Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1475 and PP_1476 are separated by 130 nucleotides PP_1476 and PP_1477 are separated by 8 nucleotides
PP_1475: PP_1475 - CAIB/BAIF family protein, at 1,679,601 to 1,680,800
_1475
PP_1476: PP_1476 - conserved protein of unknown function, at 1,680,931 to 1,681,473
_1476
PP_1477: PP_1477 - ssDNA-specific exonuclease RecJ, at 1,681,482 to 1,683,191
_1477
Position (kb)
1680
1681
1682 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1680.148 kb on - strand, within PP_1475 at 1680.190 kb on + strand, within PP_1475 at 1680.190 kb on + strand, within PP_1475 at 1680.344 kb on - strand, within PP_1475 at 1680.344 kb on - strand, within PP_1475 at 1680.638 kb on - strand, within PP_1475 at 1680.767 kb on + strand at 1680.768 kb on - strand at 1680.893 kb on - strand at 1680.953 kb on - strand at 1681.069 kb on + strand, within PP_1476 at 1681.069 kb on + strand, within PP_1476 at 1681.070 kb on - strand, within PP_1476 at 1681.082 kb on - strand, within PP_1476 at 1681.250 kb on - strand, within PP_1476 at 1681.276 kb on - strand, within PP_1476 at 1681.369 kb on - strand, within PP_1476 at 1681.369 kb on - strand, within PP_1476 at 1681.402 kb on - strand, within PP_1476 at 1681.402 kb on - strand at 1681.620 kb on + strand at 1681.783 kb on - strand, within PP_1477 at 1681.783 kb on - strand, within PP_1477 at 1681.813 kb on - strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.104 kb on - strand, within PP_1477
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 1,680,148 - PP_1475 0.46 -0.4 1,680,190 + PP_1475 0.49 -4.1 1,680,190 + PP_1475 0.49 -0.6 1,680,344 - PP_1475 0.62 -0.4 1,680,344 - PP_1475 0.62 -1.6 1,680,638 - PP_1475 0.86 -2.0 1,680,767 + -0.8 1,680,768 - -1.3 1,680,893 - +0.2 1,680,953 - +0.5 1,681,069 + PP_1476 0.25 -0.3 1,681,069 + PP_1476 0.25 +0.4 1,681,070 - PP_1476 0.26 -4.9 1,681,082 - PP_1476 0.28 -0.6 1,681,250 - PP_1476 0.59 -0.9 1,681,276 - PP_1476 0.64 -1.4 1,681,369 - PP_1476 0.81 -0.6 1,681,369 - PP_1476 0.81 -1.6 1,681,402 - PP_1476 0.87 -0.9 1,681,402 - +1.6 1,681,620 + +0.2 1,681,783 - PP_1477 0.18 -0.1 1,681,783 - PP_1477 0.18 -1.1 1,681,813 - PP_1477 0.19 +0.5 1,682,058 + PP_1477 0.34 +1.2 1,682,058 + PP_1477 0.34 +1.0 1,682,058 + PP_1477 0.34 -2.2 1,682,059 - PP_1477 0.34 +0.8 1,682,059 - PP_1477 0.34 -3.8 1,682,059 - PP_1477 0.34 -0.7 1,682,104 - PP_1477 0.36 -2.2
Or see this region's nucleotide sequence