Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4123 and PP_4124 are separated by 132 nucleotides PP_4124 and PP_4125 overlap by 4 nucleotides
PP_4123: PP_4123 - NADH-quinone oxidoreductase subunit F, at 4,659,643 to 4,661,004
_4123
PP_4124: PP_4124 - NADH-quinone oxidoreductase subunit G, at 4,661,137 to 4,663,851
_4124
PP_4125: PP_4125 - NADH-quinone oxidoreductase subunit H, at 4,663,848 to 4,664,855
_4125
Position (kb)
4661
4662
4663
4664 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 4660.373 kb on - strand, within PP_4123 at 4660.501 kb on + strand, within PP_4123 at 4660.857 kb on + strand, within PP_4123 at 4660.913 kb on - strand at 4660.913 kb on - strand at 4661.002 kb on + strand at 4661.145 kb on + strand at 4661.154 kb on - strand at 4661.154 kb on - strand at 4661.196 kb on - strand at 4661.231 kb on - strand at 4661.293 kb on + strand at 4661.293 kb on + strand at 4661.294 kb on - strand at 4661.323 kb on - strand at 4661.323 kb on - strand at 4661.323 kb on - strand at 4661.535 kb on + strand at 4661.536 kb on - strand, within PP_4124 at 4661.614 kb on + strand, within PP_4124 at 4661.617 kb on + strand, within PP_4124 at 4661.617 kb on + strand, within PP_4124 at 4661.617 kb on + strand, within PP_4124 at 4661.617 kb on + strand at 4661.617 kb on + strand, within PP_4124 at 4661.618 kb on - strand, within PP_4124 at 4661.618 kb on - strand at 4661.654 kb on - strand, within PP_4124 at 4661.654 kb on - strand, within PP_4124 at 4661.674 kb on + strand, within PP_4124 at 4661.675 kb on - strand, within PP_4124 at 4661.675 kb on - strand at 4661.750 kb on + strand, within PP_4124 at 4661.873 kb on + strand, within PP_4124 at 4661.873 kb on + strand, within PP_4124 at 4661.951 kb on - strand, within PP_4124 at 4661.951 kb on - strand, within PP_4124 at 4662.045 kb on - strand, within PP_4124 at 4662.054 kb on + strand, within PP_4124 at 4662.100 kb on + strand, within PP_4124 at 4662.131 kb on - strand, within PP_4124 at 4662.136 kb on + strand, within PP_4124 at 4662.136 kb on + strand, within PP_4124 at 4662.136 kb on + strand, within PP_4124 at 4662.464 kb on + strand, within PP_4124 at 4662.465 kb on - strand, within PP_4124 at 4662.783 kb on - strand at 4662.838 kb on + strand, within PP_4124 at 4662.838 kb on + strand, within PP_4124 at 4663.473 kb on - strand, within PP_4124 at 4663.640 kb on - strand at 4663.649 kb on + strand at 4664.313 kb on + strand at 4664.314 kb on - strand, within PP_4125 at 4664.574 kb on + strand, within PP_4125 at 4664.574 kb on + strand, within PP_4125 at 4664.574 kb on + strand, within PP_4125 at 4664.575 kb on - strand, within PP_4125 at 4664.826 kb on + strand at 4664.826 kb on + strand at 4664.826 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 4,660,373 - PP_4123 0.54 +1.4 4,660,501 + PP_4123 0.63 +0.3 4,660,857 + PP_4123 0.89 +1.6 4,660,913 - -1.7 4,660,913 - -0.5 4,661,002 + -1.1 4,661,145 + +1.9 4,661,154 - +1.6 4,661,154 - +3.1 4,661,196 - -1.4 4,661,231 - -0.6 4,661,293 + +0.3 4,661,293 + +2.9 4,661,294 - -2.1 4,661,323 - -1.3 4,661,323 - +0.3 4,661,323 - -1.0 4,661,535 + -0.3 4,661,536 - PP_4124 0.15 +0.5 4,661,614 + PP_4124 0.18 -0.8 4,661,617 + PP_4124 0.18 -0.7 4,661,617 + PP_4124 0.18 +2.4 4,661,617 + PP_4124 0.18 +1.8 4,661,617 + -1.5 4,661,617 + PP_4124 0.18 -0.4 4,661,618 - PP_4124 0.18 +1.5 4,661,618 - +1.3 4,661,654 - PP_4124 0.19 +0.4 4,661,654 - PP_4124 0.19 +1.1 4,661,674 + PP_4124 0.20 +0.7 4,661,675 - PP_4124 0.20 +3.9 4,661,675 - -0.3 4,661,750 + PP_4124 0.23 +0.3 4,661,873 + PP_4124 0.27 +0.2 4,661,873 + PP_4124 0.27 -0.3 4,661,951 - PP_4124 0.30 +1.1 4,661,951 - PP_4124 0.30 -0.6 4,662,045 - PP_4124 0.33 -0.9 4,662,054 + PP_4124 0.34 +0.5 4,662,100 + PP_4124 0.35 -1.9 4,662,131 - PP_4124 0.37 +5.0 4,662,136 + PP_4124 0.37 -0.6 4,662,136 + PP_4124 0.37 -0.2 4,662,136 + PP_4124 0.37 -0.1 4,662,464 + PP_4124 0.49 -0.1 4,662,465 - PP_4124 0.49 +2.2 4,662,783 - +0.5 4,662,838 + PP_4124 0.63 +0.8 4,662,838 + PP_4124 0.63 -2.3 4,663,473 - PP_4124 0.86 +0.9 4,663,640 - -0.9 4,663,649 + +1.1 4,664,313 + -1.9 4,664,314 - PP_4125 0.46 +0.4 4,664,574 + PP_4125 0.72 +0.6 4,664,574 + PP_4125 0.72 +0.4 4,664,574 + PP_4125 0.72 +0.4 4,664,575 - PP_4125 0.72 -0.3 4,664,826 + +0.4 4,664,826 + -1.0 4,664,826 + -0.4
Or see this region's nucleotide sequence