Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0274 and PP_0275 are separated by 419 nucleotides PP_0275 and PP_0276 overlap by 4 nucleotides PP_0276 and PP_t73 are separated by 287 nucleotides PP_t73 and PP_0277 are separated by 98 nucleotides PP_0277 and PP_5437 are separated by 203 nucleotides
PP_0274: PP_0274 - putative transcriptional regulator containing HTH domain, at 333,257 to 333,730
_0274
PP_0275: PP_0275 - conserved protein of unknown function, at 334,150 to 334,365
_0275
PP_0276: PP_0276 - Transcriptional regulator, Cro/CI family, at 334,362 to 334,685
_0276
PP_t73: PP_t73 - tRNA-Thr, at 334,973 to 335,048
_t73
PP_0277: PP_0277 - conserved exported protein of unknown function, at 335,147 to 335,395
_0277
PP_5437: PP_5437 - protein of unknown function, at 335,599 to 335,781
_5437
Position (kb) 334
335 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 333.522 kb on + strand, within PP_0274 at 333.522 kb on + strand, within PP_0274 at 333.830 kb on - strand at 333.864 kb on - strand at 333.940 kb on + strand at 333.940 kb on + strand at 333.940 kb on + strand at 334.004 kb on + strand at 334.020 kb on + strand at 334.085 kb on - strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.117 kb on + strand at 334.117 kb on + strand at 334.118 kb on - strand at 334.118 kb on - strand at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.363 kb on + strand at 334.364 kb on - strand at 334.364 kb on - strand at 334.364 kb on - strand at 334.531 kb on - strand, within PP_0276 at 334.866 kb on + strand at 334.897 kb on + strand at 334.965 kb on - strand at 334.971 kb on + strand at 334.972 kb on - strand at 334.972 kb on - strand at 335.065 kb on - strand at 335.119 kb on - strand at 335.505 kb on + strand at 335.505 kb on + strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.548 kb on - strand at 335.548 kb on - strand at 335.548 kb on - strand at 335.577 kb on - strand at 335.600 kb on + strand at 335.601 kb on - strand at 335.608 kb on + strand at 335.663 kb on + strand, within PP_5437 at 335.664 kb on - strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.669 kb on - strand, within PP_5437 at 335.669 kb on - strand, within PP_5437
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 333,522 + PP_0274 0.56 +0.3 333,522 + PP_0274 0.56 +0.5 333,830 - -0.3 333,864 - +0.5 333,940 + +0.1 333,940 + -0.0 333,940 + -0.8 334,004 + +0.2 334,020 + +0.4 334,085 - +0.7 334,112 + +0.8 334,112 + -0.6 334,112 + -2.2 334,112 + +1.2 334,112 + +0.9 334,112 + +0.1 334,112 + +0.1 334,113 - +0.6 334,113 - +1.5 334,113 - -0.1 334,113 - -0.9 334,117 + +0.2 334,117 + +0.1 334,118 - -0.1 334,118 - -0.8 334,219 + PP_0275 0.32 +1.0 334,219 + PP_0275 0.32 -2.8 334,219 + PP_0275 0.32 -1.1 334,219 + PP_0275 0.32 +0.0 334,219 + PP_0275 0.32 -0.3 334,220 - PP_0275 0.32 -0.7 334,220 - PP_0275 0.32 -1.1 334,220 - PP_0275 0.32 -0.7 334,220 - PP_0275 0.32 +0.6 334,363 + +0.3 334,364 - +0.1 334,364 - +0.8 334,364 - +0.0 334,531 - PP_0276 0.52 +1.0 334,866 + -0.3 334,897 + +0.2 334,965 - -0.9 334,971 + -0.2 334,972 - +0.3 334,972 - +0.2 335,065 - +0.2 335,119 - +0.8 335,505 + +0.2 335,505 + -0.8 335,506 - +0.2 335,506 - -1.1 335,506 - -0.3 335,506 - -0.0 335,506 - -0.8 335,548 - -0.1 335,548 - -0.2 335,548 - -0.2 335,577 - +0.0 335,600 + +1.6 335,601 - -1.1 335,608 + -1.5 335,663 + PP_5437 0.35 +1.4 335,664 - PP_5437 0.36 +0.0 335,668 + PP_5437 0.38 +0.0 335,668 + PP_5437 0.38 +1.3 335,668 + PP_5437 0.38 +1.2 335,669 - PP_5437 0.38 +0.2 335,669 - PP_5437 0.38 +0.4
Or see this region's nucleotide sequence