Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2621 and PP_2622 are separated by 3 nucleotides PP_2622 and PP_2623 overlap by 4 nucleotides PP_2623 and PP_2624 are separated by 17 nucleotides PP_2624 and PP_2625 are separated by 281 nucleotides
PP_2621: PP_2621 - conserved protein of unknown function, at 2,996,807 to 2,998,573
_2621
PP_2622: PP_2622 - conserved protein of unknown function, at 2,998,577 to 2,998,987
_2622
PP_2623: PP_2623 - conserved protein of unknown function, at 2,998,984 to 3,000,474
_2623
PP_2624: PP_2624 - conserved protein of unknown function, at 3,000,492 to 3,000,995
_2624
PP_2625: PP_2625 - conserved exported protein of unknown function, at 3,001,277 to 3,001,972
_2625
Position (kb)
2998
2999
3000
3001 Strain fitness (log2 ratio)
-2
-1
0
1 at 2998.009 kb on - strand, within PP_2621 at 2998.009 kb on - strand, within PP_2621 at 2998.026 kb on + strand, within PP_2621 at 2998.063 kb on - strand, within PP_2621 at 2998.342 kb on - strand, within PP_2621 at 2998.410 kb on - strand at 2998.537 kb on - strand at 2998.655 kb on + strand, within PP_2622 at 2998.656 kb on - strand, within PP_2622 at 2998.725 kb on - strand, within PP_2622 at 2998.730 kb on + strand, within PP_2622 at 2999.036 kb on + strand at 2999.037 kb on - strand at 2999.037 kb on - strand at 2999.174 kb on + strand, within PP_2623 at 2999.175 kb on - strand, within PP_2623 at 2999.292 kb on - strand, within PP_2623 at 2999.292 kb on - strand, within PP_2623 at 2999.315 kb on + strand, within PP_2623 at 2999.413 kb on - strand, within PP_2623 at 2999.471 kb on - strand at 2999.648 kb on + strand, within PP_2623 at 2999.649 kb on - strand, within PP_2623 at 2999.681 kb on + strand, within PP_2623 at 2999.681 kb on + strand, within PP_2623 at 2999.842 kb on - strand, within PP_2623 at 2999.934 kb on - strand, within PP_2623 at 2999.939 kb on - strand, within PP_2623 at 2999.976 kb on - strand, within PP_2623 at 3000.044 kb on + strand, within PP_2623 at 3000.044 kb on + strand, within PP_2623 at 3000.334 kb on + strand at 3000.373 kb on + strand at 3000.373 kb on + strand at 3000.373 kb on + strand at 3000.374 kb on - strand at 3000.415 kb on - strand at 3000.563 kb on + strand, within PP_2624 at 3000.564 kb on - strand, within PP_2624 at 3000.564 kb on - strand, within PP_2624 at 3000.815 kb on + strand, within PP_2624 at 3000.815 kb on + strand, within PP_2624 at 3000.815 kb on + strand, within PP_2624 at 3000.816 kb on - strand, within PP_2624 at 3000.816 kb on - strand at 3001.051 kb on - strand at 3001.083 kb on - strand at 3001.200 kb on + strand at 3001.286 kb on - strand at 3001.338 kb on + strand at 3001.338 kb on + strand at 3001.338 kb on + strand at 3001.339 kb on - strand at 3001.340 kb on + strand at 3001.470 kb on - strand, within PP_2625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,998,009 - PP_2621 0.68 -0.0 2,998,009 - PP_2621 0.68 -0.8 2,998,026 + PP_2621 0.69 +0.2 2,998,063 - PP_2621 0.71 -0.5 2,998,342 - PP_2621 0.87 -2.4 2,998,410 - +0.5 2,998,537 - +0.9 2,998,655 + PP_2622 0.19 +0.2 2,998,656 - PP_2622 0.19 +0.4 2,998,725 - PP_2622 0.36 -0.4 2,998,730 + PP_2622 0.37 -0.6 2,999,036 + +0.4 2,999,037 - -0.1 2,999,037 - +0.1 2,999,174 + PP_2623 0.13 -0.7 2,999,175 - PP_2623 0.13 -1.5 2,999,292 - PP_2623 0.21 +0.5 2,999,292 - PP_2623 0.21 -0.4 2,999,315 + PP_2623 0.22 +0.4 2,999,413 - PP_2623 0.29 -0.6 2,999,471 - +1.5 2,999,648 + PP_2623 0.45 -1.5 2,999,649 - PP_2623 0.45 -0.7 2,999,681 + PP_2623 0.47 +0.1 2,999,681 + PP_2623 0.47 -0.6 2,999,842 - PP_2623 0.58 -0.7 2,999,934 - PP_2623 0.64 -0.3 2,999,939 - PP_2623 0.64 -1.3 2,999,976 - PP_2623 0.67 +0.5 3,000,044 + PP_2623 0.71 +0.7 3,000,044 + PP_2623 0.71 -2.5 3,000,334 + -1.5 3,000,373 + -1.0 3,000,373 + -0.3 3,000,373 + -0.2 3,000,374 - +1.0 3,000,415 - -0.9 3,000,563 + PP_2624 0.14 +1.1 3,000,564 - PP_2624 0.14 -2.4 3,000,564 - PP_2624 0.14 +1.3 3,000,815 + PP_2624 0.64 +1.0 3,000,815 + PP_2624 0.64 -0.9 3,000,815 + PP_2624 0.64 -0.3 3,000,816 - PP_2624 0.64 -1.1 3,000,816 - -0.1 3,001,051 - -0.1 3,001,083 - -0.5 3,001,200 + +0.1 3,001,286 - -0.1 3,001,338 + -0.6 3,001,338 + +0.1 3,001,338 + +1.0 3,001,339 - +0.5 3,001,340 + +0.2 3,001,470 - PP_2625 0.28 -1.6
Or see this region's nucleotide sequence