Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2531 and PP_2532 are separated by 142 nucleotides PP_2532 and PP_2533 are separated by 7 nucleotides PP_2533 and PP_2534 are separated by 159 nucleotides
PP_2531: PP_2531 - putative 5-nucleotidase, at 2,876,379 to 2,877,374
_2531
PP_2532: PP_2532 - conserved protein of unknown function, at 2,877,517 to 2,878,002
_2532
PP_2533: PP_2533 - D-isomer specific 2-hydroxyacid dehydrogenase family protein, at 2,878,010 to 2,879,005
_2533
PP_2534: PP_2534 - Transcriptional regulator, LysR family, at 2,879,165 to 2,880,064
_2534
Position (kb)
2878
2879
2880 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2877.034 kb on + strand, within PP_2531 at 2877.041 kb on - strand, within PP_2531 at 2877.088 kb on + strand, within PP_2531 at 2877.292 kb on + strand at 2877.409 kb on + strand at 2877.410 kb on - strand at 2877.410 kb on - strand at 2877.410 kb on - strand at 2877.478 kb on - strand at 2877.500 kb on + strand at 2877.527 kb on + strand at 2877.528 kb on - strand at 2877.528 kb on - strand at 2877.681 kb on + strand, within PP_2532 at 2877.682 kb on - strand, within PP_2532 at 2877.682 kb on - strand, within PP_2532 at 2877.682 kb on - strand, within PP_2532 at 2877.682 kb on - strand, within PP_2532 at 2877.888 kb on + strand, within PP_2532 at 2877.889 kb on - strand, within PP_2532 at 2877.889 kb on - strand, within PP_2532 at 2878.001 kb on - strand at 2878.119 kb on + strand, within PP_2533 at 2878.128 kb on + strand, within PP_2533 at 2878.147 kb on - strand, within PP_2533 at 2878.162 kb on + strand, within PP_2533 at 2878.169 kb on + strand, within PP_2533 at 2878.169 kb on + strand, within PP_2533 at 2878.274 kb on + strand, within PP_2533 at 2878.275 kb on - strand, within PP_2533 at 2878.406 kb on - strand, within PP_2533 at 2878.970 kb on + strand at 2879.044 kb on + strand at 2879.049 kb on + strand at 2879.167 kb on - strand at 2879.180 kb on + strand at 2879.181 kb on - strand at 2879.281 kb on - strand, within PP_2534 at 2879.281 kb on - strand, within PP_2534 at 2879.281 kb on - strand, within PP_2534 at 2879.281 kb on - strand, within PP_2534 at 2879.333 kb on - strand, within PP_2534 at 2879.406 kb on - strand, within PP_2534 at 2879.408 kb on - strand, within PP_2534 at 2879.434 kb on + strand, within PP_2534 at 2879.434 kb on + strand, within PP_2534 at 2879.434 kb on + strand, within PP_2534 at 2879.435 kb on - strand, within PP_2534 at 2879.435 kb on - strand, within PP_2534 at 2879.535 kb on + strand, within PP_2534 at 2879.536 kb on - strand, within PP_2534 at 2879.548 kb on - strand, within PP_2534 at 2879.548 kb on - strand, within PP_2534 at 2879.657 kb on + strand, within PP_2534 at 2879.658 kb on - strand, within PP_2534 at 2879.658 kb on - strand, within PP_2534 at 2879.863 kb on - strand, within PP_2534
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 2,877,034 + PP_2531 0.66 +1.7 2,877,041 - PP_2531 0.66 -0.3 2,877,088 + PP_2531 0.71 -0.0 2,877,292 + +0.2 2,877,409 + +0.4 2,877,410 - -2.7 2,877,410 - -1.7 2,877,410 - +0.5 2,877,478 - -0.1 2,877,500 + -0.0 2,877,527 + +0.2 2,877,528 - +1.1 2,877,528 - -1.4 2,877,681 + PP_2532 0.34 +1.1 2,877,682 - PP_2532 0.34 +0.5 2,877,682 - PP_2532 0.34 -0.5 2,877,682 - PP_2532 0.34 -0.7 2,877,682 - PP_2532 0.34 -0.1 2,877,888 + PP_2532 0.76 -0.9 2,877,889 - PP_2532 0.77 +0.0 2,877,889 - PP_2532 0.77 +0.4 2,878,001 - +0.3 2,878,119 + PP_2533 0.11 +0.5 2,878,128 + PP_2533 0.12 +0.4 2,878,147 - PP_2533 0.14 +1.2 2,878,162 + PP_2533 0.15 -3.0 2,878,169 + PP_2533 0.16 +0.5 2,878,169 + PP_2533 0.16 +0.7 2,878,274 + PP_2533 0.27 +0.6 2,878,275 - PP_2533 0.27 +0.1 2,878,406 - PP_2533 0.40 +1.9 2,878,970 + +0.3 2,879,044 + -0.4 2,879,049 + -0.8 2,879,167 - -0.6 2,879,180 + -1.0 2,879,181 - -0.5 2,879,281 - PP_2534 0.13 +1.0 2,879,281 - PP_2534 0.13 +0.0 2,879,281 - PP_2534 0.13 -1.0 2,879,281 - PP_2534 0.13 +0.9 2,879,333 - PP_2534 0.19 -1.3 2,879,406 - PP_2534 0.27 -0.3 2,879,408 - PP_2534 0.27 +0.0 2,879,434 + PP_2534 0.30 -0.8 2,879,434 + PP_2534 0.30 +0.0 2,879,434 + PP_2534 0.30 +0.2 2,879,435 - PP_2534 0.30 -0.9 2,879,435 - PP_2534 0.30 +0.9 2,879,535 + PP_2534 0.41 +0.2 2,879,536 - PP_2534 0.41 -2.1 2,879,548 - PP_2534 0.43 -0.2 2,879,548 - PP_2534 0.43 +0.2 2,879,657 + PP_2534 0.55 -0.0 2,879,658 - PP_2534 0.55 +0.8 2,879,658 - PP_2534 0.55 +0.0 2,879,863 - PP_2534 0.78 -2.0
Or see this region's nucleotide sequence