Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4238 and PP_5665 are separated by 392 nucleotides PP_5665 and PP_5666 are separated by 58 nucleotides PP_5666 and PP_5667 overlap by 1 nucleotides PP_5667 and PP_4240 are separated by 4 nucleotides
PP_4238: PP_4238 - conserved protein of unknown function, at 4,811,757 to 4,812,080
_4238
PP_5665: PP_5665 - putative microcin B17-like protein, McbA family, at 4,812,473 to 4,812,715
_5665
PP_5666: PP_5666 - putative microcin B17-like processing protein, McbB family, at 4,812,774 to 4,813,637
_5666
PP_5667: PP_5667 - putative microcin B17-like-processing protein, McbC family, at 4,813,637 to 4,814,428
_5667
PP_4240: PP_4240 - putative Microcin b17 processing protein mcbd, at 4,814,433 to 4,815,635
_4240
Position (kb)
4812
4813
4814 Strain fitness (log2 ratio)
-1
0
1 at 4811.995 kb on + strand, within PP_4238 at 4812.017 kb on + strand, within PP_4238 at 4812.017 kb on + strand, within PP_4238 at 4812.078 kb on + strand at 4812.131 kb on + strand at 4812.150 kb on - strand at 4812.230 kb on + strand at 4812.452 kb on + strand at 4812.453 kb on - strand at 4812.523 kb on + strand, within PP_5665 at 4812.692 kb on - strand at 4813.050 kb on + strand, within PP_5666 at 4813.149 kb on - strand, within PP_5666 at 4813.168 kb on + strand, within PP_5666 at 4813.229 kb on - strand, within PP_5666 at 4813.353 kb on - strand, within PP_5666 at 4813.475 kb on - strand, within PP_5666 at 4813.632 kb on - strand at 4813.721 kb on + strand, within PP_5667 at 4813.721 kb on + strand, within PP_5667 at 4813.962 kb on + strand, within PP_5667 at 4814.203 kb on + strand, within PP_5667 at 4814.207 kb on + strand, within PP_5667 at 4814.241 kb on - strand at 4814.288 kb on + strand, within PP_5667 at 4814.349 kb on - strand at 4814.350 kb on + strand at 4814.486 kb on + strand at 4814.547 kb on + strand at 4814.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 4,811,995 + PP_4238 0.73 +0.8 4,812,017 + PP_4238 0.80 -0.7 4,812,017 + PP_4238 0.80 -0.2 4,812,078 + -0.4 4,812,131 + +0.2 4,812,150 - -0.1 4,812,230 + +0.5 4,812,452 + -0.0 4,812,453 - -0.4 4,812,523 + PP_5665 0.21 +0.7 4,812,692 - +0.0 4,813,050 + PP_5666 0.32 -0.1 4,813,149 - PP_5666 0.43 -0.7 4,813,168 + PP_5666 0.46 -0.6 4,813,229 - PP_5666 0.53 -0.9 4,813,353 - PP_5666 0.67 +0.5 4,813,475 - PP_5666 0.81 -1.3 4,813,632 - -0.8 4,813,721 + PP_5667 0.11 -0.3 4,813,721 + PP_5667 0.11 +0.3 4,813,962 + PP_5667 0.41 -0.3 4,814,203 + PP_5667 0.71 -1.5 4,814,207 + PP_5667 0.72 -0.4 4,814,241 - -0.0 4,814,288 + PP_5667 0.82 -0.2 4,814,349 - +0.4 4,814,350 + -0.5 4,814,486 + -1.6 4,814,547 + -0.3 4,814,547 + +0.3
Or see this region's nucleotide sequence