Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1217 and PP_1218 are separated by 125 nucleotides PP_1218 and PP_1219 are separated by 2 nucleotides PP_1219 and PP_1220 are separated by 14 nucleotides
PP_1217: PP_1217 - Holliday junction ATP-dependent DNA helicase RuvB, at 1,394,264 to 1,395,310
_1217
PP_1218: PP_1218 - putative acyl-CoA thioesterase, at 1,395,436 to 1,395,888
_1218
PP_1219: PP_1219 - subunit of a proton-driven complex used for maintenance of outer membrane stability, at 1,395,891 to 1,396,586
_1219
PP_1220: PP_1220 - subunit of a proton-driven complex used for maintenance of outer membrane stability, at 1,396,601 to 1,397,053
_1220
Position (kb)
1395
1396 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1394.705 kb on - strand, within PP_1217 at 1394.744 kb on - strand, within PP_1217 at 1394.747 kb on - strand, within PP_1217 at 1394.814 kb on - strand, within PP_1217 at 1394.891 kb on + strand, within PP_1217 at 1394.961 kb on - strand, within PP_1217 at 1395.148 kb on + strand, within PP_1217 at 1395.148 kb on + strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.173 kb on + strand, within PP_1217 at 1395.191 kb on + strand, within PP_1217 at 1395.191 kb on + strand, within PP_1217 at 1395.191 kb on + strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.236 kb on + strand at 1395.236 kb on + strand at 1395.237 kb on - strand at 1395.237 kb on - strand at 1395.237 kb on - strand at 1395.256 kb on + strand at 1395.256 kb on + strand at 1395.257 kb on - strand at 1395.257 kb on - strand at 1395.257 kb on - strand at 1395.377 kb on + strand at 1395.377 kb on + strand at 1395.423 kb on + strand at 1395.423 kb on + strand at 1395.430 kb on + strand at 1395.430 kb on + strand at 1395.495 kb on + strand, within PP_1218 at 1395.517 kb on + strand, within PP_1218 at 1395.517 kb on + strand, within PP_1218 at 1395.520 kb on + strand, within PP_1218 at 1395.520 kb on + strand, within PP_1218 at 1395.725 kb on + strand, within PP_1218 at 1395.821 kb on + strand, within PP_1218 at 1396.584 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,394,705 - PP_1217 0.42 -2.1 1,394,744 - PP_1217 0.46 -1.9 1,394,747 - PP_1217 0.46 +0.4 1,394,814 - PP_1217 0.53 -0.1 1,394,891 + PP_1217 0.60 -1.5 1,394,961 - PP_1217 0.67 -1.9 1,395,148 + PP_1217 0.84 -0.2 1,395,148 + PP_1217 0.84 +0.1 1,395,149 - PP_1217 0.85 -1.9 1,395,149 - PP_1217 0.85 -2.7 1,395,149 - PP_1217 0.85 -1.7 1,395,149 - PP_1217 0.85 -3.3 1,395,149 - PP_1217 0.85 -2.5 1,395,173 + PP_1217 0.87 -2.0 1,395,191 + PP_1217 0.89 -2.6 1,395,191 + PP_1217 0.89 -0.1 1,395,191 + PP_1217 0.89 -1.2 1,395,192 - PP_1217 0.89 -2.9 1,395,192 - PP_1217 0.89 -2.0 1,395,192 - PP_1217 0.89 -1.8 1,395,192 - PP_1217 0.89 -1.3 1,395,236 + -2.7 1,395,236 + +0.5 1,395,237 - -0.3 1,395,237 - -2.5 1,395,237 - -1.1 1,395,256 + +0.3 1,395,256 + -0.2 1,395,257 - -3.5 1,395,257 - -2.9 1,395,257 - -0.6 1,395,377 + +0.5 1,395,377 + +0.6 1,395,423 + +0.3 1,395,423 + +0.3 1,395,430 + -1.3 1,395,430 + +0.8 1,395,495 + PP_1218 0.13 -0.8 1,395,517 + PP_1218 0.18 -2.4 1,395,517 + PP_1218 0.18 +1.5 1,395,520 + PP_1218 0.19 -1.8 1,395,520 + PP_1218 0.19 -0.8 1,395,725 + PP_1218 0.64 -2.3 1,395,821 + PP_1218 0.85 -0.3 1,396,584 + +1.1
Or see this region's nucleotide sequence