Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3503 and PP_3504 are separated by 340 nucleotides PP_3504 and PP_3505 are separated by 106 nucleotides PP_3505 and PP_3506 are separated by 77 nucleotides
PP_3503: PP_3503 - Sigma-54 dependent transcriptional regulator, at 3,973,147 to 3,974,472
_3503
PP_3504: PP_3504 - conserved protein of unknown function, at 3,974,813 to 3,975,079
_3504
PP_3505: PP_3505 - conserved protein of unknown function, at 3,975,186 to 3,975,776
_3505
PP_3506: PP_3506 - Magnesium chelatase, subunit ChII, at 3,975,854 to 3,976,867
_3506
Position (kb)
3974
3975
3976 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3974.137 kb on + strand, within PP_3503 at 3974.137 kb on + strand, within PP_3503 at 3974.138 kb on - strand, within PP_3503 at 3974.324 kb on - strand, within PP_3503 at 3974.364 kb on + strand at 3974.364 kb on + strand at 3974.365 kb on - strand at 3974.365 kb on - strand at 3974.405 kb on - strand at 3974.541 kb on + strand at 3974.541 kb on + strand at 3974.694 kb on + strand at 3974.694 kb on + strand at 3974.717 kb on + strand at 3974.717 kb on + strand at 3974.718 kb on - strand at 3974.723 kb on + strand at 3974.799 kb on - strand at 3974.799 kb on - strand at 3974.884 kb on - strand, within PP_3504 at 3974.905 kb on + strand, within PP_3504 at 3974.905 kb on + strand, within PP_3504 at 3974.981 kb on + strand, within PP_3504 at 3974.981 kb on + strand, within PP_3504 at 3974.981 kb on + strand, within PP_3504 at 3974.982 kb on - strand, within PP_3504 at 3974.982 kb on - strand, within PP_3504 at 3974.982 kb on - strand, within PP_3504 at 3975.077 kb on + strand at 3975.078 kb on - strand at 3975.078 kb on - strand at 3975.121 kb on + strand at 3975.121 kb on + strand at 3975.121 kb on + strand at 3975.220 kb on + strand at 3975.221 kb on - strand at 3975.221 kb on - strand at 3975.324 kb on - strand, within PP_3505 at 3975.403 kb on - strand, within PP_3505 at 3975.568 kb on + strand, within PP_3505 at 3976.021 kb on + strand, within PP_3506 at 3976.078 kb on + strand, within PP_3506 at 3976.078 kb on + strand, within PP_3506
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,974,137 + PP_3503 0.75 +0.1 3,974,137 + PP_3503 0.75 +0.4 3,974,138 - PP_3503 0.75 +1.1 3,974,324 - PP_3503 0.89 -0.2 3,974,364 + +1.3 3,974,364 + +0.4 3,974,365 - +0.6 3,974,365 - +0.4 3,974,405 - +0.3 3,974,541 + +0.3 3,974,541 + -0.2 3,974,694 + -0.5 3,974,694 + +0.1 3,974,717 + -0.4 3,974,717 + -0.9 3,974,718 - -0.8 3,974,723 + -0.4 3,974,799 - -0.4 3,974,799 - -0.6 3,974,884 - PP_3504 0.27 -0.3 3,974,905 + PP_3504 0.34 -0.2 3,974,905 + PP_3504 0.34 -1.5 3,974,981 + PP_3504 0.63 -0.6 3,974,981 + PP_3504 0.63 -1.1 3,974,981 + PP_3504 0.63 -0.6 3,974,982 - PP_3504 0.63 -0.4 3,974,982 - PP_3504 0.63 -0.4 3,974,982 - PP_3504 0.63 -0.8 3,975,077 + +0.2 3,975,078 - -1.0 3,975,078 - -3.2 3,975,121 + -0.8 3,975,121 + -0.4 3,975,121 + +1.2 3,975,220 + -0.3 3,975,221 - -0.4 3,975,221 - -0.8 3,975,324 - PP_3505 0.23 +0.1 3,975,403 - PP_3505 0.37 -0.7 3,975,568 + PP_3505 0.65 -0.5 3,976,021 + PP_3506 0.16 -1.4 3,976,078 + PP_3506 0.22 +1.9 3,976,078 + PP_3506 0.22 -0.3
Or see this region's nucleotide sequence