Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5233 and PP_5234 are separated by 46 nucleotides PP_5234 and PP_5723 are separated by 134 nucleotides PP_5723 and PP_5235 are separated by 24 nucleotides PP_5235 and PP_5724 are separated by 0 nucleotides PP_5724 and PP_5236 overlap by 47 nucleotides
PP_5233: PP_5233 - ammonium transporter AmtB, at 5,966,361 to 5,967,692
_5233
PP_5234: PP_5234 - activator of NRII(GlnL/NtrB) phosphatase, at 5,967,739 to 5,968,077
_5234
PP_5723: PP_5723 - protein of unknown function, at 5,968,212 to 5,968,571
_5723
PP_5235: PP_5235 - conserved protein of unknown function, at 5,968,596 to 5,968,862
_5235
PP_5724: PP_5724 - conserved protein of unknown function, at 5,968,863 to 5,969,216
_5724
PP_5236: PP_5236 - conserved protein of unknown function, at 5,969,170 to 5,971,293
_5236
Position (kb)
5968
5969 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5967.433 kb on + strand, within PP_5233 at 5967.523 kb on + strand, within PP_5233 at 5967.523 kb on + strand, within PP_5233 at 5967.524 kb on - strand, within PP_5233 at 5967.524 kb on - strand, within PP_5233 at 5967.545 kb on + strand, within PP_5233 at 5967.546 kb on - strand, within PP_5233 at 5967.602 kb on + strand at 5967.603 kb on - strand at 5967.645 kb on + strand at 5967.645 kb on + strand at 5967.657 kb on + strand at 5967.658 kb on - strand at 5967.666 kb on + strand at 5967.667 kb on - strand at 5967.667 kb on - strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.741 kb on - strand at 5967.741 kb on - strand at 5967.741 kb on - strand at 5968.135 kb on - strand at 5968.228 kb on - strand at 5968.283 kb on + strand, within PP_5723 at 5968.283 kb on + strand, within PP_5723 at 5968.284 kb on - strand at 5968.284 kb on - strand, within PP_5723 at 5968.470 kb on + strand, within PP_5723 at 5968.471 kb on - strand, within PP_5723 at 5968.471 kb on - strand, within PP_5723 at 5968.557 kb on + strand at 5968.557 kb on + strand at 5968.772 kb on - strand, within PP_5235 at 5968.881 kb on + strand at 5968.889 kb on - strand at 5968.889 kb on - strand at 5968.902 kb on + strand, within PP_5724 at 5968.902 kb on + strand, within PP_5724 at 5968.903 kb on - strand, within PP_5724 at 5968.973 kb on + strand, within PP_5724 at 5968.974 kb on - strand, within PP_5724 at 5968.974 kb on - strand, within PP_5724 at 5968.974 kb on - strand, within PP_5724 at 5969.154 kb on + strand, within PP_5724 at 5969.154 kb on + strand, within PP_5724 at 5969.155 kb on - strand, within PP_5724 at 5969.202 kb on + strand at 5969.202 kb on + strand at 5969.203 kb on - strand at 5969.203 kb on - strand at 5969.278 kb on + strand at 5969.278 kb on + strand at 5969.278 kb on + strand at 5969.279 kb on - strand at 5969.279 kb on - strand at 5969.279 kb on - strand at 5969.279 kb on - strand at 5969.328 kb on + strand at 5969.549 kb on + strand, within PP_5236 at 5969.550 kb on - strand, within PP_5236
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 5,967,433 + PP_5233 0.80 +0.5 5,967,523 + PP_5233 0.87 +0.0 5,967,523 + PP_5233 0.87 +0.6 5,967,524 - PP_5233 0.87 +0.0 5,967,524 - PP_5233 0.87 -0.7 5,967,545 + PP_5233 0.89 +1.3 5,967,546 - PP_5233 0.89 +0.6 5,967,602 + -0.2 5,967,603 - -0.1 5,967,645 + +0.4 5,967,645 + +0.1 5,967,657 + -0.4 5,967,658 - +0.8 5,967,666 + -0.0 5,967,667 - +0.5 5,967,667 - +0.6 5,967,740 + -1.2 5,967,740 + -1.0 5,967,740 + +0.4 5,967,740 + -1.3 5,967,740 + +0.1 5,967,741 - -1.2 5,967,741 - -1.6 5,967,741 - -1.0 5,968,135 - +0.2 5,968,228 - -0.9 5,968,283 + PP_5723 0.20 -0.2 5,968,283 + PP_5723 0.20 +0.6 5,968,284 - +2.5 5,968,284 - PP_5723 0.20 +0.3 5,968,470 + PP_5723 0.72 +0.7 5,968,471 - PP_5723 0.72 +1.3 5,968,471 - PP_5723 0.72 +0.3 5,968,557 + -0.6 5,968,557 + -0.2 5,968,772 - PP_5235 0.66 +1.2 5,968,881 + +0.7 5,968,889 - +0.6 5,968,889 - -0.5 5,968,902 + PP_5724 0.11 -0.2 5,968,902 + PP_5724 0.11 +0.9 5,968,903 - PP_5724 0.11 +1.3 5,968,973 + PP_5724 0.31 +1.3 5,968,974 - PP_5724 0.31 -0.3 5,968,974 - PP_5724 0.31 -1.5 5,968,974 - PP_5724 0.31 -0.1 5,969,154 + PP_5724 0.82 +0.4 5,969,154 + PP_5724 0.82 -2.4 5,969,155 - PP_5724 0.82 -0.1 5,969,202 + -0.8 5,969,202 + +1.7 5,969,203 - +0.9 5,969,203 - -0.5 5,969,278 + +0.6 5,969,278 + -0.7 5,969,278 + +0.4 5,969,279 - -0.2 5,969,279 - -0.4 5,969,279 - +0.4 5,969,279 - +0.1 5,969,328 + -0.4 5,969,549 + PP_5236 0.18 +1.4 5,969,550 - PP_5236 0.18 +0.6
Or see this region's nucleotide sequence