Strain Fitness in Pseudomonas putida KT2440 around PP_0240

Experiment: R2A with Fusaric 120 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0238 and PP_0239 are separated by 10 nucleotidesPP_0239 and PP_0240 overlap by 4 nucleotidesPP_0240 and PP_0241 are separated by 27 nucleotidesPP_0241 and PP_0242 are separated by 141 nucleotides PP_0238: PP_0238 - alkanesulfonate monooxygenase, at 292,644 to 293,792 _0238 PP_0239: PP_0239 - aliphatic sulfonate ABC transporter - permease subunit / transport of isethionate, at 293,803 to 294,600 _0239 PP_0240: PP_0240 - Aliphatic sulfonates import ATP-binding protein SsuB, at 294,597 to 295,409 _0240 PP_0241: PP_0241 - Transporter, at 295,437 to 295,652 _0241 PP_0242: PP_0242 - Transcriptional regulator, TetR family, at 295,794 to 296,426 _0242 Position (kb) 294 295 296Strain fitness (log2 ratio) -2 -1 0 1at 293.599 kb on - strand, within PP_0238at 293.618 kb on + strandat 293.618 kb on + strand, within PP_0238at 293.618 kb on + strand, within PP_0238at 293.619 kb on - strand, within PP_0238at 293.619 kb on - strand, within PP_0238at 293.659 kb on - strand, within PP_0238at 293.727 kb on + strandat 293.728 kb on - strandat 293.731 kb on + strandat 293.731 kb on + strandat 293.732 kb on - strandat 293.732 kb on - strandat 293.732 kb on - strandat 293.732 kb on - strandat 293.877 kb on - strandat 293.916 kb on + strand, within PP_0239at 293.931 kb on + strand, within PP_0239at 293.931 kb on + strand, within PP_0239at 294.108 kb on - strand, within PP_0239at 294.223 kb on + strand, within PP_0239at 294.231 kb on + strand, within PP_0239at 294.232 kb on - strand, within PP_0239at 294.236 kb on - strand, within PP_0239at 294.250 kb on - strand, within PP_0239at 294.287 kb on - strand, within PP_0239at 294.476 kb on + strand, within PP_0239at 294.496 kb on + strand, within PP_0239at 294.496 kb on + strand, within PP_0239at 294.497 kb on - strand, within PP_0239at 294.572 kb on - strandat 294.572 kb on - strandat 295.168 kb on + strand, within PP_0240at 295.168 kb on + strand, within PP_0240at 295.169 kb on - strand, within PP_0240at 295.169 kb on - strand, within PP_0240at 295.372 kb on + strandat 295.373 kb on - strandat 295.442 kb on + strandat 295.443 kb on - strandat 295.443 kb on - strandat 295.443 kb on - strandat 295.478 kb on + strand, within PP_0241at 295.479 kb on - strand, within PP_0241at 295.559 kb on + strand, within PP_0241at 295.560 kb on - strand, within PP_0241at 295.637 kb on + strandat 295.637 kb on + strandat 295.828 kb on + strandat 295.829 kb on - strandat 295.829 kb on - strandat 295.840 kb on + strandat 295.841 kb on - strandat 295.841 kb on - strandat 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 296.053 kb on - strand, within PP_0242at 296.110 kb on + strand, within PP_0242at 296.110 kb on + strand, within PP_0242at 296.111 kb on - strand, within PP_0242at 296.111 kb on - strand, within PP_0242at 296.114 kb on + strand, within PP_0242at 296.115 kb on - strand, within PP_0242at 296.115 kb on - strand, within PP_0242at 296.225 kb on + strand, within PP_0242at 296.225 kb on + strand, within PP_0242at 296.226 kb on - strand, within PP_0242at 296.226 kb on - strand, within PP_0242at 296.308 kb on + strand, within PP_0242at 296.308 kb on + strand, within PP_0242at 296.312 kb on + strand, within PP_0242

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL
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293,599 - PP_0238 0.83 +0.3
293,618 + +1.4
293,618 + PP_0238 0.85 +1.2
293,618 + PP_0238 0.85 +0.8
293,619 - PP_0238 0.85 -0.2
293,619 - PP_0238 0.85 +0.1
293,659 - PP_0238 0.88 -0.6
293,727 + -0.1
293,728 - +0.3
293,731 + -0.3
293,731 + +0.7
293,732 - +0.8
293,732 - -0.4
293,732 - -0.3
293,732 - -0.2
293,877 - +0.1
293,916 + PP_0239 0.14 -0.6
293,931 + PP_0239 0.16 +1.0
293,931 + PP_0239 0.16 +0.9
294,108 - PP_0239 0.38 +1.4
294,223 + PP_0239 0.53 -0.1
294,231 + PP_0239 0.54 +0.3
294,232 - PP_0239 0.54 -0.6
294,236 - PP_0239 0.54 -1.5
294,250 - PP_0239 0.56 -1.0
294,287 - PP_0239 0.61 -0.2
294,476 + PP_0239 0.84 -0.8
294,496 + PP_0239 0.87 +0.5
294,496 + PP_0239 0.87 +1.3
294,497 - PP_0239 0.87 -0.2
294,572 - +0.2
294,572 - -0.8
295,168 + PP_0240 0.70 +0.5
295,168 + PP_0240 0.70 -1.6
295,169 - PP_0240 0.70 -1.4
295,169 - PP_0240 0.70 -0.4
295,372 + -0.5
295,373 - +0.1
295,442 + -0.4
295,443 - -0.2
295,443 - +1.0
295,443 - +1.1
295,478 + PP_0241 0.19 -1.0
295,479 - PP_0241 0.19 +0.8
295,559 + PP_0241 0.56 -0.3
295,560 - PP_0241 0.57 +0.7
295,637 + -0.7
295,637 + -0.1
295,828 + -0.3
295,829 - +1.1
295,829 - +0.4
295,840 + +0.8
295,841 - +0.1
295,841 - +1.1
295,915 + PP_0242 0.19 -0.3
295,915 + PP_0242 0.19 +0.1
295,915 + PP_0242 0.19 -1.1
295,915 + PP_0242 0.19 +0.2
295,915 + PP_0242 0.19 +0.0
295,916 - PP_0242 0.19 +0.6
295,916 - PP_0242 0.19 +1.1
295,916 - PP_0242 0.19 +0.3
295,916 - PP_0242 0.19 +0.2
295,916 - PP_0242 0.19 -0.5
295,916 - PP_0242 0.19 +0.3
296,053 - PP_0242 0.41 -0.7
296,110 + PP_0242 0.50 +0.6
296,110 + PP_0242 0.50 -2.0
296,111 - PP_0242 0.50 +0.3
296,111 - PP_0242 0.50 -1.4
296,114 + PP_0242 0.51 +0.8
296,115 - PP_0242 0.51 -1.2
296,115 - PP_0242 0.51 -1.3
296,225 + PP_0242 0.68 -0.7
296,225 + PP_0242 0.68 -1.8
296,226 - PP_0242 0.68 -0.7
296,226 - PP_0242 0.68 +0.9
296,308 + PP_0242 0.81 -0.1
296,308 + PP_0242 0.81 -1.8
296,312 + PP_0242 0.82 -0.4

Or see this region's nucleotide sequence