Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3256 and PP_3257 overlap by 4 nucleotides PP_3257 and PP_3258 are separated by 11 nucleotides PP_3258 and PP_3259 overlap by 4 nucleotides
PP_3256: PP_3256 - Glycosyl transferase, group 2 family protein, at 3,690,563 to 3,691,222
_3256
PP_3257: PP_3257 - putative Methyltransferase, at 3,691,219 to 3,691,818
_3257
PP_3258: PP_3258 - conserved protein of unknown function, at 3,691,830 to 3,692,585
_3258
PP_3259: PP_3259 - Acyl-CoA dehydrogenase-related protein, at 3,692,582 to 3,693,625
_3259
Position (kb)
3691
3692
3693 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3690.902 kb on - strand, within PP_3256 at 3691.050 kb on - strand, within PP_3256 at 3691.100 kb on + strand, within PP_3256 at 3691.196 kb on - strand at 3691.269 kb on - strand at 3691.269 kb on - strand at 3691.334 kb on - strand, within PP_3257 at 3691.413 kb on - strand, within PP_3257 at 3691.469 kb on + strand, within PP_3257 at 3691.469 kb on + strand, within PP_3257 at 3691.469 kb on + strand, within PP_3257 at 3691.470 kb on - strand, within PP_3257 at 3691.470 kb on - strand, within PP_3257 at 3691.470 kb on - strand, within PP_3257 at 3691.470 kb on - strand, within PP_3257 at 3691.470 kb on - strand, within PP_3257 at 3691.470 kb on - strand, within PP_3257 at 3691.472 kb on + strand, within PP_3257 at 3691.473 kb on - strand, within PP_3257 at 3691.473 kb on - strand, within PP_3257 at 3691.691 kb on + strand, within PP_3257 at 3691.692 kb on - strand, within PP_3257 at 3691.692 kb on - strand, within PP_3257 at 3691.742 kb on + strand, within PP_3257 at 3691.784 kb on + strand at 3691.786 kb on + strand at 3691.786 kb on + strand at 3691.787 kb on - strand at 3691.787 kb on - strand at 3691.787 kb on - strand at 3691.787 kb on - strand at 3691.800 kb on - strand at 3692.046 kb on + strand, within PP_3258 at 3692.461 kb on - strand, within PP_3258 at 3692.461 kb on - strand, within PP_3258 at 3692.585 kb on + strand at 3692.585 kb on + strand at 3692.585 kb on + strand at 3692.586 kb on - strand at 3692.586 kb on - strand at 3692.586 kb on - strand at 3692.586 kb on - strand at 3692.586 kb on - strand at 3692.750 kb on + strand, within PP_3259 at 3692.750 kb on + strand, within PP_3259 at 3692.750 kb on + strand, within PP_3259 at 3692.751 kb on - strand, within PP_3259 at 3692.774 kb on + strand, within PP_3259 at 3693.031 kb on - strand, within PP_3259 at 3693.065 kb on - strand, within PP_3259 at 3693.199 kb on + strand, within PP_3259 at 3693.200 kb on - strand, within PP_3259 at 3693.399 kb on + strand, within PP_3259 at 3693.400 kb on - strand, within PP_3259 at 3693.400 kb on - strand, within PP_3259 at 3693.554 kb on + strand at 3693.555 kb on - strand at 3693.578 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,690,902 - PP_3256 0.51 +1.2 3,691,050 - PP_3256 0.74 +0.9 3,691,100 + PP_3256 0.81 +0.3 3,691,196 - -0.5 3,691,269 - -0.8 3,691,269 - -0.3 3,691,334 - PP_3257 0.19 -0.8 3,691,413 - PP_3257 0.32 -0.9 3,691,469 + PP_3257 0.42 +0.4 3,691,469 + PP_3257 0.42 -0.1 3,691,469 + PP_3257 0.42 -1.2 3,691,470 - PP_3257 0.42 +0.3 3,691,470 - PP_3257 0.42 +1.2 3,691,470 - PP_3257 0.42 +0.2 3,691,470 - PP_3257 0.42 +1.1 3,691,470 - PP_3257 0.42 -0.2 3,691,470 - PP_3257 0.42 +0.3 3,691,472 + PP_3257 0.42 -1.8 3,691,473 - PP_3257 0.42 +0.9 3,691,473 - PP_3257 0.42 +0.3 3,691,691 + PP_3257 0.79 -1.2 3,691,692 - PP_3257 0.79 -2.4 3,691,692 - PP_3257 0.79 +0.5 3,691,742 + PP_3257 0.87 -0.6 3,691,784 + -0.2 3,691,786 + +1.0 3,691,786 + +0.7 3,691,787 - -0.1 3,691,787 - +0.3 3,691,787 - -2.3 3,691,787 - +0.2 3,691,800 - -0.5 3,692,046 + PP_3258 0.29 -0.8 3,692,461 - PP_3258 0.83 -0.7 3,692,461 - PP_3258 0.83 -1.1 3,692,585 + +0.0 3,692,585 + +1.1 3,692,585 + +0.5 3,692,586 - -0.2 3,692,586 - +0.9 3,692,586 - +2.1 3,692,586 - +1.4 3,692,586 - +0.3 3,692,750 + PP_3259 0.16 -0.7 3,692,750 + PP_3259 0.16 +0.0 3,692,750 + PP_3259 0.16 +0.1 3,692,751 - PP_3259 0.16 -0.2 3,692,774 + PP_3259 0.18 +0.3 3,693,031 - PP_3259 0.43 +0.4 3,693,065 - PP_3259 0.46 -0.0 3,693,199 + PP_3259 0.59 -3.3 3,693,200 - PP_3259 0.59 +0.0 3,693,399 + PP_3259 0.78 -0.1 3,693,400 - PP_3259 0.78 +1.6 3,693,400 - PP_3259 0.78 +0.0 3,693,554 + -0.7 3,693,555 - +0.4 3,693,578 + -1.3
Or see this region's nucleotide sequence