Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2856 and PP_2857 are separated by 49 nucleotides PP_2857 and PP_2858 are separated by 1 nucleotides PP_2858 and PP_2859 are separated by 297 nucleotides PP_2859 and PP_2860 overlap by 4 nucleotides
PP_2856: PP_2856 - conserved exported protein of unknown function, at 3,258,740 to 3,260,191
_2856
PP_2857: PP_2857 - conserved exported protein of unknown function, at 3,260,241 to 3,260,819
_2857
PP_2858: PP_2858 - conserved exported protein of unknown function, at 3,260,821 to 3,261,258
_2858
PP_2859: PP_2859 - conserved protein of unknown function, at 3,261,556 to 3,262,125
_2859
PP_2860: PP_2860 - putative Nicotinamide mononucleotide transporter, at 3,262,122 to 3,262,688
_2860
Position (kb)
3260
3261
3262 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3259.913 kb on + strand, within PP_2856 at 3259.913 kb on + strand, within PP_2856 at 3259.913 kb on + strand, within PP_2856 at 3260.034 kb on + strand, within PP_2856 at 3260.077 kb on + strand at 3260.078 kb on - strand at 3260.078 kb on - strand at 3260.101 kb on + strand at 3260.126 kb on + strand at 3260.127 kb on - strand at 3260.209 kb on + strand at 3260.210 kb on - strand at 3260.212 kb on + strand at 3260.231 kb on - strand at 3260.348 kb on + strand, within PP_2857 at 3260.710 kb on + strand, within PP_2857 at 3260.896 kb on - strand, within PP_2858 at 3261.096 kb on + strand, within PP_2858 at 3261.208 kb on + strand, within PP_2858 at 3261.209 kb on - strand, within PP_2858 at 3261.223 kb on + strand at 3261.223 kb on + strand at 3261.226 kb on + strand at 3261.226 kb on + strand at 3261.226 kb on + strand at 3261.226 kb on + strand at 3261.226 kb on + strand at 3261.226 kb on + strand at 3261.226 kb on + strand at 3261.227 kb on - strand at 3261.227 kb on - strand at 3261.227 kb on - strand at 3261.233 kb on + strand at 3261.233 kb on + strand at 3261.234 kb on - strand at 3261.354 kb on - strand at 3261.600 kb on + strand at 3261.600 kb on + strand at 3261.601 kb on - strand at 3261.601 kb on - strand at 3261.652 kb on + strand, within PP_2859 at 3261.652 kb on + strand, within PP_2859 at 3261.872 kb on - strand, within PP_2859 at 3261.872 kb on - strand, within PP_2859 at 3261.872 kb on - strand, within PP_2859 at 3261.892 kb on + strand, within PP_2859 at 3261.892 kb on + strand, within PP_2859 at 3261.968 kb on + strand, within PP_2859 at 3261.969 kb on - strand, within PP_2859 at 3262.011 kb on - strand, within PP_2859 at 3262.033 kb on + strand, within PP_2859 at 3262.094 kb on - strand at 3262.131 kb on + strand at 3262.132 kb on - strand at 3262.232 kb on - strand, within PP_2860 at 3262.244 kb on - strand, within PP_2860 at 3262.244 kb on - strand, within PP_2860 at 3262.244 kb on - strand, within PP_2860 at 3262.244 kb on - strand, within PP_2860 at 3262.244 kb on - strand at 3262.244 kb on - strand, within PP_2860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,259,913 + PP_2856 0.81 +1.5 3,259,913 + PP_2856 0.81 +0.7 3,259,913 + PP_2856 0.81 -1.1 3,260,034 + PP_2856 0.89 -1.4 3,260,077 + -0.7 3,260,078 - -0.4 3,260,078 - +1.5 3,260,101 + +0.0 3,260,126 + +0.2 3,260,127 - +1.0 3,260,209 + -0.6 3,260,210 - +0.8 3,260,212 + +1.1 3,260,231 - +0.5 3,260,348 + PP_2857 0.18 -0.8 3,260,710 + PP_2857 0.81 +0.6 3,260,896 - PP_2858 0.17 +0.3 3,261,096 + PP_2858 0.63 -1.7 3,261,208 + PP_2858 0.88 -0.7 3,261,209 - PP_2858 0.89 -2.1 3,261,223 + +1.6 3,261,223 + -0.8 3,261,226 + +0.4 3,261,226 + +0.1 3,261,226 + +0.5 3,261,226 + -0.9 3,261,226 + -0.2 3,261,226 + -0.7 3,261,226 + +1.4 3,261,227 - +0.3 3,261,227 - -0.1 3,261,227 - -0.8 3,261,233 + +0.1 3,261,233 + +1.1 3,261,234 - -0.6 3,261,354 - -0.5 3,261,600 + -0.8 3,261,600 + -0.6 3,261,601 - +0.2 3,261,601 - +0.6 3,261,652 + PP_2859 0.17 +0.6 3,261,652 + PP_2859 0.17 +0.4 3,261,872 - PP_2859 0.55 -1.3 3,261,872 - PP_2859 0.55 -3.8 3,261,872 - PP_2859 0.55 -0.4 3,261,892 + PP_2859 0.59 -1.3 3,261,892 + PP_2859 0.59 +0.4 3,261,968 + PP_2859 0.72 -3.5 3,261,969 - PP_2859 0.72 -0.5 3,262,011 - PP_2859 0.80 -0.2 3,262,033 + PP_2859 0.84 +0.9 3,262,094 - -0.2 3,262,131 + -0.7 3,262,132 - -0.6 3,262,232 - PP_2860 0.19 -0.5 3,262,244 - PP_2860 0.22 +1.0 3,262,244 - PP_2860 0.22 -1.1 3,262,244 - PP_2860 0.22 +0.4 3,262,244 - PP_2860 0.22 -0.2 3,262,244 - -0.5 3,262,244 - PP_2860 0.22 +0.1
Or see this region's nucleotide sequence