Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2709 and PP_2710 are separated by 8 nucleotides PP_2710 and PP_2711 overlap by 14 nucleotides PP_2711 and PP_2712 overlap by 4 nucleotides PP_2712 and PP_2713 are separated by 2 nucleotides
PP_2709: PP_2709 - putative Long-chain-fatty-acid--CoA ligase, at 3,099,222 to 3,100,691
_2709
PP_2710: PP_2710 - conserved protein of unknown function, at 3,100,700 to 3,101,380
_2710
PP_2711: PP_2711 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 3,101,367 to 3,102,173
_2711
PP_2712: PP_2712 - conserved exported protein of unknown function, at 3,102,170 to 3,102,811
_2712
PP_2713: PP_2713 - DNA-binding response regulator, at 3,102,814 to 3,103,476
_2713
Position (kb)
3101
3102
3103 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3100.396 kb on + strand, within PP_2709 at 3100.396 kb on + strand, within PP_2709 at 3100.396 kb on + strand, within PP_2709 at 3100.397 kb on - strand, within PP_2709 at 3100.525 kb on + strand, within PP_2709 at 3100.595 kb on + strand at 3100.595 kb on + strand at 3100.808 kb on + strand, within PP_2710 at 3100.808 kb on + strand, within PP_2710 at 3100.809 kb on - strand, within PP_2710 at 3100.832 kb on + strand, within PP_2710 at 3100.833 kb on - strand, within PP_2710 at 3100.925 kb on + strand, within PP_2710 at 3100.925 kb on + strand, within PP_2710 at 3100.926 kb on - strand, within PP_2710 at 3100.941 kb on - strand, within PP_2710 at 3100.941 kb on - strand, within PP_2710 at 3101.045 kb on + strand, within PP_2710 at 3101.189 kb on + strand, within PP_2710 at 3101.234 kb on + strand, within PP_2710 at 3101.235 kb on - strand, within PP_2710 at 3101.417 kb on - strand at 3101.417 kb on - strand at 3101.417 kb on - strand at 3101.480 kb on + strand, within PP_2711 at 3101.664 kb on + strand, within PP_2711 at 3101.665 kb on - strand, within PP_2711 at 3101.680 kb on + strand, within PP_2711 at 3101.680 kb on + strand, within PP_2711 at 3101.680 kb on + strand, within PP_2711 at 3101.681 kb on - strand, within PP_2711 at 3101.781 kb on + strand, within PP_2711 at 3101.896 kb on - strand, within PP_2711 at 3101.972 kb on + strand, within PP_2711 at 3102.040 kb on - strand, within PP_2711 at 3102.072 kb on - strand, within PP_2711 at 3102.138 kb on + strand at 3102.139 kb on - strand at 3102.141 kb on - strand at 3102.284 kb on + strand, within PP_2712 at 3102.518 kb on + strand, within PP_2712 at 3102.539 kb on + strand, within PP_2712 at 3102.540 kb on - strand at 3102.540 kb on - strand, within PP_2712 at 3102.644 kb on + strand, within PP_2712 at 3102.691 kb on + strand, within PP_2712 at 3103.105 kb on + strand, within PP_2713 at 3103.106 kb on - strand, within PP_2713
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,100,396 + PP_2709 0.80 -0.1 3,100,396 + PP_2709 0.80 -1.0 3,100,396 + PP_2709 0.80 -2.4 3,100,397 - PP_2709 0.80 -0.2 3,100,525 + PP_2709 0.89 +1.0 3,100,595 + -2.7 3,100,595 + -0.4 3,100,808 + PP_2710 0.16 +0.7 3,100,808 + PP_2710 0.16 +1.2 3,100,809 - PP_2710 0.16 -0.5 3,100,832 + PP_2710 0.19 -0.5 3,100,833 - PP_2710 0.20 +1.1 3,100,925 + PP_2710 0.33 -2.7 3,100,925 + PP_2710 0.33 +0.2 3,100,926 - PP_2710 0.33 +0.1 3,100,941 - PP_2710 0.35 +0.8 3,100,941 - PP_2710 0.35 +0.1 3,101,045 + PP_2710 0.51 -0.9 3,101,189 + PP_2710 0.72 +0.3 3,101,234 + PP_2710 0.78 -1.6 3,101,235 - PP_2710 0.79 +0.0 3,101,417 - -1.9 3,101,417 - +1.6 3,101,417 - -1.5 3,101,480 + PP_2711 0.14 -0.7 3,101,664 + PP_2711 0.37 -0.5 3,101,665 - PP_2711 0.37 -0.5 3,101,680 + PP_2711 0.39 +0.5 3,101,680 + PP_2711 0.39 -4.1 3,101,680 + PP_2711 0.39 -0.4 3,101,681 - PP_2711 0.39 -0.9 3,101,781 + PP_2711 0.51 -0.4 3,101,896 - PP_2711 0.66 -1.3 3,101,972 + PP_2711 0.75 -1.2 3,102,040 - PP_2711 0.83 +0.3 3,102,072 - PP_2711 0.87 -1.6 3,102,138 + -0.8 3,102,139 - +0.5 3,102,141 - -0.8 3,102,284 + PP_2712 0.18 -1.8 3,102,518 + PP_2712 0.54 +0.2 3,102,539 + PP_2712 0.57 +0.2 3,102,540 - +0.4 3,102,540 - PP_2712 0.58 +0.7 3,102,644 + PP_2712 0.74 +0.7 3,102,691 + PP_2712 0.81 +0.7 3,103,105 + PP_2713 0.44 +2.0 3,103,106 - PP_2713 0.44 -0.0
Or see this region's nucleotide sequence