Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4800 and PP_4801 are separated by 2 nucleotides PP_4801 and PP_4802 overlap by 4 nucleotides PP_4802 and PP_4803 are separated by 73 nucleotides PP_4803 and PP_4804 are separated by 220 nucleotides
PP_4800: PP_4800 - Lipoyl synthase, at 5,463,448 to 5,464,464
_4800
PP_4801: PP_4801 - Octanoyltransferase, at 5,464,467 to 5,465,117
_4801
PP_4802: PP_4802 - putative lipoate regulatory protein, at 5,465,114 to 5,465,389
_4802
PP_4803: PP_4803 - D-alanyl-D-alanine carboxypeptidase, at 5,465,463 to 5,466,623
_4803
PP_4804: PP_4804 - RlpA-like lipoprotein, at 5,466,844 to 5,467,845
_4804
Position (kb) 5465
5466
5467 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5464.505 kb on - strand at 5465.407 kb on - strand at 5465.462 kb on - strand at 5465.462 kb on - strand at 5465.488 kb on - strand at 5465.682 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.780 kb on - strand, within PP_4803 at 5466.034 kb on + strand at 5466.034 kb on + strand, within PP_4803 at 5466.035 kb on - strand, within PP_4803 at 5466.043 kb on + strand, within PP_4803 at 5466.043 kb on + strand, within PP_4803 at 5466.043 kb on + strand, within PP_4803 at 5466.107 kb on - strand, within PP_4803 at 5466.291 kb on + strand, within PP_4803 at 5466.407 kb on + strand, within PP_4803 at 5466.408 kb on - strand, within PP_4803 at 5466.408 kb on - strand, within PP_4803 at 5466.408 kb on - strand, within PP_4803 at 5466.409 kb on + strand, within PP_4803 at 5466.409 kb on + strand, within PP_4803 at 5466.501 kb on + strand, within PP_4803 at 5466.501 kb on + strand, within PP_4803 at 5466.501 kb on + strand, within PP_4803 at 5466.501 kb on + strand, within PP_4803 at 5466.501 kb on + strand, within PP_4803 at 5466.501 kb on + strand, within PP_4803 at 5466.502 kb on - strand, within PP_4803 at 5466.502 kb on - strand, within PP_4803 at 5466.502 kb on - strand, within PP_4803 at 5466.502 kb on - strand, within PP_4803 at 5466.505 kb on + strand, within PP_4803 at 5466.505 kb on + strand, within PP_4803 at 5466.506 kb on - strand, within PP_4803 at 5466.582 kb on + strand at 5466.582 kb on + strand at 5466.582 kb on + strand at 5466.582 kb on + strand at 5466.696 kb on - strand at 5466.736 kb on + strand at 5466.765 kb on + strand at 5466.765 kb on + strand at 5466.765 kb on + strand at 5466.765 kb on + strand at 5466.765 kb on + strand at 5466.765 kb on + strand at 5466.766 kb on - strand at 5466.766 kb on - strand at 5466.766 kb on - strand at 5466.766 kb on - strand at 5466.766 kb on - strand at 5466.766 kb on - strand at 5466.966 kb on + strand, within PP_4804 at 5466.967 kb on - strand, within PP_4804 at 5467.061 kb on - strand, within PP_4804 at 5467.108 kb on + strand, within PP_4804 at 5467.109 kb on - strand, within PP_4804 at 5467.431 kb on - strand, within PP_4804 at 5467.581 kb on - strand, within PP_4804 at 5467.601 kb on + strand, within PP_4804 at 5467.620 kb on + strand, within PP_4804
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 5,464,505 - -1.2 5,465,407 - +0.3 5,465,462 - +1.3 5,465,462 - +0.4 5,465,488 - -0.6 5,465,682 - PP_4803 0.19 +0.8 5,465,688 - PP_4803 0.19 +1.0 5,465,688 - PP_4803 0.19 +0.9 5,465,688 - PP_4803 0.19 +0.4 5,465,688 - PP_4803 0.19 +1.4 5,465,780 - PP_4803 0.27 +0.1 5,466,034 + +1.4 5,466,034 + PP_4803 0.49 +0.1 5,466,035 - PP_4803 0.49 +0.5 5,466,043 + PP_4803 0.50 -0.0 5,466,043 + PP_4803 0.50 +1.8 5,466,043 + PP_4803 0.50 -0.2 5,466,107 - PP_4803 0.55 +0.7 5,466,291 + PP_4803 0.71 +0.2 5,466,407 + PP_4803 0.81 -0.2 5,466,408 - PP_4803 0.81 +1.3 5,466,408 - PP_4803 0.81 +0.9 5,466,408 - PP_4803 0.81 -0.9 5,466,409 + PP_4803 0.81 +0.4 5,466,409 + PP_4803 0.81 +0.2 5,466,501 + PP_4803 0.89 +0.1 5,466,501 + PP_4803 0.89 -0.9 5,466,501 + PP_4803 0.89 +1.0 5,466,501 + PP_4803 0.89 +0.5 5,466,501 + PP_4803 0.89 +2.1 5,466,501 + PP_4803 0.89 +1.4 5,466,502 - PP_4803 0.89 +0.2 5,466,502 - PP_4803 0.89 +1.2 5,466,502 - PP_4803 0.89 +0.9 5,466,502 - PP_4803 0.89 +1.4 5,466,505 + PP_4803 0.90 +0.9 5,466,505 + PP_4803 0.90 +0.6 5,466,506 - PP_4803 0.90 +0.7 5,466,582 + +0.8 5,466,582 + +0.8 5,466,582 + +1.3 5,466,582 + +0.5 5,466,696 - -0.2 5,466,736 + +1.7 5,466,765 + +0.9 5,466,765 + +0.8 5,466,765 + +0.8 5,466,765 + +0.7 5,466,765 + +0.2 5,466,765 + +1.0 5,466,766 - +0.7 5,466,766 - +0.9 5,466,766 - -0.1 5,466,766 - -0.2 5,466,766 - +1.5 5,466,766 - -0.9 5,466,966 + PP_4804 0.12 -2.7 5,466,967 - PP_4804 0.12 -2.3 5,467,061 - PP_4804 0.22 -3.2 5,467,108 + PP_4804 0.26 -2.4 5,467,109 - PP_4804 0.26 -0.5 5,467,431 - PP_4804 0.59 -2.2 5,467,581 - PP_4804 0.74 -1.1 5,467,601 + PP_4804 0.76 -1.5 5,467,620 + PP_4804 0.77 -2.2
Or see this region's nucleotide sequence