Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1064 and PP_1065 are separated by 17 nucleotides PP_1065 and PP_1066 are separated by 23 nucleotides PP_1066 and PP_1067 overlap by 4 nucleotides
PP_1064: PP_1064 - Hydrolase, alpha/beta fold family, at 1,218,464 to 1,219,600
_1064
PP_1065: PP_1065 - membrane protein required for efficient alginate biosynthesis, at 1,219,618 to 1,219,953
_1065
PP_1066: PP_1066 - C4-dicarboxylate transport transcriptional regulatory protein, at 1,219,977 to 1,221,305
_1066
PP_1067: PP_1067 - Sensor histidine kinase, at 1,221,302 to 1,223,209
_1067
Position (kb)
1219
1220
1221
1222 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1219.029 kb on + strand, within PP_1064 at 1219.030 kb on - strand, within PP_1064 at 1219.252 kb on - strand, within PP_1064 at 1219.252 kb on - strand, within PP_1064 at 1219.296 kb on - strand, within PP_1064 at 1219.404 kb on + strand, within PP_1064 at 1219.404 kb on + strand, within PP_1064 at 1219.425 kb on + strand, within PP_1064 at 1219.554 kb on - strand at 1219.554 kb on - strand at 1219.720 kb on + strand, within PP_1065 at 1219.720 kb on + strand, within PP_1065 at 1219.720 kb on + strand, within PP_1065 at 1219.721 kb on - strand, within PP_1065 at 1219.721 kb on - strand, within PP_1065 at 1219.721 kb on - strand, within PP_1065 at 1219.721 kb on - strand, within PP_1065 at 1219.721 kb on - strand, within PP_1065 at 1219.721 kb on - strand, within PP_1065 at 1219.724 kb on + strand, within PP_1065 at 1219.724 kb on + strand, within PP_1065 at 1219.724 kb on + strand, within PP_1065 at 1219.724 kb on + strand, within PP_1065 at 1219.724 kb on + strand, within PP_1065 at 1219.725 kb on - strand, within PP_1065 at 1219.725 kb on - strand, within PP_1065 at 1219.725 kb on - strand, within PP_1065 at 1219.725 kb on - strand, within PP_1065 at 1219.823 kb on + strand, within PP_1065 at 1219.823 kb on + strand, within PP_1065 at 1219.824 kb on - strand, within PP_1065 at 1219.824 kb on - strand, within PP_1065 at 1219.824 kb on - strand, within PP_1065 at 1219.824 kb on - strand, within PP_1065 at 1219.874 kb on + strand, within PP_1065 at 1219.875 kb on - strand, within PP_1065 at 1219.875 kb on - strand, within PP_1065 at 1219.877 kb on + strand, within PP_1065 at 1219.877 kb on + strand, within PP_1065 at 1219.877 kb on + strand, within PP_1065 at 1219.878 kb on - strand, within PP_1065 at 1219.878 kb on - strand, within PP_1065 at 1219.878 kb on - strand, within PP_1065 at 1219.991 kb on - strand at 1220.185 kb on + strand, within PP_1066 at 1220.392 kb on + strand, within PP_1066 at 1220.392 kb on + strand, within PP_1066 at 1220.392 kb on + strand, within PP_1066 at 1220.955 kb on + strand, within