Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4798 and PP_4799 are separated by 33 nucleotides PP_4799 and PP_4800 are separated by 98 nucleotides
PP_4798: PP_4798 - putative Membrane-bound lytic murein transglycosylase, at 5,461,061 to 5,462,377
_4798
PP_4799: PP_4799 - putative Muramoyltetrapeptide carboxypeptidase, at 5,462,411 to 5,463,349
_4799
PP_4800: PP_4800 - Lipoyl synthase, at 5,463,448 to 5,464,464
_4800
Position (kb)
5462
5463
5464 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5461.503 kb on - strand, within PP_4798 at 5461.748 kb on + strand, within PP_4798 at 5461.748 kb on + strand, within PP_4798 at 5461.749 kb on - strand, within PP_4798 at 5461.835 kb on + strand, within PP_4798 at 5461.835 kb on + strand, within PP_4798 at 5461.835 kb on + strand, within PP_4798 at 5461.836 kb on - strand, within PP_4798 at 5461.885 kb on - strand, within PP_4798 at 5461.885 kb on - strand, within PP_4798 at 5461.885 kb on - strand, within PP_4798 at 5461.999 kb on + strand, within PP_4798 at 5462.091 kb on - strand, within PP_4798 at 5462.091 kb on - strand, within PP_4798 at 5462.108 kb on + strand, within PP_4798 at 5462.108 kb on + strand, within PP_4798 at 5462.109 kb on - strand, within PP_4798 at 5462.109 kb on - strand, within PP_4798 at 5462.255 kb on - strand at 5462.281 kb on + strand at 5462.375 kb on + strand at 5462.376 kb on - strand at 5462.376 kb on - strand at 5462.492 kb on + strand at 5462.492 kb on + strand at 5462.493 kb on - strand at 5462.493 kb on - strand at 5462.493 kb on - strand at 5462.558 kb on - strand, within PP_4799 at 5462.558 kb on - strand, within PP_4799 at 5462.558 kb on - strand, within PP_4799 at 5462.590 kb on + strand, within PP_4799 at 5462.591 kb on - strand, within PP_4799 at 5462.973 kb on + strand, within PP_4799 at 5462.983 kb on + strand, within PP_4799 at 5462.984 kb on - strand, within PP_4799 at 5463.202 kb on + strand, within PP_4799 at 5463.202 kb on + strand, within PP_4799 at 5463.437 kb on + strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand at 5463.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 5,461,503 - PP_4798 0.34 +1.8 5,461,748 + PP_4798 0.52 -0.5 5,461,748 + PP_4798 0.52 -0.7 5,461,749 - PP_4798 0.52 +0.1 5,461,835 + PP_4798 0.59 +0.7 5,461,835 + PP_4798 0.59 -1.4 5,461,835 + PP_4798 0.59 +1.7 5,461,836 - PP_4798 0.59 +0.2 5,461,885 - PP_4798 0.63 +1.0 5,461,885 - PP_4798 0.63 -2.1 5,461,885 - PP_4798 0.63 -2.2 5,461,999 + PP_4798 0.71 -2.0 5,462,091 - PP_4798 0.78 +0.3 5,462,091 - PP_4798 0.78 +0.5 5,462,108 + PP_4798 0.79 +0.4 5,462,108 + PP_4798 0.79 -1.8 5,462,109 - PP_4798 0.80 -1.4 5,462,109 - PP_4798 0.80 -3.3 5,462,255 - -1.5 5,462,281 + -0.8 5,462,375 + +0.8 5,462,376 - -0.4 5,462,376 - +1.2 5,462,492 + -2.2 5,462,492 + -3.1 5,462,493 - -1.1 5,462,493 - -0.3 5,462,493 - -3.1 5,462,558 - PP_4799 0.16 -2.3 5,462,558 - PP_4799 0.16 -2.5 5,462,558 - PP_4799 0.16 -1.6 5,462,590 + PP_4799 0.19 -1.5 5,462,591 - PP_4799 0.19 -2.2 5,462,973 + PP_4799 0.60 -2.6 5,462,983 + PP_4799 0.61 -2.1 5,462,984 - PP_4799 0.61 -1.6 5,463,202 + PP_4799 0.84 -1.7 5,463,202 + PP_4799 0.84 -3.4 5,463,437 + +0.4 5,463,445 - +0.3 5,463,445 - -3.9 5,463,445 - +0.2 5,463,445 - +0.4 5,463,445 - -0.4 5,463,445 - +0.4
Or see this region's nucleotide sequence