Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4767 and PP_4768 are separated by 139 nucleotides PP_4768 and PP_4769 are separated by 9 nucleotides PP_4769 and PP_4770 are separated by 160 nucleotides
PP_4767: PP_4767 - amino acid exporter for phenylalanine, threonine, at 5,428,158 to 5,429,078
_4767
PP_4768: PP_4768 - Exonuclease, at 5,429,218 to 5,429,829
_4768
PP_4769: PP_4769 - conserved protein of unknown function, at 5,429,839 to 5,430,318
_4769
PP_4770: PP_4770 - conserved protein of unknown function, at 5,430,479 to 5,431,237
_4770
Position (kb)
5429
5430
5431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5428.884 kb on - strand, within PP_4767 at 5428.884 kb on - strand, within PP_4767 at 5428.918 kb on + strand, within PP_4767 at 5428.919 kb on - strand, within PP_4767 at 5429.002 kb on + strand at 5429.003 kb on - strand at 5429.003 kb on - strand at 5429.003 kb on - strand at 5429.006 kb on + strand at 5429.006 kb on + strand at 5429.006 kb on + strand at 5429.006 kb on + strand at 5429.006 kb on + strand at 5429.006 kb on + strand at 5429.006 kb on + strand at 5429.007 kb on - strand at 5429.007 kb on - strand at 5429.023 kb on + strand at 5429.024 kb on - strand at 5429.024 kb on - strand at 5429.117 kb on - strand at 5429.144 kb on - strand at 5429.231 kb on + strand at 5429.232 kb on - strand at 5429.330 kb on - strand, within PP_4768 at 5429.330 kb on - strand, within PP_4768 at 5429.330 kb on - strand, within PP_4768 at 5429.342 kb on + strand, within PP_4768 at 5429.342 kb on + strand, within PP_4768 at 5429.531 kb on + strand, within PP_4768 at 5429.532 kb on - strand, within PP_4768 at 5429.790 kb on + strand at 5429.791 kb on - strand at 5429.820 kb on + strand at 5429.820 kb on + strand at 5429.820 kb on + strand at 5429.821 kb on - strand at 5429.821 kb on - strand at 5429.840 kb on + strand at 5429.876 kb on + strand at 5429.877 kb on - strand at 5429.927 kb on + strand, within PP_4769 at 5429.927 kb on + strand at 5429.928 kb on - strand, within PP_4769 at 5429.928 kb on - strand, within PP_4769 at 5430.048 kb on + strand, within PP_4769 at 5430.182 kb on + strand, within PP_4769 at 5430.240 kb on - strand, within PP_4769 at 5430.275 kb on + strand at 5430.275 kb on + strand at 5430.276 kb on - strand at 5430.276 kb on - strand at 5430.278 kb on + strand at 5430.278 kb on + strand at 5430.279 kb on - strand at 5430.279 kb on - strand at 5430.279 kb on - strand at 5430.279 kb on - strand at 5430.466 kb on + strand at 5430.466 kb on + strand at 5430.486 kb on + strand at 5430.487 kb on - strand at 5430.487 kb on - strand at 5430.544 kb on - strand at 5430.616 kb on + strand, within PP_4770 at 5430.617 kb on - strand, within PP_4770 at 5430.869 kb on + strand, within PP_4770 at 5430.870 kb on - strand, within PP_4770 at 5430.870 kb on - strand, within PP_4770 at 5431.098 kb on + strand, within PP_4770 at 5431.098 kb on + strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.101 kb on + strand, within PP_4770 at 5431.102 kb on - strand, within PP_4770 at 5431.102 kb on - strand, within PP_4770 at 5431.289 kb on + strand at 5431.289 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,428,884 - PP_4767 0.79 +0.1 5,428,884 - PP_4767 0.79 -0.1 5,428,918 + PP_4767 0.83 -0.4 5,428,919 - PP_4767 0.83 -0.8 5,429,002 + -0.2 5,429,003 - -1.0 5,429,003 - -0.2 5,429,003 - +0.4 5,429,006 + -0.0 5,429,006 + +0.6 5,429,006 + +0.2 5,429,006 + +0.2 5,429,006 + +0.8 5,429,006 + -1.3 5,429,006 + -0.2 5,429,007 - +0.4 5,429,007 - +0.7 5,429,023 + -1.4 5,429,024 - -0.1 5,429,024 - -0.2 5,429,117 - -0.6 5,429,144 - -1.8 5,429,231 + +0.4 5,429,232 - -0.2 5,429,330 - PP_4768 0.18 -1.0 5,429,330 - PP_4768 0.18 -0.7 5,429,330 - PP_4768 0.18 +1.7 5,429,342 + PP_4768 0.20 -4.6 5,429,342 + PP_4768 0.20 -1.1 5,429,531 + PP_4768 0.51 +0.6 5,429,532 - PP_4768 0.51 -1.4 5,429,790 + -0.2 5,429,791 - -0.7 5,429,820 + -0.5 5,429,820 + -0.6 5,429,820 + +0.9 5,429,821 - -0.0 5,429,821 - +0.4 5,429,840 + +0.1 5,429,876 + +0.2 5,429,877 - -1.1 5,429,927 + PP_4769 0.18 -1.7 5,429,927 + +1.1 5,429,928 - PP_4769 0.19 -1.1 5,429,928 - PP_4769 0.19 -0.9 5,430,048 + PP_4769 0.44 -0.4 5,430,182 + PP_4769 0.71 +0.3 5,430,240 - PP_4769 0.84 -0.3 5,430,275 + -0.4 5,430,275 + +1.3 5,430,276 - -0.0 5,430,276 - +0.1 5,430,278 + -0.3 5,430,278 + -0.1 5,430,279 - -0.3 5,430,279 - +0.1 5,430,279 - -0.5 5,430,279 - +0.0 5,430,466 + -0.0 5,430,466 + +0.5 5,430,486 + +0.7 5,430,487 - +1.0 5,430,487 - -0.1 5,430,544 - +0.4 5,430,616 + PP_4770 0.18 +0.7 5,430,617 - PP_4770 0.18 +1.2 5,430,869 + PP_4770 0.51 +0.6 5,430,870 - PP_4770 0.52 -1.8 5,430,870 - PP_4770 0.52 +0.8 5,431,098 + PP_4770 0.82 +0.4 5,431,098 + PP_4770 0.82 -0.6 5,431,099 - PP_4770 0.82 +0.7 5,431,099 - PP_4770 0.82 -0.2 5,431,099 - PP_4770 0.82 -0.7 5,431,099 - PP_4770 0.82 +1.4 5,431,099 - PP_4770 0.82 +0.5 5,431,099 - PP_4770 0.82 -0.3 5,431,099 - PP_4770 0.82 +0.3 5,431,101 + PP_4770 0.82 +0.6 5,431,102 - PP_4770 0.82 -2.0 5,431,102 - PP_4770 0.82 +0.4 5,431,289 + -0.1 5,431,289 + -1.5
Or see this region's nucleotide sequence