Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1728 and PP_1729 are separated by 38 nucleotides PP_1729 and PP_1730 are separated by 104 nucleotides
PP_1728: PP_1728 - conserved membrane protein of unknown function, at 1,927,938 to 1,930,091
_1728
PP_1729: PP_1729 - conserved protein of unknown function, at 1,930,130 to 1,930,333
_1729
PP_1730: PP_1730 - Probable M18 family aminopeptidase 2, at 1,930,438 to 1,931,727
_1730
Position (kb)
1930
1931 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1929.198 kb on - strand, within PP_1728 at 1929.422 kb on + strand at 1929.422 kb on + strand, within PP_1728 at 1929.496 kb on + strand, within PP_1728 at 1929.497 kb on - strand, within PP_1728 at 1929.641 kb on - strand, within PP_1728 at 1930.030 kb on - strand at 1930.123 kb on + strand at 1930.123 kb on + strand at 1930.131 kb on + strand at 1930.131 kb on + strand at 1930.131 kb on + strand at 1930.132 kb on - strand at 1930.132 kb on - strand at 1930.177 kb on + strand, within PP_1729 at 1930.206 kb on - strand, within PP_1729 at 1930.434 kb on + strand at 1930.435 kb on - strand at 1930.435 kb on - strand at 1930.460 kb on + strand at 1930.460 kb on + strand at 1930.460 kb on + strand at 1930.474 kb on + strand at 1930.474 kb on + strand at 1930.475 kb on - strand at 1930.475 kb on - strand at 1930.645 kb on + strand, within PP_1730 at 1930.847 kb on + strand, within PP_1730 at 1930.848 kb on - strand, within PP_1730 at 1931.084 kb on + strand, within PP_1730 at 1931.084 kb on + strand, within PP_1730 at 1931.085 kb on - strand, within PP_1730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 1,929,198 - PP_1728 0.58 -1.6 1,929,422 + +2.3 1,929,422 + PP_1728 0.69 +0.7 1,929,496 + PP_1728 0.72 -0.6 1,929,497 - PP_1728 0.72 +0.7 1,929,641 - PP_1728 0.79 -0.7 1,930,030 - -0.6 1,930,123 + +0.3 1,930,123 + +1.3 1,930,131 + -1.4 1,930,131 + -0.7 1,930,131 + -1.8 1,930,132 - +0.9 1,930,132 - -1.4 1,930,177 + PP_1729 0.23 -0.7 1,930,206 - PP_1729 0.37 -0.5 1,930,434 + +0.6 1,930,435 - -0.4 1,930,435 - +1.9 1,930,460 + +0.1 1,930,460 + -0.7 1,930,460 + -0.1 1,930,474 + -0.8 1,930,474 + -0.0 1,930,475 - -0.4 1,930,475 - -0.1 1,930,645 + PP_1730 0.16 -0.2 1,930,847 + PP_1730 0.32 +1.0 1,930,848 - PP_1730 0.32 -0.8 1,931,084 + PP_1730 0.50 -1.1 1,931,084 + PP_1730 0.50 +1.1 1,931,085 - PP_1730 0.50 +0.7
Or see this region's nucleotide sequence