Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4799 and PP_4800 are separated by 98 nucleotides PP_4800 and PP_4801 are separated by 2 nucleotides PP_4801 and PP_4802 overlap by 4 nucleotides PP_4802 and PP_4803 are separated by 73 nucleotides
PP_4799: PP_4799 - putative Muramoyltetrapeptide carboxypeptidase, at 5,462,411 to 5,463,349
_4799
PP_4800: PP_4800 - Lipoyl synthase, at 5,463,448 to 5,464,464
_4800
PP_4801: PP_4801 - Octanoyltransferase, at 5,464,467 to 5,465,117
_4801
PP_4802: PP_4802 - putative lipoate regulatory protein, at 5,465,114 to 5,465,389
_4802
PP_4803: PP_4803 - D-alanyl-D-alanine carboxypeptidase, at 5,465,463 to 5,466,623
_4803
Position (kb) 5463
5464
5465 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5462.492 kb on + strand at 5462.492 kb on + strand at 5462.493 kb on - strand at 5462.493 kb on - strand at 5462.493 kb on - strand at 5462.558 kb on - strand, within PP_4799 at 5462.558 kb on - strand, within PP_4799 at 5462.558 kb on - strand, within PP_4799 at 5462.590 kb on + strand, within PP_4799 at 5462.591 kb on - strand, within PP_4799 at 5462.973 kb on + strand, within PP_4799 at 5462.983 kb on + strand, within PP_4799 at 5462.984 kb on - strand, within PP_4799 at 5463.202 kb on + strand, within PP_4799 at 5463.202 kb on + strand, within PP_4799 at 5463.437 kb on + strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5464.505 kb on - strand at 5465.407 kb on - strand at 5465.462 kb on - strand at 5465.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 5,462,492 + -2.2 5,462,492 + -3.1 5,462,493 - -1.1 5,462,493 - -0.3 5,462,493 - -3.1 5,462,558 - PP_4799 0.16 -2.3 5,462,558 - PP_4799 0.16 -2.5 5,462,558 - PP_4799 0.16 -1.6 5,462,590 + PP_4799 0.19 -1.5 5,462,591 - PP_4799 0.19 -2.2 5,462,973 + PP_4799 0.60 -2.6 5,462,983 + PP_4799 0.61 -2.1 5,462,984 - PP_4799 0.61 -1.6 5,463,202 + PP_4799 0.84 -1.7 5,463,202 + PP_4799 0.84 -3.4 5,463,437 + +0.4 5,463,445 - +0.3 5,463,445 - -3.9 5,463,445 - +0.2 5,463,445 - +0.4 5,463,445 - -0.4 5,463,445 - +0.4 5,464,505 - +0.4 5,465,407 - -0.2 5,465,462 - -0.2 5,465,462 - -4.1
Or see this region's nucleotide sequence