Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2853 and PP_2854 are separated by 46 nucleotides PP_2854 and PP_2855 are separated by 45 nucleotides PP_2855 and PP_2856 are separated by 61 nucleotides
PP_2853: PP_2853 - conserved exported protein of unknown function, at 3,255,873 to 3,257,153
_2853
PP_2854: PP_2854 - conserved exported protein of unknown function, at 3,257,200 to 3,257,952
_2854
PP_2855: PP_2855 - conserved exported protein of unknown function, at 3,257,998 to 3,258,678
_2855
PP_2856: PP_2856 - conserved exported protein of unknown function, at 3,258,740 to 3,260,191
_2856
Position (kb)
3257
3258
3259 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3257.024 kb on - strand, within PP_2853 at 3257.150 kb on - strand at 3257.175 kb on + strand at 3257.176 kb on - strand at 3257.176 kb on - strand at 3257.196 kb on + strand at 3257.488 kb on + strand, within PP_2854 at 3257.541 kb on + strand, within PP_2854 at 3257.556 kb on - strand, within PP_2854 at 3257.705 kb on + strand, within PP_2854 at 3257.705 kb on + strand, within PP_2854 at 3257.706 kb on - strand at 3257.706 kb on - strand, within PP_2854 at 3257.788 kb on - strand, within PP_2854 at 3257.788 kb on - strand, within PP_2854 at 3257.834 kb on + strand, within PP_2854 at 3257.953 kb on - strand at 3257.987 kb on + strand at 3258.188 kb on + strand, within PP_2855 at 3258.189 kb on - strand, within PP_2855 at 3258.189 kb on - strand, within PP_2855 at 3258.221 kb on + strand, within PP_2855 at 3258.221 kb on + strand, within PP_2855 at 3258.222 kb on - strand, within PP_2855 at 3258.222 kb on - strand, within PP_2855 at 3258.222 kb on - strand, within PP_2855 at 3258.266 kb on + strand, within PP_2855 at 3258.289 kb on + strand, within PP_2855 at 3258.473 kb on + strand, within PP_2855 at 3258.474 kb on - strand, within PP_2855 at 3258.692 kb on - strand at 3258.801 kb on - strand at 3258.862 kb on + strand at 3258.910 kb on + strand, within PP_2856 at 3258.911 kb on - strand, within PP_2856 at 3258.911 kb on - strand, within PP_2856 at 3258.911 kb on - strand, within PP_2856 at 3258.911 kb on - strand, within PP_2856 at 3258.911 kb on - strand, within PP_2856 at 3258.925 kb on - strand, within PP_2856 at 3259.021 kb on - strand, within PP_2856 at 3259.045 kb on - strand, within PP_2856 at 3259.092 kb on + strand, within PP_2856 at 3259.114 kb on - strand, within PP_2856 at 3259.129 kb on - strand at 3259.231 kb on + strand, within PP_2856 at 3259.231 kb on + strand, within PP_2856 at 3259.232 kb on - strand, within PP_2856 at 3259.238 kb on - strand, within PP_2856 at 3259.532 kb on - strand, within PP_2856 at 3259.548 kb on + strand, within PP_2856 at 3259.549 kb on - strand, within PP_2856 at 3259.571 kb on + strand, within PP_2856
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,257,024 - PP_2853 0.90 -1.2 3,257,150 - -0.2 3,257,175 + +0.5 3,257,176 - +0.9 3,257,176 - +0.6 3,257,196 + -1.8 3,257,488 + PP_2854 0.38 -0.6 3,257,541 + PP_2854 0.45 -0.5 3,257,556 - PP_2854 0.47 +0.2 3,257,705 + PP_2854 0.67 +0.3 3,257,705 + PP_2854 0.67 -0.1 3,257,706 - +0.5 3,257,706 - PP_2854 0.67 -0.0 3,257,788 - PP_2854 0.78 +0.8 3,257,788 - PP_2854 0.78 +0.8 3,257,834 + PP_2854 0.84 +1.3 3,257,953 - -0.7 3,257,987 + -0.1 3,258,188 + PP_2855 0.28 -3.6 3,258,189 - PP_2855 0.28 -1.6 3,258,189 - PP_2855 0.28 -0.2 3,258,221 + PP_2855 0.33 -2.3 3,258,221 + PP_2855 0.33 +0.7 3,258,222 - PP_2855 0.33 -0.8 3,258,222 - PP_2855 0.33 -0.0 3,258,222 - PP_2855 0.33 -0.8 3,258,266 + PP_2855 0.39 -1.4 3,258,289 + PP_2855 0.43 +0.4 3,258,473 + PP_2855 0.70 -0.1 3,258,474 - PP_2855 0.70 -1.6 3,258,692 - -1.4 3,258,801 - -0.2 3,258,862 + -1.1 3,258,910 + PP_2856 0.12 -0.4 3,258,911 - PP_2856 0.12 +0.1 3,258,911 - PP_2856 0.12 -1.4 3,258,911 - PP_2856 0.12 +0.3 3,258,911 - PP_2856 0.12 +0.1 3,258,911 - PP_2856 0.12 +0.5 3,258,925 - PP_2856 0.13 -0.8 3,259,021 - PP_2856 0.19 -1.3 3,259,045 - PP_2856 0.21 -2.0 3,259,092 + PP_2856 0.24 -1.1 3,259,114 - PP_2856 0.26 +2.6 3,259,129 - +1.0 3,259,231 + PP_2856 0.34 +1.8 3,259,231 + PP_2856 0.34 +0.4 3,259,232 - PP_2856 0.34 -1.2 3,259,238 - PP_2856 0.34 +1.2 3,259,532 - PP_2856 0.55 -1.6 3,259,548 + PP_2856 0.56 -4.2 3,259,549 - PP_2856 0.56 -3.7 3,259,571 + PP_2856 0.57 +0.2
Or see this region's nucleotide sequence