Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2881 and PP_2882 are separated by 28 nucleotides PP_2882 and PP_2883 overlap by 28 nucleotides PP_2883 and PP_2884 are separated by 2 nucleotides PP_2884 and PP_2885 are separated by 81 nucleotides
PP_2881: PP_2881 - Oxidoreductase, short chain dehydrogenase/reductase family, at 3,280,719 to 3,281,450
_2881
PP_2882: PP_2882 - putative 2-hydroxychromene-2-carboxylate isomerase, at 3,281,479 to 3,282,093
_2882
PP_2883: PP_2883 - conserved protein of unknown function, at 3,282,066 to 3,282,365
_2883
PP_2884: PP_2884 - XRE family transcriptional regulator, at 3,282,368 to 3,282,913
_2884
PP_2885: PP_2885 - putative Transporter, at 3,282,995 to 3,284,224
_2885
Position (kb)
3282
3283 Strain fitness (log2 ratio)
-2
-1
0
1 at 3281.373 kb on + strand, within PP_2881 at 3281.374 kb on - strand, within PP_2881 at 3281.374 kb on - strand, within PP_2881 at 3281.448 kb on + strand at 3281.448 kb on + strand at 3281.528 kb on - strand at 3281.662 kb on + strand, within PP_2882 at 3281.663 kb on - strand, within PP_2882 at 3281.663 kb on - strand, within PP_2882 at 3281.740 kb on - strand, within PP_2882 at 3281.835 kb on - strand, within PP_2882 at 3281.835 kb on - strand, within PP_2882 at 3281.949 kb on - strand, within PP_2882 at 3282.136 kb on + strand, within PP_2883 at 3282.213 kb on - strand, within PP_2883 at 3282.456 kb on + strand, within PP_2884 at 3282.456 kb on + strand, within PP_2884 at 3282.456 kb on + strand, within PP_2884 at 3282.457 kb on - strand, within PP_2884 at 3282.457 kb on - strand, within PP_2884 at 3282.457 kb on - strand, within PP_2884 at 3282.459 kb on + strand, within PP_2884 at 3282.459 kb on + strand, within PP_2884 at 3282.588 kb on + strand, within PP_2884 at 3282.588 kb on + strand, within PP_2884 at 3282.589 kb on - strand, within PP_2884 at 3282.692 kb on + strand, within PP_2884 at 3282.693 kb on - strand, within PP_2884 at 3282.696 kb on + strand, within PP_2884 at 3282.924 kb on + strand at 3282.940 kb on + strand at 3282.943 kb on + strand at 3282.943 kb on + strand at 3282.961 kb on - strand at 3283.086 kb on + strand at 3283.087 kb on - strand at 3283.087 kb on - strand at 3283.087 kb on - strand at 3283.087 kb on - strand at 3283.096 kb on + strand at 3283.245 kb on + strand, within PP_2885 at 3283.246 kb on - strand, within PP_2885 at 3283.361 kb on + strand, within PP_2885 at 3283.362 kb on - strand, within PP_2885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,281,373 + PP_2881 0.89 -0.2 3,281,374 - PP_2881 0.89 -1.1 3,281,374 - PP_2881 0.89 +1.7 3,281,448 + -0.6 3,281,448 + +0.9 3,281,528 - +0.0 3,281,662 + PP_2882 0.30 -0.5 3,281,663 - PP_2882 0.30 -1.0 3,281,663 - PP_2882 0.30 -1.1 3,281,740 - PP_2882 0.42 +0.7 3,281,835 - PP_2882 0.58 -2.0 3,281,835 - PP_2882 0.58 -0.7 3,281,949 - PP_2882 0.76 -0.5 3,282,136 + PP_2883 0.23 +1.3 3,282,213 - PP_2883 0.49 +0.3 3,282,456 + PP_2884 0.16 -0.4 3,282,456 + PP_2884 0.16 +0.1 3,282,456 + PP_2884 0.16 -0.3 3,282,457 - PP_2884 0.16 +0.9 3,282,457 - PP_2884 0.16 +0.2 3,282,457 - PP_2884 0.16 -2.3 3,282,459 + PP_2884 0.17 -1.0 3,282,459 + PP_2884 0.17 +0.3 3,282,588 + PP_2884 0.40 +0.2 3,282,588 + PP_2884 0.40 +0.8 3,282,589 - PP_2884 0.40 -1.8 3,282,692 + PP_2884 0.59 +1.4 3,282,693 - PP_2884 0.60 -1.5 3,282,696 + PP_2884 0.60 -0.9 3,282,924 + -1.5 3,282,940 + +0.3 3,282,943 + -0.5 3,282,943 + -0.7 3,282,961 - +0.5 3,283,086 + +0.4 3,283,087 - -2.3 3,283,087 - -0.5 3,283,087 - -0.2 3,283,087 - +0.1 3,283,096 + -0.8 3,283,245 + PP_2885 0.20 +0.5 3,283,246 - PP_2885 0.20 +0.6 3,283,361 + PP_2885 0.30 -0.1 3,283,362 - PP_2885 0.30 +0.6
Or see this region's nucleotide sequence