Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3189 and PP_3190 are separated by 13 nucleotides PP_3190 and PP_3191 overlap by 4 nucleotides PP_3191 and PP_3192 are separated by 30 nucleotides PP_3192 and PP_3193 are separated by 225 nucleotides
PP_3189: PP_3189 - cytosine deaminase / isoguanine deaminase, at 3,618,150 to 3,619,391
_3189
PP_3190: PP_3190 - Ornithine cyclodeaminase/mu-crystallin family protein, at 3,619,405 to 3,620,352
_3190
PP_3191: PP_3191 - putative threonine ammonia-lyase / dehydratase, at 3,620,349 to 3,621,401
_3191
PP_3192: PP_3192 - conserved protein of unknown function, at 3,621,432 to 3,622,037
_3192
PP_3193: PP_3193 - conserved protein of unknown function, at 3,622,263 to 3,622,721
_3193
Position (kb)
3620
3621
3622 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3619.482 kb on + strand at 3619.482 kb on + strand at 3619.702 kb on - strand, within PP_3190 at 3619.847 kb on + strand, within PP_3190 at 3620.135 kb on + strand, within PP_3190 at 3620.549 kb on + strand, within PP_3191 at 3620.829 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.883 kb on + strand, within PP_3191 at 3621.013 kb on + strand, within PP_3191 at 3621.014 kb on - strand, within PP_3191 at 3621.014 kb on - strand, within PP_3191 at 3621.299 kb on + strand at 3621.300 kb on - strand at 3621.300 kb on - strand at 3621.300 kb on - strand at 3621.454 kb on + strand at 3621.455 kb on - strand at 3621.460 kb on + strand at 3621.500 kb on - strand, within PP_3192 at 3621.596 kb on + strand, within PP_3192 at 3621.760 kb on + strand, within PP_3192 at 3621.855 kb on - strand, within PP_3192 at 3621.923 kb on + strand, within PP_3192 at 3621.933 kb on - strand, within PP_3192 at 3621.955 kb on - strand, within PP_3192 at 3621.985 kb on + strand at 3622.043 kb on - strand at 3622.069 kb on - strand at 3622.069 kb on - strand at 3622.087 kb on - strand at 3622.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,619,482 + +1.0 3,619,482 + -0.6 3,619,702 - PP_3190 0.31 +0.4 3,619,847 + PP_3190 0.47 +0.6 3,620,135 + PP_3190 0.77 +0.2 3,620,549 + PP_3191 0.19 -0.6 3,620,829 + PP_3191 0.46 -1.2 3,620,866 + PP_3191 0.49 +2.1 3,620,866 + PP_3191 0.49 -0.8 3,620,866 + PP_3191 0.49 +0.8 3,620,883 + PP_3191 0.51 +0.4 3,621,013 + PP_3191 0.63 +1.0 3,621,014 - PP_3191 0.63 +1.0 3,621,014 - PP_3191 0.63 +0.2 3,621,299 + +0.2 3,621,300 - +0.2 3,621,300 - -1.3 3,621,300 - -0.7 3,621,454 + +1.5 3,621,455 - +0.7 3,621,460 + +0.6 3,621,500 - PP_3192 0.11 +0.4 3,621,596 + PP_3192 0.27 +0.3 3,621,760 + PP_3192 0.54 -0.3 3,621,855 - PP_3192 0.70 +0.0 3,621,923 + PP_3192 0.81 -1.5 3,621,933 - PP_3192 0.83 +1.0 3,621,955 - PP_3192 0.86 -2.0 3,621,985 + +0.6 3,622,043 - -0.4 3,622,069 - -0.3 3,622,069 - -2.7 3,622,087 - +0.4 3,622,157 - +1.0
Or see this region's nucleotide sequence