,760,048 + +0.5 5,760,048 + +0.5 5,760,118 + PP_5054 0.18 +1.7 5,760,119 - PP_5054 0.18 -1.2 5,760,119 - PP_5054 0.18 +0.6 5,760,119 - PP_5054 0.18 -0.9 5,760,269 - PP_5054 0.77 +0.6 5,760,296 - PP_5054 0.88 +0.9 5,760,307 + -0.9 5,760,326 + +0.8 5,760,326 + +1.0 5,760,326 + +0.8 5,760,327 - -0.0 5,760,327 - -0.4 5,760,327 - +0.4 5,760,327 - +1.0 5,760,395 + PP_5055 0.16 +1.6 5,760,395 + PP_5055 0.16 +0.9 5,760,474 + PP_5055 0.35 +1.2 5,760,540 + PP_5055 0.51 +0.5 5,760,541 - PP_5055 0.51 +2.0 5,760,541 - PP_5055 0.51 +0.5 5,760,554 + PP_5055 0.55 +0.5 5,760,555 - PP_5055 0.55 -0.0 5,760,704 + -0.7 5,760,704 + +0.3 5,760,704 + +0.6 5,760,705 - +0.4 5,760,705 - +0.6 5,760,705 - +0.8 5,760,705 - +1.2 5,760,779 + +0.5 5,760,779 + -0.1 5,760,779 + -0.5 5,760,779 + -0.1 5,760,779 + -0.5 5,760,780 - -0.4 5,760,780 - -2.4 5,760,780 - -1.6 5,760,780 - +0.6 5,760,780 - -1.9 5,760,876 + -0.0 5,760,877 - +0.3 5,760,877 - -0.3 5,760,894 + -0.1
Or see this region's nucleotide sequence