Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0405 and PP_0406 overlap by 4 nucleotides PP_0406 and PP_0407 are separated by 1 nucleotides PP_0407 and PP_0408 are separated by 27 nucleotides
PP_0405: PP_0405 - conserved protein of unknown function, at 492,551 to 493,570
_0405
PP_0406: PP_0406 - Nucleotidyltransferase family protein, at 493,567 to 494,238
_0406
PP_0407: PP_0407 - DnaJ-like protein DjlA, at 494,240 to 495,007
_0407
PP_0408: PP_0408 - conserved exported protein of unknown function, at 495,035 to 496,033
_0408
Position (kb) 494
495
496 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.241 kb on - strand, within PP_0405 at 493.241 kb on - strand, within PP_0405 at 493.454 kb on + strand, within PP_0405 at 493.455 kb on - strand, within PP_0405 at 493.484 kb on + strand at 493.485 kb on - strand at 493.485 kb on - strand at 493.486 kb on + strand at 493.487 kb on - strand at 493.487 kb on - strand at 493.488 kb on + strand at 493.488 kb on + strand at 493.488 kb on + strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.673 kb on - strand, within PP_0406 at 493.901 kb on - strand, within PP_0406 at 494.147 kb on + strand, within PP_0406 at 494.148 kb on - strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.473 kb on + strand, within PP_0407 at 494.473 kb on + strand, within PP_0407 at 494.626 kb on + strand, within PP_0407 at 494.801 kb on + strand, within PP_0407 at 494.802 kb on - strand, within PP_0407 at 494.802 kb on - strand, within PP_0407 at 494.868 kb on - strand, within PP_0407 at 494.912 kb on - strand, within PP_0407 at 494.912 kb on - strand, within PP_0407 at 494.975 kb on + strand at 494.975 kb on + strand at 494.976 kb on - strand at 494.976 kb on - strand at 495.139 kb on + strand, within PP_0408 at 495.139 kb on + strand, within PP_0408 at 495.207 kb on + strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.214 kb on + strand, within PP_0408 at 495.214 kb on + strand, within PP_0408 at 495.308 kb on + strand, within PP_0408 at 495.336 kb on + strand, within PP_0408 at 495.337 kb on - strand, within PP_0408 at 495.351 kb on + strand, within PP_0408 at 495.361 kb on + strand, within PP_0408 at 495.362 kb on - strand, within PP_0408 at 495.378 kb on - strand, within PP_0408
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 493,240 + PP_0405 0.68 -0.9 493,240 + PP_0405 0.68 -3.4 493,240 + PP_0405 0.68 +0.4 493,240 + PP_0405 0.68 -3.4 493,240 + PP_0405 0.68 -2.1 493,240 + PP_0405 0.68 +0.0 493,241 - PP_0405 0.68 -1.4 493,241 - PP_0405 0.68 -0.4 493,454 + PP_0405 0.89 +0.3 493,455 - PP_0405 0.89 -0.3 493,484 + -0.2 493,485 - +2.6 493,485 - -1.6 493,486 + +0.1 493,487 - -3.1 493,487 - -1.0 493,488 + +1.5 493,488 + -0.2 493,488 + -0.1 493,489 - -2.2 493,489 - -2.0 493,489 - +0.7 493,489 - +1.1 493,489 - -1.7 493,489 - -1.0 493,673 - PP_0406 0.16 -1.7 493,901 - PP_0406 0.50 +0.4 494,147 + PP_0406 0.86 -0.1 494,148 - PP_0406 0.86 -0.5 494,161 + PP_0406 0.88 -0.3 494,161 + PP_0406 0.88 +1.2 494,162 - PP_0406 0.89 -1.4 494,162 - PP_0406 0.89 -0.3 494,162 - PP_0406 0.89 +0.1 494,473 + PP_0407 0.30 -2.0 494,473 + PP_0407 0.30 -1.6 494,626 + PP_0407 0.50 -1.1 494,801 + PP_0407 0.73 -1.3 494,802 - PP_0407 0.73 -0.8 494,802 - PP_0407 0.73 -0.5 494,868 - PP_0407 0.82 -0.7 494,912 - PP_0407 0.88 -0.4 494,912 - PP_0407 0.88 -0.5 494,975 + -0.5 494,975 + +0.7 494,976 - +0.3 494,976 - -1.7 495,139 + PP_0408 0.10 -0.1 495,139 + PP_0408 0.10 -0.4 495,207 + PP_0408 0.17 +0.2 495,208 - PP_0408 0.17 +0.2 495,208 - PP_0408 0.17 -1.7 495,208 - PP_0408 0.17 +0.5 495,208 - PP_0408 0.17 -2.9 495,210 + PP_0408 0.18 -0.7 495,210 + PP_0408 0.18 +0.1 495,210 + PP_0408 0.18 +0.4 495,210 + PP_0408 0.18 +1.0 495,210 + PP_0408 0.18 -0.0 495,210 + PP_0408 0.18 +0.3 495,210 + PP_0408 0.18 -1.8 495,210 + PP_0408 0.18 -2.3 495,211 - PP_0408 0.18 -0.2 495,211 - PP_0408 0.18 -1.2 495,211 - PP_0408 0.18 +0.7 495,211 - PP_0408 0.18 -2.4 495,211 - PP_0408 0.18 -0.1 495,214 + PP_0408 0.18 -0.2 495,214 + PP_0408 0.18 +0.3 495,308 + PP_0408 0.27 +0.1 495,336 + PP_0408 0.30 +0.1 495,337 - PP_0408 0.30 -0.5 495,351 + PP_0408 0.32 -0.9 495,361 + PP_0408 0.33 +0.6 495,362 - PP_0408 0.33 +0.0 495,378 - PP_0408 0.34 -2.9
Or see this region's nucleotide sequence