Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3099 and PP_3100 are separated by 14 nucleotides PP_3100 and PP_5562 are separated by 28 nucleotides
PP_3099: PP_3099 - uricase/urate oxidase, at 3,494,711 to 3,496,213
_3099
PP_3100: PP_3100 - conserved protein of unknown function, at 3,496,228 to 3,496,803
_3100
PP_5562: PP_5562 - conserved protein of unknown function, at 3,496,832 to 3,497,596
_5562
Position (kb)
3496
3497 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3495.375 kb on + strand, within PP_3099 at 3495.448 kb on - strand, within PP_3099 at 3495.531 kb on + strand, within PP_3099 at 3495.531 kb on + strand, within PP_3099 at 3495.675 kb on + strand, within PP_3099 at 3495.675 kb on + strand, within PP_3099 at 3495.675 kb on + strand, within PP_3099 at 3495.675 kb on + strand at 3495.676 kb on - strand, within PP_3099 at 3495.676 kb on - strand, within PP_3099 at 3495.676 kb on - strand, within PP_3099 at 3495.676 kb on - strand at 3495.676 kb on - strand, within PP_3099 at 3495.750 kb on + strand, within PP_3099 at 3495.762 kb on + strand, within PP_3099 at 3495.819 kb on - strand, within PP_3099 at 3496.578 kb on - strand, within PP_3100 at 3496.701 kb on - strand, within PP_3100 at 3496.707 kb on + strand, within PP_3100 at 3496.707 kb on + strand, within PP_3100 at 3496.708 kb on - strand, within PP_3100 at 3496.833 kb on + strand at 3497.101 kb on - strand, within PP_5562 at 3497.101 kb on - strand, within PP_5562 at 3497.101 kb on - strand, within PP_5562 at 3497.116 kb on - strand, within PP_5562 at 3497.133 kb on + strand, within PP_5562 at 3497.319 kb on - strand, within PP_5562 at 3497.319 kb on - strand, within PP_5562 at 3497.370 kb on + strand, within PP_5562 at 3497.370 kb on + strand, within PP_5562 at 3497.371 kb on - strand, within PP_5562 at 3497.440 kb on - strand, within PP_5562 at 3497.490 kb on + strand, within PP_5562 at 3497.679 kb on + strand at 3497.679 kb on + strand at 3497.679 kb on + strand at 3497.680 kb on - strand at 3497.685 kb on + strand at 3497.685 kb on + strand at 3497.685 kb on + strand at 3497.685 kb on + strand at 3497.686 kb on - strand at 3497.686 kb on - strand at 3497.686 kb on - strand at 3497.686 kb on - strand at 3497.686 kb on - strand at 3497.686 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,495,375 + PP_3099 0.44 -0.9 3,495,448 - PP_3099 0.49 -3.2 3,495,531 + PP_3099 0.55 -0.6 3,495,531 + PP_3099 0.55 +0.4 3,495,675 + PP_3099 0.64 +0.5 3,495,675 + PP_3099 0.64 +0.1 3,495,675 + PP_3099 0.64 +2.1 3,495,675 + +1.7 3,495,676 - PP_3099 0.64 +0.2 3,495,676 - PP_3099 0.64 -0.4 3,495,676 - PP_3099 0.64 -0.9 3,495,676 - +0.7 3,495,676 - PP_3099 0.64 -0.4 3,495,750 + PP_3099 0.69 +0.7 3,495,762 + PP_3099 0.70 -0.0 3,495,819 - PP_3099 0.74 -2.8 3,496,578 - PP_3100 0.61 -0.9 3,496,701 - PP_3100 0.82 -0.7 3,496,707 + PP_3100 0.83 -0.9 3,496,707 + PP_3100 0.83 -0.9 3,496,708 - PP_3100 0.83 -0.1 3,496,833 + +0.3 3,497,101 - PP_5562 0.35 -0.3 3,497,101 - PP_5562 0.35 +1.0 3,497,101 - PP_5562 0.35 +0.6 3,497,116 - PP_5562 0.37 +1.3 3,497,133 + PP_5562 0.39 -1.0 3,497,319 - PP_5562 0.64 -1.2 3,497,319 - PP_5562 0.64 +0.2 3,497,370 + PP_5562 0.70 +0.0 3,497,370 + PP_5562 0.70 +0.2 3,497,371 - PP_5562 0.70 -0.4 3,497,440 - PP_5562 0.79 +0.9 3,497,490 + PP_5562 0.86 -2.1 3,497,679 + -1.1 3,497,679 + -0.0 3,497,679 + -1.0 3,497,680 - -1.5 3,497,685 + -2.4 3,497,685 + -1.4 3,497,685 + -0.9 3,497,685 + +1.8 3,497,686 - +0.0 3,497,686 - -0.7 3,497,686 - -0.2 3,497,686 - +0.6 3,497,686 - -0.7 3,497,686 - -2.5
Or see this region's nucleotide sequence