Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0832 and PP_0833 are separated by 14 nucleotides PP_0833 and PP_0834 are separated by 42 nucleotides PP_0834 and PP_0835 are separated by 63 nucleotides
PP_0832: PP_0832 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 969,088 to 970,137
_0832
PP_0833: PP_0833 - queuine tRNA-ribosyltransferase, at 970,152 to 971,267
_0833
PP_0834: PP_0834 - Sec translocation complex subunit YajC, at 971,310 to 971,645
_0834
PP_0835: PP_0835 - Sec Translocation Complex SecD subunit, at 971,709 to 973,571
_0835
Position (kb) 970
971
972 Strain fitness (log2 ratio) -2
-1
0
1
2 at 969.232 kb on + strand, within PP_0832 at 969.232 kb on + strand, within PP_0832 at 969.233 kb on - strand, within PP_0832 at 969.395 kb on + strand, within PP_0832 at 969.396 kb on - strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.531 kb on + strand, within PP_0832 at 969.532 kb on - strand, within PP_0832 at 969.569 kb on - strand, within PP_0832 at 969.753 kb on + strand, within PP_0832 at 970.037 kb on - strand at 970.076 kb on - strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.435 kb on + strand, within PP_0833 at 970.567 kb on + strand, within PP_0833 at 970.716 kb on + strand, within PP_0833 at 970.955 kb on + strand, within PP_0833 at 970.985 kb on + strand, within PP_0833 at 971.060 kb on + strand, within PP_0833 at 971.085 kb on + strand, within PP_0833 at 971.135 kb on + strand, within PP_0833 at 971.201 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 969,232 + PP_0832 0.14 -2.4 969,232 + PP_0832 0.14 -1.2 969,233 - PP_0832 0.14 -2.3 969,395 + PP_0832 0.29 +1.0 969,396 - PP_0832 0.29 -1.1 969,529 + PP_0832 0.42 +0.1 969,529 + PP_0832 0.42 +0.8 969,529 + PP_0832 0.42 -0.7 969,531 + PP_0832 0.42 -0.3 969,532 - PP_0832 0.42 +0.9 969,569 - PP_0832 0.46 -1.5 969,753 + PP_0832 0.63 +0.1 970,037 - +2.0 970,076 - -0.1 970,254 + -2.2 970,254 + -2.1 970,254 + -0.6 970,255 - -1.9 970,255 - -1.6 970,255 - -1.2 970,435 + PP_0833 0.25 +1.7 970,567 + PP_0833 0.37 -1.1 970,716 + PP_0833 0.51 -2.4 970,955 + PP_0833 0.72 +1.6 970,985 + PP_0833 0.75 -1.0 971,060 + PP_0833 0.81 -1.2 971,085 + PP_0833 0.84 -2.0 971,135 + PP_0833 0.88 +0.1 971,201 + -1.6 971,298 + +0.7 971,298 + -0.1 971,298 + -0.2 971,418 + PP_0834 0.32 -1.0 971,418 + PP_0834 0.32 -0.1 971,418 + PP_0834 0.32 +0.4 971,639 + +0.3
Or see this region's nucleotide sequence