Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0483 and PP_0484 are separated by 129 nucleotides PP_0484 and PP_0485 are separated by 9 nucleotides PP_0485 and PP_0486 are separated by 90 nucleotides
PP_0483: PP_0483 - excinuclease (uvrABC system protein A), at 566,661 to 569,495
_0483
PP_0484: PP_0484 - transporter of an unknown substrate, at 569,625 to 571,019
_0484
PP_0485: PP_0485 - Single-stranded DNA-binding protein, at 571,029 to 571,574
_0485
PP_0486: PP_0486 - Transcriptional regulator, GntR family, at 571,665 to 573,089
_0486
Position (kb) 569
570
571
572 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 569.518 kb on - strand at 569.529 kb on + strand at 569.530 kb on - strand at 569.530 kb on - strand at 569.567 kb on - strand at 569.705 kb on + strand at 569.706 kb on - strand at 569.734 kb on + strand at 569.745 kb on + strand at 569.802 kb on + strand, within PP_0484 at 569.802 kb on + strand, within PP_0484 at 569.803 kb on - strand, within PP_0484 at 569.803 kb on - strand, within PP_0484 at 569.878 kb on - strand, within PP_0484 at 569.911 kb on + strand, within PP_0484 at 569.911 kb on + strand, within PP_0484 at 569.912 kb on - strand, within PP_0484 at 569.912 kb on - strand, within PP_0484 at 569.933 kb on + strand, within PP_0484 at 570.393 kb on + strand, within PP_0484 at 570.394 kb on - strand at 570.394 kb on - strand, within PP_0484 at 570.444 kb on + strand, within PP_0484 at 570.444 kb on + strand, within PP_0484 at 570.444 kb on + strand, within PP_0484 at 570.445 kb on - strand, within PP_0484 at 570.445 kb on - strand, within PP_0484 at 570.445 kb on - strand, within PP_0484 at 570.514 kb on + strand, within PP_0484 at 570.515 kb on - strand, within PP_0484 at 570.516 kb on + strand, within PP_0484 at 570.516 kb on + strand, within PP_0484 at 570.516 kb on + strand, within PP_0484 at 570.663 kb on + strand, within PP_0484 at 570.773 kb on + strand, within PP_0484 at 570.813 kb on + strand, within PP_0484 at 570.813 kb on + strand, within PP_0484 at 570.813 kb on + strand, within PP_0484 at 570.936 kb on + strand at 570.936 kb on + strand at 570.938 kb on + strand at 571.669 kb on + strand at 571.890 kb on - strand, within PP_0486 at 571.890 kb on - strand, within PP_0486 at 571.890 kb on - strand, within PP_0486 at 571.921 kb on + strand, within PP_0486 at 571.922 kb on - strand, within PP_0486 at 571.979 kb on + strand, within PP_0486
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 569,518 - +0.7 569,529 + -2.3 569,530 - +0.1 569,530 - -2.3 569,567 - +0.6 569,705 + +0.3 569,706 - -2.2 569,734 + +2.1 569,745 + +2.4 569,802 + PP_0484 0.13 -0.2 569,802 + PP_0484 0.13 +0.5 569,803 - PP_0484 0.13 -0.3 569,803 - PP_0484 0.13 -1.6 569,878 - PP_0484 0.18 +3.8 569,911 + PP_0484 0.21 -1.0 569,911 + PP_0484 0.21 +1.2 569,912 - PP_0484 0.21 -0.7 569,912 - PP_0484 0.21 -2.5 569,933 + PP_0484 0.22 -1.2 570,393 + PP_0484 0.55 -1.4 570,394 - +0.4 570,394 - PP_0484 0.55 -0.8 570,444 + PP_0484 0.59 +0.7 570,444 + PP_0484 0.59 -0.1 570,444 + PP_0484 0.59 -0.8 570,445 - PP_0484 0.59 -2.0 570,445 - PP_0484 0.59 -1.3 570,445 - PP_0484 0.59 -0.8 570,514 + PP_0484 0.64 -2.3 570,515 - PP_0484 0.64 -1.8 570,516 + PP_0484 0.64 -1.1 570,516 + PP_0484 0.64 -1.0 570,516 + PP_0484 0.64 -0.5 570,663 + PP_0484 0.74 -1.8 570,773 + PP_0484 0.82 -2.0 570,813 + PP_0484 0.85 +1.0 570,813 + PP_0484 0.85 -0.3 570,813 + PP_0484 0.85 -0.6 570,936 + +0.1 570,936 + +0.3 570,938 + +0.5 571,669 + -0.3 571,890 - PP_0486 0.16 -0.1 571,890 - PP_0486 0.16 +1.3 571,890 - PP_0486 0.16 +0.1 571,921 + PP_0486 0.18 -0.3 571,922 - PP_0486 0.18 -0.8 571,979 + PP_0486 0.22 +0.5
Or see this region's nucleotide sequence