Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2748 and PP_2749 overlap by 4 nucleotides PP_2749 and PP_2750 are separated by 4 nucleotides PP_2750 and PP_2751 are separated by 78 nucleotides
PP_2748: PP_2748 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,131,122 to 3,131,922
_2748
PP_2749: PP_2749 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,131,919 to 3,132,800
_2749
PP_2750: PP_2750 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,132,805 to 3,133,869
_2750
PP_2751: PP_2751 - conserved exported protein of unknown function, at 3,133,948 to 3,135,288
_2751
Position (kb)
3132
3133
3134 Strain fitness (log2 ratio)
-1
0
1
2 at 3131.841 kb on + strand, within PP_2748 at 3131.841 kb on + strand, within PP_2748 at 3132.003 kb on + strand at 3132.003 kb on + strand at 3132.122 kb on - strand, within PP_2749 at 3132.269 kb on - strand, within PP_2749 at 3132.574 kb on + strand, within PP_2749 at 3132.574 kb on + strand, within PP_2749 at 3132.682 kb on - strand, within PP_2749 at 3132.751 kb on - strand at 3132.751 kb on - strand at 3132.751 kb on - strand at 3132.751 kb on - strand at 3132.797 kb on - strand at 3133.265 kb on - strand, within PP_2750 at 3133.484 kb on - strand, within PP_2750 at 3133.484 kb on - strand, within PP_2750 at 3133.517 kb on - strand, within PP_2750 at 3133.815 kb on - strand at 3133.815 kb on - strand at 3133.815 kb on - strand at 3133.842 kb on + strand at 3134.066 kb on - strand at 3134.080 kb on + strand at 3134.131 kb on + strand, within PP_2751 at 3134.234 kb on - strand at 3134.443 kb on + strand, within PP_2751 at 3134.444 kb on - strand, within PP_2751 at 3134.444 kb on - strand, within PP_2751 at 3134.444 kb on - strand, within PP_2751 at 3134.518 kb on + strand, within PP_2751 at 3134.563 kb on + strand, within PP_2751 at 3134.564 kb on - strand, within PP_2751 at 3134.697 kb on - strand, within PP_2751 at 3134.867 kb on + strand at 3134.867 kb on + strand, within PP_2751 at 3134.868 kb on - strand, within PP_2751 at 3134.868 kb on - strand, within PP_2751 at 3134.868 kb on - strand, within PP_2751
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,131,841 + PP_2748 0.90 -0.7 3,131,841 + PP_2748 0.90 -0.3 3,132,003 + -1.0 3,132,003 + -0.3 3,132,122 - PP_2749 0.23 +0.2 3,132,269 - PP_2749 0.40 -0.2 3,132,574 + PP_2749 0.74 -0.5 3,132,574 + PP_2749 0.74 -0.4 3,132,682 - PP_2749 0.87 +1.8 3,132,751 - +0.1 3,132,751 - +0.6 3,132,751 - -0.1 3,132,751 - -1.3 3,132,797 - +0.6 3,133,265 - PP_2750 0.43 +0.1 3,133,484 - PP_2750 0.64 -0.7 3,133,484 - PP_2750 0.64 +1.8 3,133,517 - PP_2750 0.67 +1.2 3,133,815 - +0.5 3,133,815 - +0.5 3,133,815 - +0.0 3,133,842 + +0.9 3,134,066 - +0.2 3,134,080 + +1.1 3,134,131 + PP_2751 0.14 -0.4 3,134,234 - +0.8 3,134,443 + PP_2751 0.37 -0.4 3,134,444 - PP_2751 0.37 +1.0 3,134,444 - PP_2751 0.37 -0.9 3,134,444 - PP_2751 0.37 +1.7 3,134,518 + PP_2751 0.43 -0.3 3,134,563 + PP_2751 0.46 -0.5 3,134,564 - PP_2751 0.46 -1.0 3,134,697 - PP_2751 0.56 -0.7 3,134,867 + -0.9 3,134,867 + PP_2751 0.69 -1.6 3,134,868 - PP_2751 0.69 -0.3 3,134,868 - PP_2751 0.69 +0.3 3,134,868 - PP_2751 0.69 -0.2
Or see this region's nucleotide sequence