PP_1066 at 1220.956 kb on - strand, within PP_1066 at 1221.014 kb on + strand, within PP_1066 at 1221.201 kb on + strand at 1221.218 kb on + strand at 1221.287 kb on + strand at 1221.546 kb on + strand, within PP_1067 at 1221.546 kb on + strand, within PP_1067 at 1221.546 kb on + strand, within PP_1067 at 1221.547 kb on - strand, within PP_1067 at 1221.579 kb on - strand, within PP_1067 at 1221.749 kb on + strand, within PP_1067 at 1221.907 kb on + strand, within PP_1067 at 1221.907 kb on + strand, within PP_1067 at 1221.907 kb on + strand, within PP_1067 at 1221.908 kb on - strand, within PP_1067 at 1221.908 kb on - strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,219,029 + PP_1064 0.50 +0.5 1,219,030 - PP_1064 0.50 +0.9 1,219,252 - PP_1064 0.69 -0.4 1,219,252 - PP_1064 0.69 +0.4 1,219,296 - PP_1064 0.73 +1.7 1,219,404 + PP_1064 0.83 +0.5 1,219,404 + PP_1064 0.83 +0.3 1,219,425 + PP_1064 0.85 -0.9 1,219,554 - -1.0 1,219,554 - -0.9 1,219,720 + PP_1065 0.30 -1.3 1,219,720 + PP_1065 0.30 -3.2 1,219,720 + PP_1065 0.30 +0.9 1,219,721 - PP_1065 0.31 +0.1 1,219,721 - PP_1065 0.31 -0.6 1,219,721 - PP_1065 0.31 +0.7 1,219,721 - PP_1065 0.31 +0.9 1,219,721 - PP_1065 0.31 +0.2 1,219,721 - PP_1065 0.31 +0.1 1,219,724 + PP_1065 0.32 -0.4 1,219,724 + PP_1065 0.32 -2.1 1,219,724 + PP_1065 0.32 -1.6 1,219,724 + PP_1065 0.32 +0.3 1,219,724 + PP_1065 0.32 -1.5 1,219,725 - PP_1065 0.32 -0.5 1,219,725 - PP_1065 0.32 +1.1 1,219,725 - PP_1065 0.32 -0.4 1,219,725 - PP_1065 0.32 +0.2 1,219,823 + PP_1065 0.61 -1.2 1,219,823 + PP_1065 0.61 -0.3 1,219,824 - PP_1065 0.61 -0.2 1,219,824 - PP_1065 0.61 -1.3 1,219,824 - PP_1065 0.61 +0.5 1,219,824 - PP_1065 0.61 -0.7 1,219,874 + PP_1065 0.76 -0.3 1,219,875 - PP_1065 0.76 +0.6 1,219,875 - PP_1065 0.76 -0.1 1,219,877 + PP_1065 0.77 +0.2 1,219,877 + PP_1065 0.77 +0.0 1,219,877 + PP_1065 0.77 -0.9 1,219,878 - PP_1065 0.77 -0.8 1,219,878 - PP_1065 0.77 +0.7 1,219,878 - PP_1065 0.77 -0.5 1,219,991 - -0.8 1,220,185 + PP_1066 0.16 +1.1 1,220,392 + PP_1066 0.31 -0.3 1,220,392 + PP_1066 0.31 -0.6 1,220,392 + PP_1066 0.31 -2.3 1,220,955 + PP_1066 0.74 +1.3 1,220,956 - PP_1066 0.74 +0.5 1,221,014 + PP_1066 0.78 +1.3 1,221,201 + +1.5 1,221,218 + -0.7 1,221,287 + -0.2 1,221,546 + PP_1067 0.13 +0.9 1,221,546 + PP_1067 0.13 +0.1 1,221,546 + PP_1067 0.13 -1.1 1,221,547 - PP_1067 0.13 +0.5 1,221,579 - PP_1067 0.15 -1.2 1,221,749 + PP_1067 0.23 +0.6 1,221,907 + PP_1067 0.32 -0.8 1,221,907 + PP_1067 0.32 -2.3 1,221,907 + PP_1067 0.32 +0.6 1,221,908 - PP_1067 0.32 +0.2 1,221,908 - PP_1067 0.32 -1.9 1,221,994 + PP_1067 0.36 +0.3 1,221,994 + PP_1067 0.36 +0.6 1,221,994 + PP_1067 0.36 -0.3 1,221,994 + PP_1067 0.36 -0.4
Or see this region's nucleotide sequence