Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0556 and PP_0557 are separated by 283 nucleotides PP_0557 and PP_0558 are separated by 90 nucleotides
PP_0556: PP_0556 - Acetoin catabolism protein, at 645,039 to 646,094
_0556
PP_0557: PP_0557 - Acetoin catabolism regulatory protein, at 646,378 to 648,231
_0557
PP_0558: PP_0558 - acetyl-CoA carboxylase - biotin carboxylase subunit, at 648,322 to 649,677
_0558
Position (kb) 646
647
648
649 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 645.561 kb on + strand, within PP_0556 at 645.561 kb on + strand, within PP_0556 at 645.562 kb on - strand, within PP_0556 at 646.107 kb on - strand at 646.175 kb on + strand at 646.331 kb on - strand at 646.331 kb on - strand at 646.348 kb on - strand at 646.504 kb on + strand at 646.648 kb on + strand, within PP_0557 at 646.649 kb on - strand, within PP_0557 at 646.649 kb on - strand, within PP_0557 at 646.649 kb on - strand, within PP_0557 at 646.662 kb on + strand, within PP_0557 at 646.738 kb on + strand, within PP_0557 at 646.738 kb on + strand, within PP_0557 at 646.739 kb on - strand, within PP_0557 at 646.913 kb on + strand, within PP_0557 at 646.913 kb on + strand, within PP_0557 at 646.913 kb on + strand, within PP_0557 at 646.913 kb on + strand, within PP_0557 at 646.914 kb on - strand, within PP_0557 at 646.914 kb on - strand, within PP_0557 at 646.914 kb on - strand, within PP_0557 at 646.914 kb on - strand, within PP_0557 at 647.065 kb on + strand, within PP_0557 at 647.066 kb on - strand, within PP_0557 at 647.066 kb on - strand, within PP_0557 at 647.086 kb on + strand, within PP_0557 at 647.086 kb on + strand, within PP_0557 at 647.087 kb on - strand, within PP_0557 at 647.181 kb on + strand, within PP_0557 at 647.181 kb on + strand, within PP_0557 at 647.181 kb on + strand, within PP_0557 at 647.181 kb on + strand, within PP_0557 at 647.182 kb on - strand, within PP_0557 at 647.263 kb on + strand, within PP_0557 at 647.264 kb on - strand, within PP_0557 at 647.264 kb on - strand, within PP_0557 at 647.788 kb on + strand, within PP_0557 at 647.788 kb on + strand, within PP_0557 at 647.788 kb on + strand, within PP_0557 at 647.788 kb on + strand, within PP_0557 at 647.987 kb on - strand, within PP_0557 at 648.305 kb on + strand at 648.315 kb on + strand at 648.315 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 645,561 + PP_0556 0.49 +0.1 645,561 + PP_0556 0.49 +0.2 645,562 - PP_0556 0.50 +0.2 646,107 - -1.3 646,175 + +0.4 646,331 - +0.3 646,331 - -1.4 646,348 - -0.3 646,504 + -0.5 646,648 + PP_0557 0.15 -1.4 646,649 - PP_0557 0.15 +0.3 646,649 - PP_0557 0.15 -0.4 646,649 - PP_0557 0.15 +0.0 646,662 + PP_0557 0.15 -0.2 646,738 + PP_0557 0.19 -0.4 646,738 + PP_0557 0.19 -0.5 646,739 - PP_0557 0.19 -0.8 646,913 + PP_0557 0.29 -1.3 646,913 + PP_0557 0.29 -0.2 646,913 + PP_0557 0.29 -1.0 646,913 + PP_0557 0.29 -0.2 646,914 - PP_0557 0.29 +0.2 646,914 - PP_0557 0.29 +2.1 646,914 - PP_0557 0.29 -0.9 646,914 - PP_0557 0.29 +0.4 647,065 + PP_0557 0.37 +0.1 647,066 - PP_0557 0.37 -1.9 647,066 - PP_0557 0.37 -1.6 647,086 + PP_0557 0.38 -0.4 647,086 + PP_0557 0.38 -3.5 647,087 - PP_0557 0.38 -2.4 647,181 + PP_0557 0.43 -1.1 647,181 + PP_0557 0.43 -0.2 647,181 + PP_0557 0.43 -0.0 647,181 + PP_0557 0.43 +1.0 647,182 - PP_0557 0.43 -0.9 647,263 + PP_0557 0.48 -1.3 647,264 - PP_0557 0.48 -1.3 647,264 - PP_0557 0.48 -2.6 647,788 + PP_0557 0.76 -1.5 647,788 + PP_0557 0.76 -0.4 647,788 + PP_0557 0.76 +0.7 647,788 + PP_0557 0.76 -0.0 647,987 - PP_0557 0.87 +0.6 648,305 + -0.3 648,315 + +0.2 648,315 + -0.1
Or see this region's nucleotide sequence