Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4818 and PP_4819 are separated by 42 nucleotides PP_4819 and PP_4820 are separated by 207 nucleotides
PP_4818: PP_4818 - ribosomal protein L11 methyltransferase, at 5,479,477 to 5,480,469
_4818
PP_4819: PP_4819 - conserved protein of unknown function, at 5,480,512 to 5,481,867
_4819
PP_4820: PP_4820 - tRNA-dihydrouridine synthase B, at 5,482,075 to 5,483,088
_4820
Position (kb)
5480
5481
5482 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5479.574 kb on + strand at 5479.575 kb on - strand at 5479.575 kb on - strand at 5479.607 kb on + strand, within PP_4818 at 5479.642 kb on + strand, within PP_4818 at 5479.642 kb on + strand, within PP_4818 at 5479.643 kb on - strand, within PP_4818 at 5479.646 kb on + strand, within PP_4818 at 5479.664 kb on + strand, within PP_4818 at 5480.142 kb on - strand, within PP_4818 at 5480.227 kb on + strand, within PP_4818 at 5480.228 kb on - strand, within PP_4818 at 5480.396 kb on - strand at 5480.477 kb on + strand at 5480.478 kb on - strand at 5480.478 kb on - strand at 5480.483 kb on - strand at 5480.624 kb on + strand at 5480.625 kb on - strand at 5480.756 kb on + strand, within PP_4819 at 5480.968 kb on + strand, within PP_4819 at 5480.968 kb on + strand, within PP_4819 at 5480.969 kb on - strand, within PP_4819 at 5480.969 kb on - strand, within PP_4819 at 5480.969 kb on - strand, within PP_4819 at 5481.257 kb on + strand, within PP_4819 at 5481.363 kb on - strand, within PP_4819 at 5481.452 kb on - strand, within PP_4819 at 5481.494 kb on - strand, within PP_4819 at 5481.556 kb on - strand, within PP_4819 at 5481.587 kb on + strand, within PP_4819 at 5481.637 kb on + strand, within PP_4819 at 5481.637 kb on + strand, within PP_4819 at 5481.739 kb on + strand at 5481.739 kb on + strand at 5481.739 kb on + strand at 5481.865 kb on + strand at 5481.959 kb on + strand at 5481.959 kb on + strand at 5481.960 kb on - strand at 5481.960 kb on - strand at 5482.074 kb on + strand at 5482.074 kb on + strand at 5482.074 kb on + strand at 5482.106 kb on + strand at 5482.106 kb on + strand at 5482.106 kb on + strand at 5482.106 kb on + strand at 5482.106 kb on + strand at 5482.110 kb on + strand at 5482.110 kb on + strand at 5482.110 kb on + strand at 5482.110 kb on + strand at 5482.188 kb on + strand, within PP_4820 at 5482.368 kb on + strand, within PP_4820 at 5482.424 kb on + strand, within PP_4820 at 5482.630 kb on + strand, within PP_4820 at 5482.801 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,479,574 + -0.2 5,479,575 - -2.0 5,479,575 - +0.9 5,479,607 + PP_4818 0.13 +0.2 5,479,642 + PP_4818 0.17 -0.3 5,479,642 + PP_4818 0.17 -0.1 5,479,643 - PP_4818 0.17 -1.1 5,479,646 + PP_4818 0.17 -0.7 5,479,664 + PP_4818 0.19 -0.9 5,480,142 - PP_4818 0.67 +0.8 5,480,227 + PP_4818 0.76 +0.6 5,480,228 - PP_4818 0.76 +0.8 5,480,396 - -1.2 5,480,477 + -1.4 5,480,478 - -0.8 5,480,478 - -0.6 5,480,483 - -1.1 5,480,624 + +0.4 5,480,625 - +0.0 5,480,756 + PP_4819 0.18 -0.8 5,480,968 + PP_4819 0.34 -0.3 5,480,968 + PP_4819 0.34 -0.6 5,480,969 - PP_4819 0.34 -2.8 5,480,969 - PP_4819 0.34 +0.3 5,480,969 - PP_4819 0.34 +0.3 5,481,257 + PP_4819 0.55 -2.7 5,481,363 - PP_4819 0.63 -1.8 5,481,452 - PP_4819 0.69 -1.9 5,481,494 - PP_4819 0.72 +0.3 5,481,556 - PP_4819 0.77 -0.5 5,481,587 + PP_4819 0.79 -0.2 5,481,637 + PP_4819 0.83 -1.6 5,481,637 + PP_4819 0.83 -1.0 5,481,739 + -0.4 5,481,739 + -0.3 5,481,739 + -0.2 5,481,865 + -1.0 5,481,959 + -1.6 5,481,959 + +0.4 5,481,960 - -1.9 5,481,960 - +0.6 5,482,074 + -0.3 5,482,074 + -0.1 5,482,074 + -0.0 5,482,106 + +0.5 5,482,106 + -1.5 5,482,106 + +0.1 5,482,106 + -0.0 5,482,106 + -0.5 5,482,110 + -0.7 5,482,110 + +0.8 5,482,110 + -0.8 5,482,110 + -0.4 5,482,188 + PP_4820 0.11 -0.2 5,482,368 + PP_4820 0.29 +0.8 5,482,424 + PP_4820 0.34 -0.2 5,482,630 + PP_4820 0.55 -0.0 5,482,801 + -0.9
Or see this region's nucleotide sequence